Reviewed, UniProtKB/Swiss-Prot P30411 (BKRB2_HUMAN)

Names and origin

Protein names

Recommended name:
    B2 bradykinin receptor
Alternative name(s):
    BK-2 receptor
      Short name=B2R

Gene names

Name:	BDKRB2
Synonyms:	BKR2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitous. Widespread in normal smooth muscle tissue and neurons.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Ref.1 Traceable author statement. Source: ProtInc arachidonic acid secretion Inferred from direct assay. Source: UniProtKB elevation of cytosolic calcium ion concentration Traceable author statement. Source: ProtInc inflammatory response Inferred by curator. Source: UniProtKB regulation of vascular permeability Inferred by curator. Source: UniProtKB regulation of vasoconstriction Inferred by curator. Source: UniProtKB smooth muscle contraction Inferred by curator. Source: UniProtKB transmembrane receptor protein tyrosine kinase signaling pathway Traceable author statement. Source: ProtInc
Cellular component	endosome Inferred from direct assay. Source: UniProtKB integral to plasma membrane Ref.2 Traceable author statement. Source: ProtInc
Molecular function	bradykinin receptor activity Traceable author statement. Source: ProtInc phosphoinositide phospholipase C activity Traceable author statement. Source: ProtInc protease binding Inferred from physical interaction. Source: UniProtKB protein heterodimerization activity Inferred from physical interaction. Source: UniProtKB type 1 angiotensin receptor binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P30411-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P30411-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 391	391	B2 bradykinin receptor
Regions
	Topological domain	1 – 60	60	Extracellular Potential
	Transmembrane	61 – 84	24	1 Potential
	Topological domain	85 – 93	9	Cytoplasmic Potential
	Transmembrane	94 – 118	25	2 Potential
	Topological domain	119 – 131	13	Extracellular Potential
	Transmembrane	132 – 153	22	3 Potential
	Topological domain	154 – 175	22	Cytoplasmic Potential
	Transmembrane	176 – 198	23	4 Potential
	Topological domain	199 – 221	23	Extracellular Potential
	Transmembrane	222 – 248	27	5 Potential
	Topological domain	249 – 267	19	Cytoplasmic Potential
	Transmembrane	268 – 292	25	6 Potential
	Topological domain	293 – 311	19	Extracellular Potential
	Transmembrane	312 – 335	24	7 Potential
	Topological domain	336 – 391	56	Cytoplasmic Potential
Amino acid modifications
	Lipidation	351	1	S-palmitoyl cysteine Potential
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Disulfide bond	130 ↔ 211		By similarity
Natural variations
	Alternative sequence	1 – 27	27	Missing in isoform Short.
	Natural variant	14	1	R → C: dbSNP rs1046248.
	Natural variant	354	1	G → E: dbSNP rs2227279.

