Reviewed, UniProtKB/Swiss-Prot P21913 (DHSB_RAT)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name(s)=Ip

Gene names

Name:	Sdhb
Synonyms:	Sdh1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	143 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Forms part of complex II containing four subunits: a flavoprotein (FP), an iron-sulfur protein (IP) and a cytochrome b composed of two integral membrane proteins By similarity.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	‹1 – ›143	›143	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit
Regions
	Domain	‹1 – 27	›27	2Fe-2S ferredoxin-type
	Domain	70 – 100	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	7	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	80	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	86	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	90	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	137	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	143	1	Iron-sulfur 3 (3Fe-4S) By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	143	1

Sequences

	Sequence		Length	Mass (Da)
	P21913-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: CB9D59670007BC3A	FASTA	143	16,788
	10 20 30 40 50 60 GGNTLACTRR IDTNLNKVSK IYPLPHMYVI KDLVPDLSNF YAQYRSIEPY LKKKDESQEG 70 80 90 100 110 120 KQQYLQSIEE REKLDGLYEC ILCACCSTSC PSYWWNGDKY LGPAVLMQAY RWMIDSRDDF 130 140 TEERLAKLQD PFSLYRCHTI MNC

References

[1]	"Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species." Gould S.J., Subramani S., Scheffler I.E. Proc. Natl. Acad. Sci. U.S.A. 86:1934-1938(1989) [PubMed: 2494655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Erratum Gould S.J., Subramani S., Scheffler I.E. Proc. Natl. Acad. Sci. U.S.A. 90:2556-2556(1993)

Cross-references

Sequence databases
PIR	B32394.
UniGene	Rn.3902
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
SMR	P21913. Positions 1-143.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000007967. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	1308598. Sdhb.
GeneLynx	Search...
Phylogenomic databases
HOVERGEN	P21913.
Gene expression databases
ArrayExpress	P21913.
GermOnline	ENSRNOG00000007967. Rattus norvegicus.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. Partial match. PS51085. 2FE2S_FER_2. Partial match. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB_RAT

Accession

Primary (citable) accession number: P21913

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	July 22, 2008
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)