Reviewed, UniProtKB/Swiss-Prot P28184 (MT3_MOUSE)

Names and origin

Protein names

Recommended name:
    Metallothionein-3
      Short name=MT-3
Alternative name(s):
    Metallothionein-III
      Short name=MT-III
    Growth inhibitory factor
      Short name=GIF

Gene names

Name:

Mt3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	68 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro By similarity.
Tissue specificity	Brain.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cellular metal ion homeostasis Inferred from direct assay. Source: MGI negative regulation of neurogenesis Inferred from direct assay. Source: MGI removal of superoxide radicals Inferred from sequence or structural similarity. Source: UniProtKB response to hypoxia Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	synaptic vesicle Inferred from direct assay. Source: UniProtKB
Molecular function	zinc ion binding Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 68

68

Metallothionein-3

Regions

Region

1 – 30

30

Beta

Region

31 – 68

38

Alpha

Sites

Metal binding

6

1

Cluster B By similarity

Metal binding

8

1

Cluster B By similarity

Metal binding

14

1

Cluster B By similarity

Metal binding

16

1

Cluster B By similarity

Metal binding

20

1

Cluster B By similarity

Metal binding

22

1

Cluster B By similarity

Metal binding

25

1

Cluster B By similarity

Metal binding

27

1

Cluster B By similarity

Metal binding

30

1

Cluster B By similarity

Metal binding

34

1

Cluster A By similarity

Metal binding

35

1

Cluster A By similarity

Metal binding

37

1

Cluster A By similarity

Metal binding

38

1

Cluster A By similarity

Metal binding

42

1

Cluster A By similarity

Metal binding

45

1

Cluster A By similarity

Metal binding

49

1

Cluster A By similarity

Metal binding

51

1

Cluster A By similarity

Metal binding

64

1

Cluster A By similarity

Metal binding

66

1

Cluster A By similarity

Metal binding

67

1

Cluster A By similarity

Amino acid modifications

Modified residue

1

1

N-acetylmethionine By similarity

Secondary structure

1

.

.

.

.

.

68

Helix Strand Turn

Details...

Beta strand

36 – 38

3

Turn

43 – 48

6

Sequences

	Sequence		Length	Mass (Da)
	P28184-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 791AF60E38FED3CA	FASTA	68	7,009
	10 20 30 40 50 60 MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV CKGEEGAKAE AEKCSCCQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MT-III, a brain-specific member of the metallothionein gene family." Palmiter R.D., Findley S.D., Whitmore T.E., Durnam D.M. Proc. Natl. Acad. Sci. U.S.A. 89:6333-6337(1992) [PubMed: 1631128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain.
[2]	"Structures of the human and mouse growth inhibitory factor-encoding genes." Naruse S., Igarashi S., Furuya T., Kobayashi H., Miyatake T., Tsuji S. Gene 144:283-287(1994) [PubMed: 8039715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3." Oz G., Zangger K., Armitage I.M. Biochemistry 40:11433-11441(2001) [PubMed: 11560491] [Abstract] Cited for: STRUCTURE BY NMR OF 32-68.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M93310 Genomic DNA. Translation: AAA39529.1.
S72046 Genomic DNA. Translation: AAB31397.1.
BC059725 mRNA. Translation: AAH59725.1.

PIR

A46034.

RefSeq

NP_038631.1.

UniGene

Mm.2064

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JI9	NMR	-	A	32-68	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSMUSG00000031760. Mus musculus. [Contig view]

GeneID

17751.

KEGG

mmu:17751.

Organism-specific databases

MGI

MGI:97173. Mt3.

Phylogenomic databases

HOGENOM

P28184.

HOVERGEN

P28184.

Gene expression databases

ArrayExpress

P28184.

CleanEx

MM_MT3.

GermOnline

ENSMUSG00000031760. Mus musculus.

Family and domain databases

InterPro

IPR015874. 4-disulphide_core.
IPR001450. 4Fe4S_Fe_S_bd.
IPR003019. Metallothionein_sfam_euk.
IPR000006. Metallothionein_vert.
[Graphical view]

Gene3D

G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.

PANTHER

PTHR23299. Metallothionein_vert. 1 hit.

Pfam

PF00131. Metallothio. 1 hit.
[Graphical view]

PRINTS

PR00003. 4DISULPHCORE.
PR00353. 4FE4SFRDOXIN.
PR00860. MTVERTEBRATE.

PROSITE

PS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

ProDom

P28184.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

MT3_MOUSE

Accession

Primary (citable) accession number: P28184

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	July 22, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)