Reviewed, UniProtKB/Swiss-Prot P29310 (1433Z_DROME)

Names and origin

Protein names

Recommended name:
    14-3-3 protein zeta
Alternative name(s):
    14-3-3-like protein
    Protein Leonardo

Gene names

Name:	14-3-3zeta
Synonyms:	14-3-3, 14-3-3EZ, leo, THAP
ORF Names:	CG17870

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required in Raf-dependent cell proliferation and photoreceptor differentiation during eye development. Acts upstream of Raf and downstream of Ras, and is essential for viability. Acts as a negative regulator by decreasing its voltage sensitivity.
Subunit structure	Homodimer; homodimerization is not essential for modulating the activity of Slo. Interacts with phosphorylated Slob; the interaction with Slob mediates an indirect interaction with Slo.
Subcellular location	CytoplasmBy similarity.
Tissue specificity	Predominantly expressed in the ventral nerve cord of the embryo, and in the neural tissues of the head. Also found in the region posterior to the morphogenetic furrow of the eye imaginal disk where cells differentiate as photoreceptors.
Developmental stage	Expressed throughout all stages of embryonic and larval development.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromosome segregation Inferred from mutant phenotype. Source: FlyBase germarium-derived oocyte fate determination Inferred from genetic interaction. Source: FlyBase mitotic cell cycle, embryonic Inferred from mutant phenotype. Source: FlyBase olfactory learning Non-traceable author statement. Source: FlyBase oocyte microtubule cytoskeleton polarization Inferred from genetic interaction. Source: FlyBase
Cellular component	germline ring canal Inferred from direct assay. Source: FlyBase nucleus Non-traceable author statement. Source: FlyBase
Molecular function	protein binding Ref.6 Inferred from physical interaction. Source: UniProtKB protein kinase C inhibitor activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VKK3	1	EBI-198120,EBI-160025
HDAC4	Q9VYF3	1	EBI-198120,EBI-149766
mei-P22	Q9VS36	1	EBI-198120,EBI-102811
phyl	Q27934	1	EBI-198120,EBI-77033
Slob	Q8IPH9	1	EBI-198100,EBI-142379
Tektin-A	Q9V3M9	1	EBI-198120,EBI-116269
Tfb2	Q9VUR1	1	EBI-198120,EBI-132746
yki	Q95TU5	1	EBI-198100,EBI-141254

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform VI (identifier: P29310-1) Also known as: D; J; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform VI' (identifier: P29310-2) Also known as: A; G; H; I; The sequence of this isoform differs from the canonical sequence as follows:      149-161: QTAYQDAFDISKG → KNAYQEAFDIAKT
Isoform C (identifier: P29310-3) The sequence of this isoform differs from the canonical sequence as follows:      146-161: DDSQTAYQDAFDISKG → EDSKKAYQEAFDIAKT      185-193: LNSPDKACQ → INSPARACH
Notes: No experimental confirmation available.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view
	Sequence conflict	149	1	K → E in AAN71617. Ref.5

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 248	248	14-3-3 protein zeta
Natural variations
	Alternative sequence	146 – 161	16	DDSQT…DISKG → EDSKKAYQEAFDIAKT in isoform C.
	Alternative sequence	149 – 161	13	QTAYQ…DISKG → KNAYQEAFDIAKT in isoform VI'.
	Alternative sequence	185 – 193	9	LNSPDKACQ → INSPARACH in isoform C.
Experimental info
	Mutagenesis	59	1	R → A: Abolishes homodimerization but not regulatory function on Slo; when associated with A-63
	Mutagenesis	63	1	R → A: Abolishes homodimerization but not regulatory function on Slo; when associated with A-59

Sequences

	Sequence		Length	Mass (Da)
	Isoform VI (D) (J) [UniParc]. Last modified December 1, 1992. Version 1. Checksum: C645CF3B10C6701A	FASTA	248	28,228
	10 20 30 40 50 60 MSTVDKEELV QKAKLAEQSE RYDDMAQAMK SVTETGVELS NEERNLLSVA YKNVVGARRS 70 80 90 100 110 120 SWRVISSIEQ KTEASARKQQ LAREYRERVE KELREICYEV LGLLDKYLIP KASNPESKVF 130 140 150 160 170 180 YLKMKGDYYR YLAEVATGDA RNTVVDDSQT AYQDAFDISK GKMQPTHPIR LGLALNFSVF 190 200 210 220 230 240 YYEILNSPDK ACQLAKQAFD DAIAELDTLN EDSYKDSTLI MQLLRDNLTL WTSDTQGDEA EPQEGGDN
	Isoform VI' (A) (G) (H) (I) [UniParc]. Checksum: 0A1C1005D62C9699		248	28,283
	10 20 30 40 50 60 MSTVDKEELV QKAKLAEQSE RYDDMAQAMK SVTETGVELS NEERNLLSVA YKNVVGARRS 70 80 90 100 110 120 SWRVISSIEQ KTEASARKQQ LAREYRERVE KELREICYEV LGLLDKYLIP KASNPESKVF 130 140 150 160 170 180 YLKMKGDYYR YLAEVATGDA RNTVVDDSKN AYQEAFDIAK TKMQPTHPIR LGLALNFSVF 190 200 210 220 230 240 YYEILNSPDK ACQLAKQAFD DAIAELDTLN EDSYKDSTLI MQLLRDNLTL WTSDTQGDEA EPQEGGDN
	Isoform C [UniParc]. Checksum: 307C55638427FB57		248	28,304
	10 20 30 40 50 60 MSTVDKEELV QKAKLAEQSE RYDDMAQAMK SVTETGVELS NEERNLLSVA YKNVVGARRS 70 80 90 100 110 120 SWRVISSIEQ KTEASARKQQ LAREYRERVE KELREICYEV LGLLDKYLIP KASNPESKVF 130 140 150 160 170 180 YLKMKGDYYR YLAEVATGDA RNTVVEDSKK AYQEAFDIAK TKMQPTHPIR LGLALNFSVF 190 200 210 220 230 240 YYEIINSPAR ACHLAKQAFD DAIAELDTLN EDSYKDSTLI MQLLRDNLTL WTSDTQGDEA EPQEGGDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a Drosophila melanogaster gene similar to the mammalian genes encoding the tyrosine/tryptophan hydroxylase activator and protein kinase C inhibitor proteins." Swanson K.D., Ganguly R. Gene 113:183-190(1992) [PubMed: 1349290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VI). Strain: Oregon-R. Tissue: Head.
[2]	"Requirement for Drosophila 14-3-3 zeta in Raf-dependent photoreceptor development." Kockel L., Vorbrueggen G., Jaeckle H., Mlodzik M., Bohmann D. Genes Dev. 11:1140-1147(1997) [PubMed: 9159395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS VI AND VI'). Strain: Oregon-R.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: Berkeley. Tissue: Head.
[6]	"A dynamically regulated 14-3-3, Slob, and Slowpoke potassium channel complex in Drosophila presynaptic nerve terminals." Zhou Y., Schopperle W.M., Murrey H., Jaramillo A., Dagan D., Griffith L.C., Levitan I.B. Neuron 22:809-818(1999) [PubMed: 10230800] [Abstract] Cited for: FUNCTION, INTERACTION WITH SLOB.
[7]	"Monomeric 14-3-3 protein is sufficient to modulate the activity of the Drosophila slowpoke calcium-dependent potassium channel." Zhou Y., Reddy S., Murrey H., Fei H., Levitan I.B. J. Biol. Chem. 278:10073-10080(2003) [PubMed: 12529354] [Abstract] Cited for: HOMODIMERIZATION, MUTAGENESIS OF ARG-59 AND ARG-63.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M77518 mRNA. Translation: AAA28324.1. Y12573 Genomic DNA. Translation: CAA73152.1. Y12573 Genomic DNA. Translation: CAA73153.1. AE013599 Genomic DNA. Translation: AAF58842.4. AE013599 Genomic DNA. Translation: AAF58843.3. AE013599 Genomic DNA. Translation: AAM71060.1. AE013599 Genomic DNA. Translation: AAM71063.2. AE013599 Genomic DNA. Translation: AAS64884.1. AE013599 Genomic DNA. Translation: AAX52715.1. AE013599 Genomic DNA. Translation: AAX52716.1. BT001855 mRNA. Translation: AAN71617.1.
PIR	JC1122.
RefSeq	NP_001014515.1. NP_001014516.1. NP_476885.2. NP_724884.1. NP_724885.1. NP_724887.2. NP_724889.2. NP_995792.1.
UniGene	Dm.4705
3D structure databases
HSSP	HSSP built from PDB template 1O9E based on UniProtKB P93343.
SMR	P29310. Positions 4-235.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17371N.
IntAct	P29310.
Genome annotation databases
Ensembl	CG17870. Drosophila melanogaster. [Contig view]
GeneID	36059.
KEGG	dme:Dmel_CG17870.
Organism-specific databases
FlyBase	FBgn0004907. 14-3-3zeta.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-003789-MON. DMEL-XXX-02:DMEL-XXX-02-003790-MON.
Gene expression databases
ArrayExpress	P29310.
GermOnline	CG17870. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000308. 14-3-3. [Graphical view]
Gene3D	G3DSA:1.20.190.20. 14-3-3. 1 hit.
PANTHER	PTHR18860. 14-3-3. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
ProDom	PD000600. 14-3-3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

1433Z_DROME

Accession

Primary (citable) accession number: P29310
Secondary accession number(s): A4UZB2 , A4UZB3, O01665, Q0E9D5, Q0E9D6, Q0E9D7, Q8IGB9, Q8MKV5, Q9V5G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	July 22, 2008
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project