Reviewed, UniProtKB/Swiss-Prot P31115 (PUS3_YEAST)

Names and origin

Protein names

Recommended name:
    tRNA pseudouridine synthase 3
    EC=5.4.99.-
Alternative name(s):
    tRNA-uridine isomerase 3
    tRNA pseudouridylate synthase 3
    Depressed growth-rate protein DEG1

Gene names

Name:	PUS3
Synonyms:	DEG1
Ordered Locus Names:	YFL001W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Formation of pseudouridines at positions 38 and 39 in the anticodon stem and loop of transfer RNAs.
Catalytic activity	tRNA uridine = tRNA pseudouridine.
Subcellular location	Nucleus.
Miscellaneous	Present with 5860 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the tRNA pseudouridine synthase truA family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA pseudouridine synthesis Ref.5 Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Ref.5 Inferred from direct assay. Source: SGD nucleus Ref.5 Inferred from direct assay. Source: SGD
Molecular function	pseudouridine synthase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 442	442	tRNA pseudouridine synthase 3
Sites
	Active site	151	1	Nucleophile By similarity
Amino acid modifications
	Modified residue	68	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P31115-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 89C35917366BDB9B	FASTA	442	50,889
	10 20 30 40 50 60 MSNFIRRLVG KMKAISTGTN AIVSKKDSIY ANWSKEQLIR RITELENANK PHSEKFQHIE 70 80 90 100 110 120 DNKKRKISQE EVTRSKAKKA PKKFDFSKHN TRFIALRFAY LGWNYNGLAV QKEYTPLPTV 130 140 150 160 170 180 EGTILEAMNK CKLVPSMVLQ DYKFSRCGRT DKGVSAMNQV ISLEVRSNLT DEEQRDPTND 190 200 210 220 230 240 SREIPYVHVL NQLLPDDIRI SAVCLRPPPN FDARFSCVHR HYKYIFNGKN LNIEKMSKAA 250 260 270 280 290 300 SYFVGERDFR NFCKLDGSKQ ITNFKRTIIS SKILPLSETF YCFDLVGSAF LWHQVRCMMA 310 320 330 340 350 360 ILFLVGQSLE VPEIVLRLTD IEKTPQRPVY EMANDIPLLL YDCKFPEMDW QEPTVDDYKA 370 380 390 400 410 420 IKFTTATEAL TLHYELKAAV CNIFKDVLPT ANTNNFSKTI INLGDGRGKV VGTYVKLEDR 430 440 SVMEPVEVVN AKYSKKKNNK NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae." Agostoni Carbone M.L., Solinas M., Sora S., Panzeri L. Curr. Genet. 19:1-8(1991) [PubMed: 2036682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI." Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T., Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T., Murakami Y. Yeast 12:77-84(1996) [PubMed: 8789262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop." Lecointe F., Simos G., Sauer A., Hurt E.C., Motorin Y., Grosjean H. J. Biol. Chem. 273:1316-1323(1998) [PubMed: 9430663] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09239.1. AY693197 Genomic DNA. Translation: AAT93216.1.
PIR	S14145.
RefSeq	NP_116655.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5151N.
IntAct	P31115.
Proteomic databases
PeptideAtlas	P31115.
Genome annotation databases
Ensembl	YFL001W. Saccharomyces cerevisiae. [Contig view]
GeneID	850550.
GenomeReviews	Gene locus YFL001W in contig D50617_GR.
KEGG	sce:YFL001W.
NMPDR	fig|4932.3.peg.2286.
Organism-specific databases
CYGD	YFL001w.
SGD	S000001895. DEG1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P31115.
Gene expression databases
ArrayExpress	P31115.
GermOnline	YFL001W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001406. tRNA_psdUrid_synth. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProDom	P31115. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P31115.
ProtoNet	Search...

Entry information

Entry name

PUS3_YEAST

Accession

Primary (citable) accession number: P31115

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 2, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)