Reviewed, UniProtKB/Swiss-Prot P28906 (CD34_HUMAN)

Names and origin

Protein names

Recommended name:
    Hematopoietic progenitor cell antigen CD34
Alternative name(s):
    CD_antigen=CD34

Gene names

Name:

CD34

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Developmental stage	On early hematopoietic progenitor cells.
Post-translational modification	Highly glycosylated. Phosphorylated on serine residues by PKC.
Involvement in disease	Abnormal CD34 expression in leukemogenesis.
Sequence similarities	Belongs to the CD34 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
PTM	Glycoprotein Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell-cell adhesion Inferred from direct assay. Source: HGNC leukocyte migration Inferred from sequence or structural similarity. Source: HGNC
Cellular component	external side of plasma membrane Inferred by curator. Source: HGNC
Molecular function	carbohydrate binding Inferred from direct assay. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Notes: Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation.

Isoform CD34-F (identifier: P28906-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CD34-T (identifier: P28906-2)

The sequence of this isoform differs from the canonical sequence as follows:
     325-328: GEDP → ELEP
     329-385: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 31	31
	Chain	32 – 385	354	Hematopoietic progenitor cell antigen CD34
Regions
	Topological domain	32 – 290	259	Extracellular Potential
	Transmembrane	291 – 311	21	Potential
	Topological domain	312 – 385	74	Cytoplasmic Potential
Amino acid modifications
	Modified residue	329	1	Phosphotyrosine By similarity
	Modified residue	339	1	Phosphotyrosine By similarity
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	325 – 328	4	GEDP → ELEP in isoform CD34-T.
	Alternative sequence	329 – 385	57	Missing in isoform CD34-T.
Experimental info
	Sequence conflict	187	1	Q → E AA sequence Ref.7
	Sequence conflict	325 – 327	3	GED → ELE Ref.3
	Sequence conflict	361	1	E → K Ref.1 Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform CD34-F [UniParc]. Last modified July 15, 1998. Version 2. Checksum: BC581A8FCFFE2BB3	FASTA	385	40,716
	10 20 30 40 50 60 MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS NVSTNVSYQE 70 80 90 100 110 120 TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV 130 140 150 160 170 180 FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI 190 200 210 220 230 240 REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP 250 260 270 280 290 300 QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL 310 320 330 340 350 360 LAVLGITGYF LMNRRSWSPT GERLGEDPYY TENGGGQGYS SGPGTSPEAQ GKASVNRGAQ 370 380 ENGTGQATSR NGHSARQHVV ADTEL
	Isoform CD34-T [UniParc]. Checksum: 7FFBEBC98D4CC61D		328	35,011
	10 20 30 40 50 60 MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS NVSTNVSYQE 70 80 90 100 110 120 TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV 130 140 150 160 170 180 FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI 190 200 210 220 230 240 REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP 250 260 270 280 290 300 QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL 310 320 LAVLGITGYF LMNRRSWSPT GERLELEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells." Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B. J. Immunol. 148:267-271(1992) [PubMed: 1370171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CD34-F), PROTEIN SEQUENCE OF 32-43. Tissue: Bone marrow.
[2]	"Structure of the gene encoding CD34, a human hematopoietic stem cell antigen." Satterthwaite A.B., Burn T.C., le Beau M.M., Tenen D.G. Genomics 12:788-794(1992) [PubMed: 1374051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM CD34-F).
[3]	"Two alternative forms of cDNA encoding CD34." Nakamura Y., Komano H., Nakauchi H. Exp. Hematol. 21:236-242(1993) [PubMed: 7678811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CD34-F AND CD34-T). Tissue: Megakaryoblast.
[4]	"Sequence analysis of the human CD34 antigen." Freund D., Wiebe G.J., Ehninger G., Corbeil D. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CD34-F). Tissue: Heart.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CD34-F). Tissue: Skin.
[7]	"Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34." Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A., Greaves M.F., Smart J.E. Leukemia 2:793-803(1988) [PubMed: 2462139] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-47 AND 175-191, CHARACTERIZATION.
[8]	"Activated protein kinase C directly phosphorylates the CD34 antigen on hematopoietic cells." Fackler M.J., Civin C.I., Sutherland D.R., Baker M.A., May W.S. J. Biol. Chem. 265:11056-11061(1990) [PubMed: 1694174] [Abstract] Cited for: PHOSPHORYLATION BY PKC.
+	Additional computationally mapped references.

Web resources

Wikipedia

CD34 entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	M81104 mRNA. Translation: AAA03181.1. Different initiation. M81945 , M81938, M81939, M81940, M81941, M81942, M81943, M81944 Genomic DNA. Translation: AAA03659.1. S53910 mRNA. Translation: AAB25222.1. S53911 mRNA. Translation: AAB25223.1. AF523361 mRNA. Translation: AAM82157.1. AL356275 Genomic DNA. Translation: CAI40195.1. BC039146 mRNA. Translation: AAH39146.2.
PIR	A38078. I67604.
RefSeq	NP_001020280.1. NP_001764.1.
UniGene	Hs.374990
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P28906.
Genome annotation databases
Ensembl	ENSG00000174059. Homo sapiens. [Contig view]
GeneID	947.
KEGG	hsa:947.
Organism-specific databases
H-InvDB	HIX0001543.
HGNC	HGNC:1662. CD34.
HPA	CAB000018.
MIM	142230. gene.
PharmGKB	PA26211.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P28906.
HOVERGEN	P28906.
Enzyme and pathway databases
Reactome	REACT_6900. Signaling in Immune System.
Gene expression databases
ArrayExpress	P28906.
CleanEx	HS_CD34.
GermOnline	ENSG00000174059. Homo sapiens.
Family and domain databases
InterPro	IPR008083. CD34. IPR013836. CD34/Podocalyxin. [Graphical view]
PANTHER	PTHR16677. CD34. 1 hit.
Pfam	PF06365. CD34_antigen. 1 hit. [Graphical view]
PRINTS	PR01700. CD34ANTIGEN.
ProDom	P28906. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

CD34_HUMAN

Accession

Primary (citable) accession number: P28906
Secondary accession number(s): Q15970 , Q15971, Q5JTA4, Q9UJB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 15, 1998
Last modified:	July 22, 2008
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)