Inorganic phosphate transporter PHO84 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P25297 (PHO84_YEAST)

Last modified September 2, 2008. Version 78. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Inorganic phosphate transporter PHO84

Gene names

Name:	PHO84
Ordered Locus Names:	YML123C
ORF Names:	YM7056.03C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	High-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast.
Subunit structure	May function as a monomer.
Subcellular location	Membrane; Multi-pass membrane protein.
Induction	Under the control of phosphate. It is derepressed by phosphate starvation.
Miscellaneous	Present with 335000 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

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Ontologies

Keywords
Biological process	Phosphate transport Transport
Cellular component	Membrane
Domain	Transmembrane
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	manganese ion transport Inferred from mutant phenotype. Source: SGD phosphate transport Ref.1 Inferred from direct assay. Source: SGD polyphosphate metabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	integral to plasma membrane Ref.1 Inferred from direct assay. Source: SGD
Molecular function	inorganic phosphate transmembrane transporter activity Ref.1 Inferred from direct assay. Source: SGD manganese ion transmembrane transporter activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 587

587

Inorganic phosphate transporter PHO84

Regions

Topological domain

1 – 67

Cytoplasmic Potential

Transmembrane

68 – 88

1 Potential

Topological domain

89 – 108

Extracellular Potential

Transmembrane

109 – 129

2 Potential

Topological domain

130 – 133

Cytoplasmic Potential

Transmembrane

134 – 154

3 Potential

Topological domain

155 – 156

Extracellular Potential

Transmembrane

157 – 177

4 Potential

Topological domain

178 – 201

Cytoplasmic Potential

Transmembrane

202 – 222

5 Potential

Topological domain

223 – 250

Extracellular Potential

Transmembrane

251 – 271

6 Potential

Topological domain

272 – 345

Cytoplasmic Potential

Transmembrane

346 – 366

7 Potential

Topological domain

367 – 395

Extracellular Potential

Transmembrane

396 – 416

8 Potential

Topological domain

417 – 419

Cytoplasmic Potential

Transmembrane

420 – 440

9 Potential

Topological domain

441 – 442

Extracellular Potential

Transmembrane

443 – 463

10 Potential

Topological domain

464 – 485

Cytoplasmic Potential

Transmembrane

486 – 506

11 Potential

Topological domain

507 – 522

Extracellular Potential

Transmembrane

523 – 543

12 Potential

Topological domain

544 – 587

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphothreonine

Modified residue

302

Phosphothreonine

Modified residue

303

Phosphoserine

Modified residue

316

Phosphoserine

Modified residue

317

Phosphothreonine

Modified residue

321

Phosphoserine

Modified residue

577

Phosphoserine

Modified residue

578

Phosphoserine

Modified residue

579

Phosphoserine

Modified residue

581

Phosphoserine

Cross-link

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Cross-link

298

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Experimental info

Sequence conflict

162

A → AHSPAINFVA Ref.1

Sequence conflict

353

S → Y in BAA14358. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P25297-1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E463954395E8A840
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FASTA

587

64,382

        10         20         30         40         50         60 
MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL ESIDDEGFGW 

        70         80         90        100        110        120 
QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP GPSQTLLKVS TSVGTVIGQF 

       130        140        150        160        170        180 
GFGTLADIVG RKRIYGMELI IMIVCTILQT TVAHSPAINF VAVLTFYRIV MGIGIGGDYP 

       190        200        210        220        230        240 
LSSIITSEFA TTKWRGAIMG AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR 

       250        260        270        280        290        300 
CQKACDQMWR ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH 

       310        320        330        340        350        360 
DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT AGSWFTLDVA 

       370        380        390        400        410        420 
FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA GSLPGYWVSV FTVDIIGRKP 

       430        440        450        460        470        480 
IQLAGFIILT ALFCVIGFAY HKLGDHGLLA LYVICQFFQN FGPNTTTFIV PGECFPTRYR 

       490        500        510        520        530        540 
STAHGISAAS GKVGAIIAQT ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL 

       550        560        570        580 
LIPETKRKTL EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter." Bun-Ya M., Nishimura M., Harashima S., Oshima Y. Mol. Cell. Biol. 11:3229-3238(1991) [PubMed: 2038328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; THR-317; SER-321 AND SER-579, UBIQUITINATION AT LYS-6 AND LYS-298, MASS SPECTROMETRY.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; SER-316; THR-317; SER-321; SER-577; SER-579 AND SER-581, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-317; SER-321 AND SER-578, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-22; THR-302; SER-303; SER-316; THR-317; SER-321; SER-577; SER-578; SER-579 AND SER-581, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	D90346 Genomic DNA. Translation: BAA14358.1. Z49218 Genomic DNA. Translation: CAA89157.1.
PIR	S54061.
RefSeq	NP_013583.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5072N.
IntAct	P25297.
Proteomic databases
PeptideAtlas	P25297.
Genome annotation databases
Ensembl	YML123C. Saccharomyces cerevisiae. [Contig view]
GeneID	854916.
GenomeReviews	Gene locus YML123C in contig Z71257_GR.
KEGG	sce:YML123C.
NMPDR	fig\|4932.3.peg.4619.
Organism-specific databases
CYGD	YML123c.
SGD	S000004592. PHO84.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P25297.
Gene expression databases
GermOnline	YML123C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007114. MFS. IPR004738. Phos_permease. IPR005828. Sub_transporter. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00887. 2A0109. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 1 hit. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProDom	P25297. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P25297.
ProtoNet	Search...

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Entry information

Entry name

PHO84_YEAST

Accession

Primary (citable) accession number: P25297

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1996
Last modified:	September 2, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents