Reviewed, UniProtKB/Swiss-Prot P27115 (MGAT1_RABIT)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
    EC=2.4.1.101
Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
      Short name=GlcNAc-T I
      Short name=GNT-I

Gene names

Name:	MGAT1
Synonyms:	GNT1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Appears to be present in all tissues.
Sequence similarities	Belongs to the glycosyltransferase 13 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 447

447

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

PRO_0000191387

Regions

Topological domain

1 – 6

6

Cytoplasmic Potential

Transmembrane

7 – 29

23

Signal-anchor for type II membrane protein Potential

Topological domain

30 – 447

418

Lumenal Potential

Secondary structure

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447

Helix Strand Turn

Details...

Beta strand

110 – 116

7

Helix

120 – 130

11

Turn

134 – 136

3

Beta strand

139 – 143

5

Helix

148 – 155

8

Helix

156 – 160

5

Beta strand

161 – 165

5

Helix

179 – 181

3

Helix

182 – 199

18

Beta strand

205 – 211

7

Beta strand

214 – 216

3

Helix

220 – 233

14

Beta strand

237 – 242

6

Helix

249 – 251

3

Beta strand

260 – 265

6

Beta strand

269 – 274

6

Helix

275 – 281

7

Helix

282 – 284

3

Helix

290 – 294

5

Helix

297 – 300

4

Beta strand

304 – 315

12

Helix

343 – 345

3

Helix

349 – 351

3

Helix

353 – 366

14

Helix

372 – 376

5

Beta strand

384 – 389

6

Helix

393 – 402

10

Beta strand

421 – 426

6

Beta strand

429 – 434

6

Sequences

	Sequence		Length	Mass (Da)
	P27115-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 18AC90994F0EB435	FASTA	447	51,540
	10 20 30 40 50 60 MLKKQSAGLV LWGAILFVAW NALLLLFFWT RPVPSRLPSD NALDDDPASL TREVIRLAQD 70 80 90 100 110 120 AEVELERQRG LLQQIREHHA LWSQRWKVPT AAPPAQPHVP VTPPPAVIPI LVIACDRSTV 130 140 150 160 170 180 RRCLDKLLHY RPSAELFPII VSQDCGHEET AQVIASYGSA VTHIRQPDLS NIAVQPDHRK 190 200 210 220 230 240 FQGYYKIARH YRWALGQIFH NFNYPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV 250 260 270 280 290 300 SAWNDNGKEQ MVDSSKPELL YRTDFFPGLG WLLLAELWAE LEPKWPKAFW DDWMRRPEQR 310 320 330 340 350 360 KGRACVRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY LQQEAYDRDF 370 380 390 400 410 420 LARVYGAPQL QVEKVRTNDR KELGEVRVQY TGRDSFKAFA KALGVMDDLK SGVPRAGYRG 430 440 IVTFLFRGRR VHLAPPQTWD GYDPSWT

References

[1]

"Molecular cloning and expression of cDNA encoding the enzyme that controls conversion of high-mannose to hybrid and complex N-glycans: UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I." Sarkar M., Hull E., Nishikawa Y., Simpson R.J., Moritz R.L., Dunn R., Schachter H. Proc. Natl. Acad. Sci. U.S.A. 88:234-238(1991) [PubMed: 1824724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M57301 mRNA. Translation: AAA31493.1.

PIR

A38561.

RefSeq

NP_001076214.1.

UniGene

Ocu.1975

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FO8	X-ray	1.40	A	106-447	[»]
1FO9	X-ray	1.50	A	106-447	[»]
1FOA	X-ray	1.80	A	106-447	[»]
2AM3	X-ray	1.80	A	106-447	[»]
2AM4	X-ray	1.70	A	106-447	[»]
2AM5	X-ray	1.60	A	106-447	[»]
2APC	X-ray	1.50	A	106-447	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSOCUG00000006544. Oryctolagus cuniculus. [Contig view]

GeneID

100009521.

Phylogenomic databases

HOVERGEN

P27115.

Family and domain databases

InterPro

IPR004139. Glyco_trans_13.
[Graphical view]

PANTHER

PTHR10468. Glyco_trans_13. 1 hit.

Pfam

PF03071. GNT-I. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MGAT1_RABIT

Accession

Primary (citable) accession number: P27115

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)