Reviewed, UniProtKB/Swiss-Prot P28263 (UBC8_YEAST)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2-24 kDa
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase
    Ubiquitin carrier protein
    Glucose-induced degradation protein 3

Gene names

Name:	UBC8
Synonyms:	GID3
Ordered Locus Names:	YEL012W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteosome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase).
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of gluconeogenesis Ref.5 Inferred from mutant phenotype. Source: SGD proteasomal ubiquitin-dependent protein catabolic process Ref.5 Inferred from mutant phenotype. Source: SGD protein monoubiquitination Traceable author statement. Source: SGD protein polyubiquitination Traceable author statement. Source: SGD protein ubiquitination during ubiquitin-dependent protein catabolic process Ref.4 Inferred from mutant phenotype. Source: SGD regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.4 Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Ref.1 Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-19755,EBI-19755

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Ubiquitin-conjugating enzyme E2-24 kDa		PRO_0000082555
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28263-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 24FFAD8B4679CE6F	FASTA	218	24,633
	10 20 30 40 50 60 MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR LHVELPDNYP 70 80 90 100 110 120 YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI NIVEWMIPGL LKEPNGSDPL 130 140 150 160 170 180 NNEAATLQLR DKKLYEEKIK EYIDKYATKE KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE 190 200 210 DMDGTGVSSG DDSVDELSED LSDIDVSDDD DYDEVANQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family." Qin S., Nakajima B., Nomura M., Arfin S.M. J. Biol. Chem. 266:15549-15554(1991) [PubMed: 1869573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"A human ubiquitin-conjugating enzyme homologous to yeast UBC8." Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R. J. Biol. Chem. 269:8797-8802(1994) [PubMed: 8132613] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS.
[4]	"Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast." Schuele T., Rose M., Entian K.-D., Thumm M., Wolf D.H. EMBO J. 19:2161-2167(2000) [PubMed: 10811607] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways." Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H. Mol. Biol. Cell 14:1652-1663(2003) [PubMed: 12686616] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M65083 Genomic DNA. No translation available. U18530 Genomic DNA. Translation: AAB64489.1. Different initiation. Z29329 Genomic DNA. Translation: CAA82526.1.
PIR	B53516.
RefSeq	NP_010904.2.
3D structure databases
HSSP	HSSP built from PDB template 1JBB based on UniProtKB P52490.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5299N.
IntAct	P28263.
Proteomic databases
PeptideAtlas	P28263.
Genome annotation databases
Ensembl	YEL012W. Saccharomyces cerevisiae. [Contig view]
GeneID	856704.
GenomeReviews	Gene locus YEL012W in contig U00092_GR.
KEGG	sce:YEL012W.
NMPDR	fig|4932.3.peg.1960.
Organism-specific databases
CYGD	YEL012w.
SGD	S000000738. UBC8.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P28263.
Gene expression databases
GermOnline	YEL012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. IPR015581. Ubq_conj_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621. UBQ-conjugat_E2. 1 hit. PTHR11621:SF19. Ubq_conj_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P28263.
NextBio	982771.

Entry information

Entry name

UBC8_YEAST

Accession

Primary (citable) accession number: P28263

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 1, 1994
Last modified:	November 25, 2008
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)