Reviewed, UniProtKB/Swiss-Prot P23248 (RS3B_YEAST)

Names and origin

Protein names

Recommended name:
    40S ribosomal protein S1-B
Alternative name(s):
    RP10B

Gene names

Name:	RPS1B
Synonyms:	PLC2, RPS10B
Ordered Locus Names:	YML063W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).
Subcellular location	Cytoplasm.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA By similarity.
Miscellaneous	Present with 107000 molecules/cell in log phase SD medium. There are 2 genes for S1 in yeast.
Sequence similarities	Belongs to the ribosomal protein S3Ae family.
Caution	Was originally (Ref.1) thought to be MFT1, the mitochondrial fusion target protein.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Ref.5 Traceable author statement. Source: SGD
Cellular component	90S preribosome Inferred from direct assay. Source: SGD cytosolic small ribosomal subunit Ref.5 Traceable author statement. Source: SGD
Molecular function	structural constituent of ribosome Ref.5 Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 255	254	40S ribosomal protein S1-B
Amino acid modifications
	Modified residue	2	1	N-acetylalanine; partial By similarity
	Modified residue	76	1	Phosphoserine
	Modified residue	80	1	Phosphoserine
	Modified residue	154	1	Phosphoserine
	Modified residue	159	1	Phosphoserine
	Modified residue	245	1	Phosphoserine
	Modified residue	254	1	Phosphothreonine

Sequences

	Sequence		Length	Mass (Da)
	P23248-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 192A913E3AD5C7DE	FASTA	255	28,812
	10 20 30 40 50 60 MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKNASDA 70 80 90 100 110 120 LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF HGMDFTTDKL RSMVRKWQTL 130 140 150 160 170 180 IEANVTVKTS DDYVLRIFAI AFTRKQANQV KRHSYAQSSH IRAIRKVISE ILTREVQNST 190 200 210 220 230 240 LAQLTSKLIP EVINKEIENA TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK 250 GKKVSGFKDE VLETV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene." Garrett J.M., Singh K.K., Vonder Haar R.A., Emr S.D. Mol. Gen. Genet. 225:483-491(1991) [PubMed: 2017143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SEY2102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed: 1544921] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, MASS SPECTROMETRY.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, MASS SPECTROMETRY.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-154 AND SER-159, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55360 Genomic DNA. Translation: CAA39044.1. Z38114 Genomic DNA. Translation: CAA86258.1. AY557981 Genomic DNA. Translation: AAS56307.1.
PIR	S14051.
RefSeq	NP_013648.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P23248.
Proteomic databases
PeptideAtlas	P23248.
Genome annotation databases
Ensembl	YML063W. Saccharomyces cerevisiae. [Contig view]
GeneID	854939.
GenomeReviews	Gene locus YML063W in contig Z71257_GR.
KEGG	sce:YML063W.
NMPDR	fig|4932.3.peg.4685.
Organism-specific databases
CYGD	YML063w.
SGD	S000004528. RPS1B.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P23248.
Gene expression databases
ArrayExpress	P23248.
GermOnline	YML063W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001593. Ribosomal_S3Ae. [Graphical view]
PANTHER	PTHR11830. Ribosomal_S3AE. 1 hit.
Pfam	PF01015. Ribosomal_S3Ae. 1 hit. [Graphical view]
ProDom	PD003035. Ribosomal_S3AE. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01191. RIBOSOMAL_S3AE. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	P23248.
ProtoNet	Search...

Entry information

Entry name

RS3B_YEAST

Accession

Primary (citable) accession number: P23248

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	January 23, 2007
Last modified:	September 2, 2008
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)