Reviewed, UniProtKB/Swiss-Prot P24546 (T2A1_ACICA)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme AccI
      Short name=R.AccI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme AccI
    Endonuclease AccI

Gene names

Name:

accIR

Organism

Acinetobacter calcoaceticus

Taxonomic identifier

471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GTMKAC and cleaves after T-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Subunit structure	Homotetramer.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 366	366	Type-2 restriction enzyme AccI

Sequences

	Sequence		Length	Mass (Da)
	P24546-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: DB663B74351C415E	FASTA	366	42,495
	10 20 30 40 50 60 MDYYDRIREL TKNVPVELVD FEQPRDLART PTQASSNFIT NKEQGDWAED LVTRAINENS 70 80 90 100 110 120 KNFVAVKYGK SDNLVAGENG FDTFYQDFQT ELDTIGKRPD LLIFKKTDFD TTLGFDVSQI 130 140 150 160 170 180 PHHQITDYVK KAIAGIEVRS SAFLIDKYEE AMQVRTQRFT EIAFQTRDKI LAEFLDVLDH 190 200 210 220 230 240 PSRSKYITLL NTLTLETISI FDFKVPGWRS NERLIEVNNL FKRLKVAIKE IQKRDYLSIT 250 260 270 280 290 300 PKVEDIKVVY KWIETFNVPH FYFQVFFDKV YGISFEQILT IISNSDNDGV IFSVEKDVQN 310 320 330 340 350 360 QNKTTIKINS KTGYPIASKV DEPTHESIRK EMDRGRLLFY VTFKGGTAYL DLDNLRTILG IEEAEF

References

[1]

"Cloning and nucleotide sequences of the AccI restriction-modification genes in Acinetobacter calcoaceticus." Kawakami B., Hilzheber C., Nagatomo M., Oka M. Agric. Biol. Chem. 55:1553-1559(1991) [PubMed: 1368703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: ATCC 49823.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10671 Genomic DNA. Translation: BAA01522.1.
PIR	JU0469.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	18. AccI.
Family and domain databases
ProDom	P24546. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

T2A1_ACICA

Accession

Primary (citable) accession number: P24546

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	September 2, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)