Reviewed, UniProtKB/Swiss-Prot P30543 (AA2AR_RAT)

Names and origin

Protein names

Recommended name:
    Adenosine receptor A2a

Gene names

Name:

Adora2a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from Experiment. Source: Reactome
Molecular function	adenosine receptor activity, G-protein coupled Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 410	410	Adenosine receptor A2a
Regions
	Topological domain	1 – 4	4	Extracellular Potential
	Transmembrane	5 – 27	23	1 Potential
	Topological domain	28 – 40	13	Cytoplasmic Potential
	Transmembrane	41 – 63	23	2 Potential
	Topological domain	64 – 74	11	Extracellular Potential
	Transmembrane	75 – 97	23	3 Potential
	Topological domain	98 – 117	20	Cytoplasmic Potential
	Transmembrane	118 – 140	23	4 Potential
	Topological domain	141 – 168	28	Extracellular Potential
	Transmembrane	169 – 193	25	5 Potential
	Topological domain	194 – 229	36	Cytoplasmic Potential
	Transmembrane	230 – 253	24	6 Potential
	Topological domain	254 – 261	8	Extracellular Potential
	Transmembrane	262 – 285	24	7 Potential
	Topological domain	286 – 410	125	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Disulfide bond	74 ↔ 161		By similarity
Experimental info
	Sequence conflict	183	1	L → T in AAA70305. Ref.1
	Sequence conflict	268	1	A → T in AAA70305. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30543-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: D87B256CD2336BAC	FASTA	410	44,999
	10 20 30 40 50 60 MGSSVYITVE LAIAVLAILG NVLVCWAVWI NSNLQNVTNF FVVSLAAADI AVGVLAIPFA 70 80 90 100 110 120 ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLAIAIDRY IAIRIPLRYN GLVTGVRAKG 130 140 150 160 170 180 IIAICWVLSF AIGLTPMLGW NNCSQKDGNS TKTCGEGRVT CLFEDVVPMN YMVYYNFFAF 190 200 210 220 230 240 VLLPLLLMLA IYLRIFLAAR RQLKQMESQP LPGERTRSTL QKEVHAAKSL AIIVGLFALC 250 260 270 280 290 300 WLPLHIINCF TFFCSTCRHA PPWLMYLAII LSHSNSVVNP FIYAYRIREF RQTFRKIIRT 310 320 330 340 350 360 HVLRRQEPFQ AGGSSAWALA AHSTEGEQVS LRLNGHPLGV WANGSATHSG RRPNGYTLGL 370 380 390 400 410 GGGGSAQGSP RDVELPTQER QEGQEHPGLR GHLVQARVGA SSWSSEFAPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel adenosine receptor gene from rat brain." Chern Y., King K., Lai H.-L., Lai H.-T. Biochem. Biophys. Res. Commun. 185:304-309(1992) [PubMed: 1599465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Molecular cloning of the rat A2 adenosine receptor: selective co-expression with D2 dopamine receptors in rat striatum." Fink J.S., Weaver D.R., Rivkees S.A., Peterfreund R.A., Pollack A.E., Adler E.M., Reppert S.M. Brain Res. Mol. Brain Res. 14:186-195(1992) [PubMed: 1279342] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Corpus striatum.
[3]	"Rattus norvegicus mucosa A2A adenosine receptor mRNA." Yip L., Hewitt J., Kwok Y. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M91214 mRNA. Translation: AAA70305.1. L08102 mRNA. No translation available. S47609 mRNA. Translation: AAA11888.1. AF228684 mRNA. Translation: AAF61924.1. BC081727 mRNA. Translation: AAH81727.1.
PIR	A48974.
RefSeq	NP_445746.3.
UniGene	Rn.11180
3D structure databases
HSSP	HSSP built from PDB template 1MMH based on UniProtKB P29274.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSRNOG00000001302. Rattus norvegicus. [Contig view]
GeneID	25369.
KEGG	rno:25369.
Organism-specific databases
RGD	2049. Adora2a.
Phylogenomic databases
HOVERGEN	P30543.
Gene expression databases
ArrayExpress	P30543.
GermOnline	ENSRNOG00000001302. Rattus norvegicus.
Family and domain databases
InterPro	IPR001513. Adeno_A2A_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00553. ADENOSINA2AR. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P30543. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AA2AR_RAT

Accession

Primary (citable) accession number: P30543

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)