Reviewed, UniProtKB/Swiss-Prot P25750 (CH60_BUCAI)

Names and origin

Protein names

Recommended name:
    60 kDa chaperonin
Alternative name(s):
    Protein Cpn60
    groEL protein
    Symbionin

Gene names

Name:	groL
Synonyms:	groEL, mopA, symL
Ordered Locus Names:	BU019

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions By similarity.
Subunit structure	Oligomer of 14 subunits composed of two stacked rings of 7 subunits By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the chaperonin (HSP60) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein refolding Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP unfolded protein binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 548	548	60 kDa chaperonin

Sequences

	Sequence		Length	Mass (Da)
	P25750-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 9B33AE1E629E7592	FASTA	548	57,911
	10 20 30 40 50 60 MAAKDVKFGN EARIKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPSIT KDGVSVAREI 70 80 90 100 110 120 ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATLLAQSIV NEGLKAVAAG MNPMDLKRGI 130 140 150 160 170 180 DKAVISAVEE LKHLSVPCSD SKAITQVGTI SANADEKVGS LIAEAMEKVG NDGVITVEEG 190 200 210 220 230 240 TGLQDELEVV KGMQFDRGYL SPYFINKPET GIVELENPYI LMADKKISNV REMLPILESV 250 260 270 280 290 300 AKSGKPLLII SEDLEGEALA TLVVNSMRGI VKVAAVKAPG FGDRRKAMLQ DISILTGGSV 310 320 330 340 350 360 ISEELAMELE KSTLEDLGQA KRVVISKDTT TIIGGVGEKH SIQSRISQIR QEIQEATSDY 370 380 390 400 410 420 DKEKLNERLA KLSGGVAVLK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALV 430 440 450 460 470 480 RVAGKIADLR GQNEDQNVGI RVALRAMEAP LRQIVSNSGE EPSVVTNNVK DGKGNYGYNA 490 500 510 520 530 540 ATDEYGDMID FGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKEDKS SDSSSSPAGG MGGMGGMM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures of chaperonins from an intracellular symbiont and their functional expression in Escherichia coli groE mutants." Ohtaka C., Nakamura H., Ishikawa H. J. Bacteriol. 174:1869-1874(1992) [PubMed: 1347769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61150 Genomic DNA. Translation: CAA43460.1. BA000003 Genomic DNA. Translation: BAB12746.1.
RefSeq	NP_239860.1.
3D structure databases
HSSP	HSSP built from PDB template 1GR5 based on UniProtKB P06139.
SMR	P25750. Positions 2-526.
ModBase	Search...
Genome annotation databases
GeneID	1109527.
GenomeReviews	Gene locus BU019 in contig BA000003_GR.
KEGG	buc:BU019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P25750.
Enzyme and pathway databases
BioCyc	BSP107806:BU019-MON.
Family and domain databases
HAMAP	MF_00600. [Tree]
InterPro	IPR001844. Chaprnin_Cpn60. IPR002423. Cpn60/TCP-1. IPR012723. GroEL. [Graphical view]
PANTHER	PTHR11353. Cpn60/TCP-1. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00298. CHAPERONIN60. PR00304. TCOMPLEXTCP1.
TIGRFAMs	TIGR02348. GroEL. 1 hit.
PROSITE	PS00296. CHAPERONINS_CPN60. 1 hit. [Graphical view]
ProDom	P25750. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CH60_BUCAI

Accession

Primary (citable) accession number: P25750

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	July 22, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)