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Reviewed, UniProtKB/Swiss-Prot P29439 (DPO3B_BUCAP)

Last modified July 22, 2008. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA polymerase III subunit beta
    EC=2.7.7.7
Gene names
Name: dnaN
Ordered Locus Names: BUsg_011
OrganismBuchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]
Taxonomic identifier98794 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

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Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

Contains a core (composed of alpha, epsilon, and theta chains) that can repair short gaps created by nuclease in duplex DNA. For efficient replication of the long, single-stranded templates, pol III requires the auxiliary chains beta, gamma, and delta By similarity.

Subcellular location

CytoplasmBy similarity.

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Ontologies

Keywords

   Biological processDNA replication
   Cellular componentCytoplasm
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome

Gene Ontology (GO)

None. [Check GOA]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 366366DNA polymerase III subunit beta

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Sequences

Sequence LengthMass (Da)Tools
P29439-1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 9432FAB06CEFFDF1

FASTA36642,180
        10         20         30         40         50         60 
MKFTIQNDIL TKNLKKITRV LVKNISFPIL ENILIQVEDG TLSLTTTNLE IELISKIEII 

        70         80         90        100        110        120 
TKYIPGKTTI SGRKILNICR TLSEKSKIKM QLKNKKMYIS SENSNYILST LSADTFPNHQ 

       130        140        150        160        170        180 
NFDYISKFDI SSNILKEMIE KTEFSMGKQD VRYYLNGMLL EKKDKFLRSV ATDGYRLAIS 

       190        200        210        220        230        240 
YTQLKKDINF FSIIIPNKAV MELLKLLNTQ PQLLNILIGS NSIRIYTKNL IFTTQLIEGE 

       250        260        270        280        290        300 
YPDYKSVLFK EKKNPIITNS ILLKKSLLRV AILAHEKFCG IEIKIENGKF KVLSDNQEEE 

       310        320        330        340        350        360 
TAEDLFEIDY FGEKIEISIN VYYLLDVINN IKSENIALFL NKSKSSIQIE AENNSSNAYV 


VMLLKR

« Hide

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References

« Hide 'large scale' references
[1]"Genetic analysis of an aphid endosymbiont DNA fragment homologous to the rnpA-rpmH-dnaA-dnaN-gyrB region of eubacteria."
Lai C.-Y., Baumann P.
Gene 113:175-181(1992) [PubMed: 1572539] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho."
Clark M.A., Baumann L., Baumann P.
Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

M80817 Genomic DNA. Translation: AAA73150.1.
AF008210 Genomic DNA. Translation: AAC38107.1.
AE013218 Genomic DNA. Translation: AAM67583.1.
PIRJC1159.
RefSeqNP_660372.1.

3D structure databases

HSSPHSSP built from PDB template 2POL based on UniProtKB P00583.
ModBaseSearch...

Genome annotation databases

GeneID1005826.
GenomeReviewsGene locus BUsg_011 in contig AE013218_GR.
KEGGbas:BUsg011.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP29439.

Enzyme and pathway databases

BioCycBAPH198804:BUSG011-MON.

Family and domain databases

InterProIPR001001. DNA_polIII_beta.
[Graphical view]
Gene3DG3DSA:3.10.150.10. DNA_polIII_beta. 3 hits.
PfamPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
SMARTSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00663. dnan. 1 hit.
ProDomP29439.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameDPO3B_BUCAP
AccessionPrimary (citable) accession number: P29439
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents