Reviewed, UniProtKB/Swiss-Prot P22434 (PDE1_YEAST)

Names and origin

Protein names

Recommended name:
    3',5'-cyclic-nucleotide phosphodiesterase 1
      Short name=PDEase 1
    EC=3.1.4.17
Alternative name(s):
    Low-affinity cAMP phosphodiesterase
    3':5'-CNP

Gene names

Name:	PDE1
Ordered Locus Names:	YGL248W
ORF Names:	NRB369

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Controls the level of cAMP in yeast cells, together with the high-affinity cAMP phosphodiesterase (PDE2).
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate.
Miscellaneous	Present with 1400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cAMP phosphodiesterase class-II family.

Ontologies

Keywords
Ligand	cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cAMP-mediated signaling Inferred from mutant phenotype. Source: SGD
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 369	369	3',5'-cyclic-nucleotide phosphodiesterase 1
Amino acid modifications
	Modified residue	311	1	Phosphotyrosine
	Modified residue	313	1	Phosphoserine
	Modified residue	314	1	Phosphoserine
Experimental info
	Sequence conflict	94	1	L → F in AAA34896. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22434-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 47B752477E99BA88	FASTA	369	42,016
	10 20 30 40 50 60 MVVFEITILG ANGGPTEYGT QCFILKPART EDPELIAVDG GAGMYQLREM LVQGRNENEG 70 80 90 100 110 120 DDELVPSFYE HDREPIEFFI DSKLNIQKGL SKSLLQSLKR QGEHFESANT MKKTYEVFQG 130 140 150 160 170 180 ITDYYITHPH LDHISGLVVN SPSIYEQENS KKKTIWGLPH TIDVLQKHVF NDLIWPDLTA 190 200 210 220 230 240 ERSRKLKLKC LNPKEVQKCT IFPWDVIPFK VHHGIGVKTG APVYSTFYIF RDRKSKDCII 250 260 270 280 290 300 VCGDVEQDRR ESEESLLEEF WSYVAENIPL VHLKGILVEC SCPLSSKPEQ LYGHLSPIYL 310 320 330 340 350 360 INELSNLNTL YNSSKGLSGL NVIVTHVKST PAKRDPRLTI LEELRFLAEE RNLGDLRISI ALEGHTLFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae." Nikawa J., Sass P., Wigler M. Mol. Cell. Biol. 7:3629-3636(1987) [PubMed: 2824992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed: 8972578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-311; SER-313 AND SER-314, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17781 Genomic DNA. Translation: AAA34896.1. X94357 Genomic DNA. Translation: CAA64139.1. Z72770 Genomic DNA. Translation: CAA96968.1.
PIR	ESBYPC. S61613.
RefSeq	NP_011266.1.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	YGL248W. Saccharomyces cerevisiae. [Contig view]
GeneID	852644.
GenomeReviews	Gene locus YGL248W in contig Y13135_GR.
KEGG	sce:YGL248W.
NMPDR	fig|4932.3.peg.2369.
Organism-specific databases
CYGD	YGL248w.
SGD	S000003217. PDE1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P22434.
Gene expression databases
GermOnline	YGL248W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000396. Pdiesterase2. [Graphical view]
Pfam	PF02112. PDEase_II. 1 hit. [Graphical view]
PIRSF	PIRSF000962. Cyc_nuc_PDEase. 1 hit.
PRINTS	PR00388. PDIESTERASE2.
ProDom	PD010003. Pdiesterase2_lac. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00607. PDEASE_II. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	P22434.
ProtoNet	Search...

Entry information

Entry name

PDE1_YEAST

Accession

Primary (citable) accession number: P22434

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	October 1, 1996
Last modified:	September 2, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)