Reviewed, UniProtKB/Swiss-Prot P25651 (CSM1_YEAST)

Names and origin

Protein names

Recommended name:
    Monopolin complex subunit CSM1
Alternative name(s):
    Chromosome segregation in meiosis protein 1

Gene names

Name:	CSM1
Synonyms:	SPO86
Ordered Locus Names:	YCR086W
ORF Names:	YCR86W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Plays also a mitotic role in DNA replication.
Subunit structure	Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore. Interacts with CDC46, CDC47, GIC1, GIC2 and MCM3.
Subcellular location	Nucleus › nucleolus. Note= Transiently released from the nucleolus and localizes to the centromere regions during late pachytene.
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Meiosis
Cellular component	Nucleus
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Ref.8 Inferred from mutant phenotype. Source: SGD homologous chromosome segregation Ref.5 Inferred from mutant phenotype. Source: SGD response to drug Inferred from mutant phenotype. Source: SGD
Cellular component	monopolin complex Ref.5 Inferred from direct assay. Source: SGD nuclear envelope Ref.4 Inferred from direct assay. Source: SGD nucleolus Ref.5 Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.8 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q12066	1	EBI-22001,EBI-35877
CDC47	P38132	1	EBI-22001,EBI-4300
DSE3	Q08729	1	EBI-22001,EBI-32325
LRS4	Q04087	1	EBI-22001,EBI-32189
MAM1	P40065	1	EBI-22001,EBI-22643
MCM3	P24279	1	EBI-22001,EBI-10541
MCM5	P29496	1	EBI-22001,EBI-10549
PLP2	Q12017	1	EBI-22001,EBI-39032
SMC1	P32908	2	EBI-22001,EBI-17402

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Monopolin complex subunit CSM1		PRO_0000202577
Regions
	Coiled coil	31 – 70	40	Potential

Sequences

	Sequence		Length	Mass (Da)
	P25651-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 9650E0DA89B31CC5	FASTA	190	21,751
	10 20 30 40 50 60 MDPLTVYKNS VKQQIDSADL LVANLVNENF VLSEKLDTKA TEIKQLQKQI DSLNAQVKEL 70 80 90 100 110 120 KTQTSQQAEN SEVIKDLYEY LCNVRVHKSY EDDSGLWFDI SQGTHSGGSS DDYSIMDYKL 130 140 150 160 170 180 GFVKGQAQVT EVIYAPVLKQ RSTEELYSLQ SKLPEYLFET LSFPLSSLNQ FYNKIAKSLN 190 KKREKKDETE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A screen for genes required for meiosis and spore formation based on whole-genome expression." Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K. Curr. Biol. 11:1001-1009(2001) [PubMed: 11470404] [Abstract] Cited for: FUNCTION.
[4]	"A protein interaction map for cell polarity development." Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W., Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R., McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P., Boone C. , Pringle J.R., Davis T.N., Fields S., Drubin D.G. J. Cell Biol. 154:549-571(2001) [PubMed: 11489916] [Abstract] Cited for: INTERACTION WITH GIC1 AND GIC2, SUBCELLULAR LOCATION.
[5]	"Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I." Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K. Dev. Cell 4:535-548(2003) [PubMed: 12689592] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex." Wysocka M., Rytka J., Kurlandzka A. Exp. Cell Res. 294:592-602(2004) [PubMed: 15023545] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC46; CDC47 AND MCM3.
[9]	"A DNA integrity network in the yeast Saccharomyces cerevisiae." Pan X., Ye P., Yuan D.S., Wang X., Bader J.S., Boeke J.D. Cell 124:1069-1081(2006) [PubMed: 16487579] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59720 Genomic DNA. Translation: CAA42261.1. AY558153 Genomic DNA. Translation: AAS56479.1.
PIR	S19501.
RefSeq	NP_010009.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1283N.
IntAct	P25651.
Proteomic databases
PeptideAtlas	P25651.
Genome annotation databases
Ensembl	YCR086W. Saccharomyces cerevisiae. [Contig view]
GeneID	850447.
GenomeReviews	Gene locus YCR086W in contig X59720_GR.
KEGG	sce:YCR086W.
NMPDR	fig|4932.3.peg.738.
Organism-specific databases
CYGD	YCR086w.
SGD	S000000682. CSM1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P25651.
Gene expression databases
GermOnline	YCR086W. Saccharomyces cerevisiae.
Family and domain databases
BLOCKS	Search...
ProtoNet	Search...
Other Resources
LinkHub	P25651.
NextBio	966060.

Entry information

Entry name

CSM1_YEAST

Accession

Primary (citable) accession number: P25651

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	November 4, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)