Reviewed, UniProtKB/Swiss-Prot P28190 (AA1R_BOVIN)

Names and origin

Protein names

Recommended name:
    Adenosine receptor A1

Gene names

Name:

ADORA1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 326	326	Adenosine receptor A1
Regions
	Topological domain	1 – 10	10	Extracellular Potential
	Transmembrane	11 – 33	23	1 Potential
	Topological domain	34 – 46	13	Cytoplasmic Potential
	Transmembrane	47 – 69	23	2 Potential
	Topological domain	70 – 80	11	Extracellular Potential
	Transmembrane	81 – 102	22	3 Potential
	Topological domain	103 – 123	21	Cytoplasmic Potential
	Transmembrane	124 – 146	23	4 Potential
	Topological domain	147 – 176	30	Extracellular Potential
	Transmembrane	177 – 201	25	5 Potential
	Topological domain	202 – 235	34	Cytoplasmic Potential
	Transmembrane	236 – 259	24	6 Potential
	Topological domain	260 – 267	8	Extracellular Potential
	Transmembrane	268 – 292	25	7 Potential
	Topological domain	293 – 326	34	Cytoplasmic Potential
Amino acid modifications
	Lipidation	309	1	S-palmitoyl cysteine Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Disulfide bond	80 ↔ 169		By similarity
Experimental info
	Sequence conflict	212	1	N → S in CAA45135. Ref.1
	Sequence conflict	212	1	N → S in AAA30350. Ref.2
	Sequence conflict	315	1	V → I in CAA45135. Ref.1
	Sequence conflict	315	1	V → I in AAA30350. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P28190-1 [UniParc]. Last modified November 13, 2007. Version 3. Checksum: 6F4557FCEFEBC66E	FASTA	326	36,593
	10 20 30 40 50 60 MPPSISAFQA AYIGIEVLIA LVSVPGNVLV IWAVKVNQAL RDATFCFIVS LAVADVAVGA 70 80 90 100 110 120 LVIPLAILIN IGPRTYFHTC LKVACPVLIL TQSSILALLA IAVDRYLRVK IPLRYKTVVT 130 140 150 160 170 180 PRRAVVAITG CWILSFVVGL TPMFGWNNLS AVERDWLANG SVGEPVIECQ FEKVISMEYM 190 200 210 220 230 240 VYFNFFVWVL PPLLLMVLIY MEVFYLIRKQ LNKKVSASSG DPQKYYGKEL KIAKSLALIL 250 260 270 280 290 300 FLFALSWLPL HILNCITLFC PSCHMPRILI YIAIFLSHGN SAMNPIVYAF RIQKFRVTFL 310 320 KIWNDHFRCQ PAPPVDEDAP AERPDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a bovine adenosine A1 receptor cDNA." Tucker A.L., Linden J., Robeva A.S., D'Angelo D.D., Lynch K.R. FEBS Lett. 297:107-111(1992) [PubMed: 1551415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Cloning, expression, and characterization of the unique bovine A1 adenosine receptor. Studies on the ligand binding site by site-directed mutagenesis." Olah M.E., Ren H., Ostrowski J., Jacobson K., Stiles G.L. J. Biol. Chem. 267:10764-10770(1992) [PubMed: 1587851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pons and Hippocampus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63592 mRNA. Translation: CAA45135.1. M86261 mRNA. Translation: AAA30350.1. BT021759 mRNA. Translation: AAX46606.1. BC142208 mRNA. Translation: AAI42209.1. BC142265 mRNA. Translation: AAI42266.1.
PIR	A38144.
RefSeq	NP_776922.2.
UniGene	Bt.76442
3D structure databases
HSSP	HSSP built from PDB template 1MMH based on UniProtKB P29274.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSBTAG00000011461. Bos taurus. [Contig view]
GeneID	282133.
KEGG	bta:282133.
Phylogenomic databases
HOVERGEN	P28190.
Family and domain databases
InterPro	IPR001068. Adeno_A1_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR19266:SF15. Adeno_A1_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00552. ADENOSINEA1R. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P28190. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AA1R_BOVIN

Accession

Primary (citable) accession number: P28190
Secondary accession number(s): A5PJR1, Q58D38

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	November 13, 2007
Last modified:	September 2, 2008
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)