Reviewed, UniProtKB/Swiss-Prot P31206 (NANH_BACFR)

Names and origin

Protein names

Recommended name:
    Sialidase
    EC=3.2.1.18
Alternative name(s):
    Neuraminidase

Gene names

Name:	nanH
Ordered Locus Names:	BF1729

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Sialidases have been suggested to be pathogenic factors in microbial infections.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 5 BNR repeats.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 544	522	Sialidase		PRO_0000012029
Regions
	Repeat	239 – 250	12	BNR 1
	Repeat	318 – 329	12	BNR 2
	Repeat	378 – 389	12	BNR 3
	Repeat	425 – 436	12	BNR 4
	Repeat	485 – 496	12	BNR 5
Sites
	Active site	399	1	Potential
	Binding site	415	1	Substrate By similarity
	Binding site	479	1	Substrate By similarity
Experimental info
	Sequence conflict	197	1	D → A in BAA05853. Ref.1
	Sequence conflict	405	1	V → A in AAA22912. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P31206-1 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 6D1D185AB15FA822	FASTA	544	59,556
	10 20 30 40 50 60 MKKAVILFSL FCFLCAIPVV QAADTIFVRE TRIPILIERQ DNVLFYLRLD AKESQTLNDV 70 80 90 100 110 120 VLNLGEGVNL SEIQSIKLYY GGTEALQDSG KKRFAPVGYI SSNTPGKTLA ANPSYSIKKS 130 140 150 160 170 180 EVTNPGNQVV LKGDQKLFPG INYFWISLQM KPGTSLTSKV TADIASITLD GKKALLDVVS 190 200 210 220 230 240 ENGIEHRMGV GVRHAGDDNS AAFRIPGLVT TNKGTLLGVY DVRYNSSVDL QEHVDVGLSR 250 260 270 280 290 300 STDGGKTWEK MRLPLAFGEF GGLPAGQNGV GDPSILVDTK TNNVWVVAAW THGMGNQRAW 310 320 330 340 350 360 WSSHPGMDMN HTAQLVLAKS TDDGKTWSAP INITEQVKDP SWYFLLQGPG RGITMSDGTL 370 380 390 400 410 420 VFPTQFIDST RVPNAGIMYS KDGGKNWKMH NYARTNTTEA QVAEVEPGVL MLNMRDNRGG 430 440 450 460 470 480 SRAVAITKDL GKTWTEHESS RKALPESVCM ASLISVKAKD NVLGKDLLIF SNPNTTKGRY 490 500 510 520 530 540 NTTIKISLDG GVTWSPEHQL LLDEGNNWGY SCLSMIDKET IGILYESSVA HMTFQAVKLK DIIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of the Bacteroides fragilis YCH46 neuraminidase-encoding gene." Akimoto S., Ono T., Tsutsui H., Kinouchi T., Kataoka K., Ohnishi Y. Biochem. Biophys. Res. Commun. 203:914-921(1994) [PubMed: 8093075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YCH46.
[2]	"Characterization of a gene cluster for degradation of sialoglycoconjugates in Bacteroides fragilis strain YCH46." Nakayama H., Kuwahara T., Iwasa T., Okamoto S., Tsuchihashi Y., Nakanishi K., Kataoka K., Arimochi H., Ohnishi Y. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YCH46.
[3]	"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.
[4]	"Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli." Russo T.A., Thompson J.S., Godoy V.G., Malamy M.H. J. Bacteriol. 172:2594-2600(1990) [PubMed: 2158974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 235-500. Strain: TAL2480.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D28493 Genomic DNA. Translation: BAA05853.1. AB102772 Genomic DNA. Translation: BAC56895.2. AP006841 Genomic DNA. Translation: BAD48476.1. M31663 Genomic DNA. Translation: AAA22912.1.
PIR	JC2500.
RefSeq	YP_099010.1.
3D structure databases
HSSP	HSSP built from PDB template 1EUR based on UniProtKB Q02834.
ModBase	Search...
Genome annotation databases
GeneID	3082956.
GenomeReviews	Gene locus BF1729 in contig AP006841_GR.
KEGG	bfr:BF1729.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P31206.
Enzyme and pathway databases
BioCyc	BFRA295405:BF1729-MON.
Family and domain databases
InterPro	IPR002860. BNR. IPR008377. Sialidase_trypan. [Graphical view]
Pfam	PF02012. BNR. 5 hits. [Graphical view]
PRINTS	PR01803. TCSIALIDASE.
ProtoNet	Search...

Entry information

Entry name

NANH_BACFR

Accession

Primary (citable) accession number: P31206
Secondary accession number(s): Q45145, Q64VK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 23, 2004
Last modified:	November 25, 2008
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)