Reviewed, UniProtKB/Swiss-Prot P23298 (KPCL_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein kinase C eta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-eta
    PKC-L

Gene names

Name:	Prkch
Synonyms:	Pkch

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation.
Tissue specificity	Predominantly expressed in lunk and skin.
Domain	The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 683	683	Protein kinase C eta type
Regions
	Domain	12 – 102	91	C2
	Domain	355 – 614	260	Protein kinase
	Domain	615 – 683	69	AGC-kinase C-terminal
	Zinc finger	171 – 222	52	Phorbol-ester/DAG-type 1
	Zinc finger	245 – 295	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	361 – 369	9	ATP By similarity
Sites
	Active site	479	1	Proton acceptor By similarity
	Binding site	384	1	ATP By similarity
Amino acid modifications
	Modified residue	513	1	Phosphothreonine Potential
	Modified residue	656	1	Phosphothreonine Probable
	Modified residue	675	1	Phosphoserine Probable

Sequences

	Sequence		Length	Mass (Da)
	P23298-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: C7DB338A9F95F576	FASTA	683	77,974
	10 20 30 40 50 60 MSSGTMKFNG YLRVRIGEAV GLQPTRWSLR HSLFKKGHQL LDPYLTVSVD QVRVGQTSTK 70 80 90 100 110 120 QKTNKPTYNE EFCANVTDGG HLELAVFHET PLGYDHFVAN CTLQFQELLR TAGTSDTFEG 130 140 150 160 170 180 WVDLEPEGKV FVVITLTGSF TEATLQRDRI FKHFTRKRQR AMRRRVHQVN GHKFMATYLR 190 200 210 220 230 240 QPTYCSHCRE FIWGVFGKQG YQCQVCTCVV HKRCHHLIVT ACTCQNNINK VDAKIAEQRF 250 260 270 280 290 300 GINIPHKFNV HNYKVPTFCD HCGSLLWGIM RQGLQCKICK MNVHIRCQAN VAPNCGVNAV 310 320 330 340 350 360 ELAKTLAGMG LQPGNISPTS KLISRSTLRR QGKEGSKEGN GIGVNSSSRF GIDNFEFIRV 370 380 390 400 410 420 LGKGSFGKVM LARIKETGEL YAVKVLKKDV ILQDDDVECT MTEKRILSLA RNHPFLTQLF 430 440 450 460 470 480 CCFQTPDRLF FVMEFVNGGD LMFHIQKSRR FDEARARFYA AEIISALMFL HEKGIIYRDL 490 500 510 520 530 540 KLDNVLLDHE GHCKLADFGM CKEGICNGVT TATFCGTPDY IAPEILQEML YGPAVDWWAM 550 560 570 580 590 600 GVLLYEMLCG HAPFEAENED DLFEAILNDE VVYPTWLHED ARGILKSFMT KNPTMRLGSL 610 620 630 640 650 660 TQGGEHEILR HPFFKEIDWA QLNHRQLEPP FRPRIKSRED VSNFDPDFIK EEPVLTPIDE 670 680 GHLPMINQDE FRNFSYVSPE LQL

References

[1]

"A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin." Osada S., Mizuno K., Saido T.C., Akita Y., Suzuki K., Kuroki T., Ohno S. J. Biol. Chem. 265:22434-22440(1990) [PubMed: 2266135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Epidermis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90242 mRNA. Translation: BAA14288.1.
PIR	A23690.
UniGene	Mm.341677
3D structure databases
HSSP	HSSP built from PDB template 1PTQ based on UniProtKB P28867.
SMR	P23298. Positions 2-137.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000021108. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:97600. Prkch.
Phylogenomic databases
HOGENOM	P23298.
HOVERGEN	P23298.
Gene expression databases
ArrayExpress	P23298.
CleanEx	MM_PRKCH.
GermOnline	ENSMUSG00000021108. Mus musculus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR002219. DAG_PE_bd. IPR015745. PKC. IPR000961. Pkinase_C. IPR014376. Prot_kin_PKC_delta. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000551. PKC_delta. 1 hit.
PRINTS	PR00360. C2DOMAIN. PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	P23298.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

KPCL_MOUSE

Accession

Primary (citable) accession number: P23298

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	July 22, 2008
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)