Reviewed, UniProtKB/Swiss-Prot P22943 (HSP12_YEAST)

Names and origin

Protein names

Recommended name:
    12 kDa heat shock protein
Alternative name(s):
    Glucose and lipid-regulated protein

Gene names

Name:	HSP12
Synonyms:	GLP1, HOR5
Ordered Locus Names:	YFL014W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in a switch from carbohydrate utilizing metabolism to fatty acid utilizing metabolism.
Induction	Strong, by heat shock, by entry in the stationary growth phase, and by cAMP, probably via the activity of a cAMP-dependent protein kinase. By glucose starvation and by fatty acids.
Miscellaneous	Present with 4490 molecules/cell in log phase SD medium.
Sequence similarities	To S.pombe hsp9 and C.albicans WH11.

Ontologies

Keywords
Biological process	Stress response
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Ref.6 Inferred from direct assay. Source: SGD hyperosmotic response Traceable author statement. Source: SGD response to heat Traceable author statement. Source: SGD response to oxidative stress Traceable author statement. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CPR1	P14832	1	EBI-8548,EBI-5463

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 109	109	12 kDa heat shock protein
Amino acid modifications
	Modified residue	21	1	Phosphoserine; by ATM or ATR
	Modified residue	24	1	Phosphoserine
	Modified residue	59	1	Phosphoserine
	Modified residue	73	1	Phosphoserine
	Modified residue	87	1	Phosphoserine
	Modified residue	97	1	Phosphoserine
Natural variations
	Natural variant	46	1	P → T in strain: Ar5-H12.

Sequences

	Sequence		Length	Mass (Da)
	P22943-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 7C73862689759B4A	FASTA	109	11,693
	10 20 30 40 50 60 MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA 70 80 90 100 EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and chromosomal assignment of a gene from Saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids." Stone R.L., Matarese V., Magee B.B., Magee P.T., Bernlohr D.A. Gene 96:171-176(1990) [PubMed: 2129531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 204508 / S288c.
[2]	"HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function." Praekelt U.M., Meacock P.A. Mol. Gen. Genet. 223:97-106(1990) [PubMed: 2175390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204508 / S288c.
[3]	Barrell B.G., Churcher C., Rajandream M.A. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204511 / S288c / AB972.
[4]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Transcriptional regulation of bottom-fermenting yeast specific HSP12 gene." Sone H., Tomizuka K., Suda H., Iwamatsu A., Kondo K., Inouye M., Tanaka J. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: KBY001.
[6]	"HSP12 is essential for biofilm formation by a Sardinian wine strain of Saccharomyces cerevisiae." Zara S., Farris G.A., Budroni M., Bakalinsky A.T. Yeast 19:269-276(2002) [PubMed: 11816034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Ar5-H12 / Arvisionadu wine isolate.
[7]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21 AND SER-24, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-24; SER-59; SER-73 AND SER-87, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-24; SER-59; SER-73; SER-87 AND SER-97, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60827 Genomic DNA. Translation: AAA34647.1. X55785 mRNA. Translation: CAA39306.1. Z46255 Genomic DNA. Translation: CAA86349.1. D50617 Genomic DNA. Translation: BAA09224.1. D89864 Genomic DNA. Translation: BAA14033.1. AY046957 Genomic DNA. Translation: AAL06077.1. AY558464 Genomic DNA. Translation: AAS56790.1.
PIR	HHBY12. S11179.
RefSeq	NP_116640.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6408N.
IntAct	P22943.
Proteomic databases
PeptideAtlas	P22943.
Genome annotation databases
Ensembl	YFL014W. Saccharomyces cerevisiae. [Contig view]
GeneID	850532.
GenomeReviews	Gene locus YFL014W in contig D50617_GR.
KEGG	sce:YFL014W.
NMPDR	fig|4932.3.peg.2269.
Organism-specific databases
CYGD	YFL014w.
SGD	S000001880. HSP12.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P22943.
Gene expression databases
GermOnline	YFL014W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016360. Heat_shock_protein_9/12_fun. IPR007250. HSP9_HSP12. [Graphical view]
Pfam	PF04119. HSP9_HSP12. 1 hit. [Graphical view]
PIRSF	PIRSF002590. HSP9/HSP12_fun. 1 hit.
ProDom	P22943. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P22943.
ProtoNet	Search...

Entry information

Entry name

HSP12_YEAST

Accession

Primary (citable) accession number: P22943
Secondary accession number(s): Q8X145

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	September 2, 2008
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)