Reviewed, UniProtKB/Swiss-Prot P31117 (T2C2_PBCV1)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme CviAII
      Short name=R.CviAII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme CviAII
    Endonuclease CviAII

Gene names

Name:	CVIAIIR
Ordered Locus Names:	A252R

Organism

Paramecium bursaria Chlorella virus 1 (PBCV-1)

Taxonomic identifier

10506 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus

Virus host

Chlorella [TaxID: 114049]

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CATG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 342	342	Type-2 restriction enzyme CviAII

Sequences

	Sequence		Length	Mass (Da)
	P31117-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: D553D28BD6300522	FASTA	342	39,928
	10 20 30 40 50 60 MTQKILNPVT GRFVKVDGST GKKIKTGNIY DVNNILSSKL TKKIFDKIRR NDLAKEERET 70 80 90 100 110 120 QQLTKKISKG IFDKIRSENK KYEKNHQKLT KKMVLDIFSK IYKEDSRKSK TQCVVSEKKD 130 140 150 160 170 180 NGGVLLTEDL GKIFEKSICM LYDTPYIGPY KYGNEKPMLL KTRLHKLLDF FPELTHTAAG 190 200 210 220 230 240 GALHDFTTKN SRYLSAKTSK KKDGKVAPQK IGQPTKKKFL EFFNLPPDTS NDDIKLFIKK 250 260 270 280 290 300 NIVRILDEYF KYTFDDTIIY YNEVNNIIML VKTLKKVKFD PNLIEFGCNK PGKSWKESTI 310 320 330 340 LFYNNKRLGE FQIHTSRSCI KFRWFFENIL LLFPDNFEVT IL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system." Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L. Nucleic Acids Res. 20:5351-5356(1992) [PubMed: 1437552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of 94 kb of the chlorella virus PBCV-1 330-kb genome: map positions 88 to 182." Lu Z., Li Y., Que Q., Kutish G.F., Rock D.L., van Etten J.L. Virology 216:102-123(1996) [PubMed: 8614977] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86639 Genomic DNA. Translation: AAB92382.1. U42580 Genomic DNA. Translation: AAC96620.1.
PIR	S27902. S35443.
RefSeq	NP_048602.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2211. CviAII.
Genome annotation databases
GeneID	918300.
Family and domain databases
ProDom	P31117. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

T2C2_PBCV1

Accession

Primary (citable) accession number: P31117

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 2, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)