Reviewed, UniProtKB/Swiss-Prot P25217 (T2BF_BACSU)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme BsuFI
      Short name=R.BsuFI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme BsuFI
    Endonuclease BsuFI

Gene names

Name:	hsdFR
Synonyms:	hsrF

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCGG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 395	395	Type-2 restriction enzyme BsuFI

Sequences

	Sequence		Length	Mass (Da)
	P25217-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: C1E7D88FDAAC623D	FASTA	395	45,540
	10 20 30 40 50 60 MNKDNQIKNE SGKQAKILVS EIVNNLKNEL GINIEIEEGY SIGYPNQEKQ FKMDFLVQFT 70 80 90 100 110 120 DFDNEQWLIK STNSIRERIY GTEFFAQNIR LIDEKVKNIY VVVPDSISSA EMKKKRNYSV 130 140 150 160 170 180 KINGTTYTSF LTDVLTVNEL RQKIVEKASQ NIAQGLRANV LGNDAETSIV NLLNDLKNKA 190 200 210 220 230 240 LWNDYQNAQQ TIKSSTYKIY KEILEKIDLK EGFDKILEVT ATNDIPLLSN RGKPKTDVSV 250 260 270 280 290 300 TIKTNTKELI RNISIKNTRE KTVTIHEGSV SDLISRLKLS ETDPLSQALI HFEKVGSKKK 310 320 330 340 350 360 LIAEHPNSDK ILEENLKLYN RELIEFLHSP LLNDKIQMVD LIIFTNKFAV WNRDDYIKHY 370 380 390 IEEYSGKGQF GTPFKWTYPS KKRGQKIQIK GFSNN

References

[1]

"Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme." Kapfer W., Walter J., Trautner T.A. Nucleic Acids Res. 19:6457-6463(1991) [PubMed: 1721700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ISF18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62104 Genomic DNA. Translation: CAA44014.1.
PIR	S20059.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	618. BsuFI.
Family and domain databases
InterPro	IPR015291. Restrict_endonuc_II_MspI. [Graphical view]
Pfam	PF09208. Endonuc-MspI. 1 hit. [Graphical view]
ProDom	P25217. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

T2BF_BACSU

Accession

Primary (citable) accession number: P25217

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	July 22, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)