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 066940ED3D18BB66	FASTA	391	44,461
	10 20 30 40 50 60 MFSPWKISMF LSVREDSVPT TASFSADMLN VTLQGPTLNG TFAQSKCPQV EWLGWLNTIQ 70 80 90 100 110 120 PPFLWVLFVL ATLENIFVLS VFCLHKSSCT VAEIYLGNLA AADLILACGL PFWAITISNN 130 140 150 160 170 180 FDWLFGETLC RVVNAIISMN LYSSICFLML VSIDRYLALV KTMSMGRMRG VRWAKLYSLV 190 200 210 220 230 240 IWGCTLLLSS PMLVFRTMKE YSDEGHNVTA CVISYPSLIW EVFTNMLLNV VGFLLPLSVI 250 260 270 280 290 300 TFCTMQIMQV LRNNEMQKFK EIQTERRATV LVLVVLLLFI ICWLPFQIST FLDTLHRLGI 310 320 330 340 350 360 LSSCQDERII DVITQIASFM AYSNSCLNPL VYVIVGKRFR KKSWEVYQGV CQKGGCRSEP 370 380 390 IQMENSMGTL RTSISVERQI HKLQDWAGSR Q
	Isoform Short [UniParc]. Checksum: 8FDC67EFC6600FB6		364	41,441
	10 20 30 40 50 60 MLNVTLQGPT LNGTFAQSKC PQVEWLGWLN TIQPPFLWVL FVLATLENIF VLSVFCLHKS 70 80 90 100 110 120 SCTVAEIYLG NLAAADLILA CGLPFWAITI SNNFDWLFGE TLCRVVNAII SMNLYSSICF 130 140 150 160 170 180 LMLVSIDRYL ALVKTMSMGR MRGVRWAKLY SLVIWGCTLL LSSPMLVFRT MKEYSDEGHN 190 200 210 220 230 240 VTACVISYPS LIWEVFTNML LNVVGFLLPL SVITFCTMQI MQVLRNNEMQ KFKEIQTERR 250 260 270 280 290 300 ATVLVLVVLL LFIICWLPFQ ISTFLDTLHR LGILSSCQDE RIIDVITQIA SFMAYSNSCL 310 320 330 340 350 360 NPLVYVIVGK RFRKKSWEVY QGVCQKGGCR SEPIQMENSM GTLRTSISVE RQIHKLQDWA GSRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and pharmacological characterization of a human bradykinin (BK-2) receptor." Hess J.F.R., Borkowski J.A., Young G.S., Strader C.D., Ransom R.W. Biochem. Biophys. Res. Commun. 184:260-268(1992) [PubMed: 1314587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]	"Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14." Powell S.J., Slynn G., Thomas C., Hopkins B., Briggs I., Graham A. Genomics 15:435-438(1993) [PubMed: 7916737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
[3]	"Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity." McIntyre P., Phillips E., Skidmore E., Brown M., Webb M. Mol. Pharmacol. 44:346-355(1993) [PubMed: 8394991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[4]	"Molecular cloning, functional expression and pharmacological characterization of a human bradykinin B2 receptor gene." Eggerickx D., Raspe E., Bertrand D., Vassart G., Parmentier M. Biochem. Biophys. Res. Commun. 187:1306-1313(1992) [PubMed: 1329734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
[5]	"Structure and chromosomal localization of the gene (BDKRB2) encoding human bradykinin B2 receptor." Ma J.-X., Wang D.-Z., Ward D.C., Chen L., Dessai T., Chao J., Chao L. Genomics 23:362-369(1994) [PubMed: 7835885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), TISSUE SPECIFICITY.
[6]	"Identification of polymorphic sites of the human bradykinin B2 receptor gene." Braun A., Kammerer S., Boehme E., Mueller B., Roscher A.A. Biochem. Biophys. Res. Commun. 211:234-240(1995) [PubMed: 7779090] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), VARIANT CYS-14.
[7]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Warren C.N., Aronstam R.S., Sharma S.V. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Lung.
[8]	SeattleSNPs program for genomic applications Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-14 AND GLU-354.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Lung.
[10]	"Structure of the bradykinin B2 receptors' amino terminus." Abdalla S., Godovac-Zimmermann J., Braun A., Roscher A.A., Mueller-Esterl W., Quitterer U. Biochemistry 35:7514-7519(1996) [PubMed: 8652530] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-9 AND 17-30. Tissue: Foreskin.
+	Additional computationally mapped references.

Web resources

Wikipedia

Bradykinin receptor entry

SeattleSNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88714 mRNA. Translation: AAB02793.1. S56772 Genomic DNA. Translation: AAB25765.1. X69680 mRNA. Translation: CAA49360.1. S45489 Genomic DNA. Translation: AAB23467.1. L27594 Genomic DNA. No translation available. X86165 mRNA. Translation: CAA60109.1. X86164 mRNA. Translation: CAA60108.1. AY275465 mRNA. Translation: AAP32297.1. AF378542 Genomic DNA. Translation: AAK56376.1. BC074894 mRNA. Translation: AAH74894.1. BC074895 mRNA. Translation: AAH74895.1.
PIR	JQ1488. JH0712.
RefSeq	NP_000614.1.
UniGene	Hs.654542
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30411.
Genome annotation databases
Ensembl	ENSG00000168398. Homo sapiens. [Contig view]
GeneID	624.
KEGG	hsa:624.
Organism-specific databases
H-InvDB	HIX0037700.
HGNC	HGNC:1030. BDKRB2.
MIM	113503. gene.
PharmGKB	PA80.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P30411.
HOVERGEN	P30411.
Gene expression databases
ArrayExpress	P30411.
CleanEx	HS_BDKRB2.
GermOnline	ENSG00000168398. Homo sapiens.
Family and domain databases
InterPro	IPR001504. B2_bradkn_rcpt. IPR000496. Brdyknn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00425. BRADYKININR. PR00994. BRADYKINNB2R. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P30411. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

BKRB2_HUMAN

Accession

Primary (citable) accession number: P30411

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)