Reviewed, UniProtKB/Swiss-Prot P26919 (T2A1_AZOBR)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme AbrI
      Short name=R.AbrI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme AbrI
    Endonuclease AbrI

Gene names

Name:

abrIR

Organism

Azospirillum brasilense

Taxonomic identifier

192 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 222	222	Type-2 restriction enzyme AbrI
Regions
	Compositional bias	41 – 47	7	Arg/Lys-rich (basic)

Sequences

	Sequence		Length	Mass (Da)
	P26919-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 8FB124EFC4BBDCC1	FASTA	222	25,260
	10 20 30 40 50 60 MALELADYER KARESVMAFW GNREKARQKQ QESGKPDQGE RRRDRRKNMD GFCALVIDII 70 80 90 100 110 120 RANGLHRAEI HQKRALLTLP GYFRPTKLWD LLVIQDRRLV AALEFKSQVG PSFGNNFNNR 130 140 150 160 170 180 TEEAIGTAHD LWTAYREGAF GQSPRPFVGW LMLVEDAPES NQRRADTSPH FRCSRSSRAH 190 200 210 220 PTYGGMTSSA SGSSRSSFTP RPRSSPRRAP PPRQGRIPSP RI

References

[1]	"AbrI restriction modification system from Azospirillium brasilense, molecular cloning and characterization of its genes." Schwabe G., Helke A., Klingmueller W. Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29711 / DSM 1844.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62690 Genomic DNA. Translation: CAA44567.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	8. AbrI.
Family and domain databases
InterPro	IPR007636. Restrict_endonuc_II_XhoI. [Graphical view]
Pfam	PF04555. XhoI. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

T2A1_AZOBR

Accession

Primary (citable) accession number: P26919

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	July 22, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)