Reviewed, UniProtKB/Swiss-Prot P30372 (ACM2_CHICK)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M2

Gene names

Name:	CHRM2
Synonyms:	CM2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Miscellaneous	This receptor has a high affinity for pirenzepine.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 466	466	Muscarinic acetylcholine receptor M2
Regions
	Topological domain	1 – 25	25	Extracellular Potential
	Transmembrane	26 – 48	23	1 Potential
	Topological domain	49 – 62	14	Cytoplasmic Potential
	Transmembrane	63 – 83	21	2 Potential
	Topological domain	84 – 100	17	Extracellular Potential
	Transmembrane	101 – 122	22	3 Potential
	Topological domain	123 – 142	20	Cytoplasmic Potential
	Transmembrane	143 – 165	23	4 Potential
	Topological domain	166 – 187	22	Extracellular Potential
	Transmembrane	188 – 210	23	5 Potential
	Topological domain	211 – 388	178	Cytoplasmic Potential
	Transmembrane	389 – 409	21	6 Potential
	Topological domain	410 – 423	14	Extracellular Potential
	Transmembrane	424 – 443	20	7 Potential
	Topological domain	444 – 466	23	Cytoplasmic Potential
Amino acid modifications
	Modified residue	446	1	Phosphothreonine Potential
	Modified residue	450	1	Phosphothreonine Potential
	Modified residue	465	1	Phosphothreonine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Disulfide bond	99 ↔ 179		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30372-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 2E3D8241D6168995	FASTA	466	51,565
	10 20 30 40 50 60 MNNSTYINSS SENVIALESP YKTIEVVFIV LVAGSLSLVT IIGNILVMVS IKVNRHLQTV 70 80 90 100 110 120 NNYFLFSLAC ADLIIGIFSM NLYTLYTVIG YWPLGPVVCD LWLALDYVVS NASVMNLLII 130 140 150 160 170 180 SFDRYFCVTK PLTYPVKRTT KMAGMMIAAA WVLSFILWAP AILFWQFIVG GRTVPDKDCY 190 200 210 220 230 240 IQFFSNPAVT FGTAIAAFYL PVIIMTVLYW QISRASKSRI KKGKKEAAQN QDPVSPSLVQ 250 260 270 280 290 300 GKIVKPNNNN IPTSSDGLEH NKVQNGKTTG ESVMENCVQG EEKDSSNDST SVSVVPSNTK 310 320 330 340 350 360 EDEAAKDASQ ISASQDHLKV ENSKLTCIRI VTKSQKGDCC APTNTTVEIV GTNGDEKQNS 370 380 390 400 410 420 VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW TPYNVMVLIN SFCASCIPGT 430 440 450 460 VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR

References

[1]

"Embryonic chick heart expresses multiple muscarinic acetylcholine receptor subtypes. Isolation and characterization of a gene encoding a novel m2 muscarinic acetylcholine receptor with high affinity for pirenzepine." Tietje K.M., Nathanson N.M. J. Biol. Chem. 266:17382-17387(1991) [PubMed: 1840593] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73217 Genomic DNA. Translation: AAB04106.1.
PIR	A40972.
RefSeq	NP_001025936.1.
UniGene	Gga.10240
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSGALG00000012894. Gallus gallus. [Contig view]
GeneID	418126.
KEGG	gga:418126.
Phylogenomic databases
HOGENOM	P30372.
HOVERGEN	P30372.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001065. Musac_M2_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF32. Musac_M2_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00539. MUSCRINICM2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P30372. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM2_CHICK

Accession

Primary (citable) accession number: P30372

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	July 22, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)