Reviewed, UniProtKB/Swiss-Prot P31310 (HXA10_MOUSE)

Names and origin

Protein names

Recommended name:
    Homeobox protein Hox-A10
Alternative name(s):
    Hox-1.8

Gene names

Name:	Hoxa10
Synonyms:	Hox-1.8, Hoxa-10

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to the DNA sequence 5'-AA[AT]TTTTATTAC-3'.
Subcellular location	Nucleus.
Tissue specificity	Expressed in the developing limb bud where it is restricted to the mesenchyme along the proximal-distal axis. Also found in developing gut and urogenital tract. In adult tissue, both forms found in kidney but only isoform 1 is expressed in skeletal muscle.
Developmental stage	Embryonic expression increases from day 9 to day 12 and then declines to day 15.
Sequence similarities	Belongs to the Abd-B homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Gene Ontology (GO)
Biological process	anterior/posterior pattern formation Inferred from genetic interaction. Source: MGI embryonic limb morphogenesis Inferred from genetic interaction. Source: MGI proximal/distal pattern formation Inferred from genetic interaction. Source: MGI skeletal development Inferred from genetic interaction. Source: MGI
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	DNA binding Traceable author statement. Source: MGI protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gmnn	O88513	1	EBI-446033,EBI-445922

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P31310-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P31310-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-305: Missing.
     306-308: DSL → MCQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 399	399	Homeobox protein Hox-A10
Regions
	DNA binding	325 – 384	60	Homeobox
	Compositional bias	26 – 38	13	Gly-rich
	Compositional bias	77 – 90	14	Poly-Gly
	Compositional bias	113 – 144	32	Gln/Pro-rich
	Compositional bias	215 – 222	8	Poly-Gly
	Compositional bias	271 – 274	4	Poly-Gly
Natural variations
	Alternative sequence	1 – 305	305	Missing in isoform 2.
	Alternative sequence	306 – 308	3	DSL → MCQ in isoform 2.
Experimental info
	Sequence conflict	27	1	V → G in AAH50839. Ref.2
	Sequence conflict	224	1	Q → E in AAA67125. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 5, 2006. Version 3. Checksum: 03FD7640B0DE0692	FASTA	399	41,414
	10 20 30 40 50 60 MSCSESPAAN SFLVDSLISS GRGEAGVGGG SAGGGGGGYY AHGGVYLPPA SDLPYGLQSC 70 80 90 100 110 120 GLFPALGSKR NEAPSPGGGG GGGSGGLGPG THGYAPAPLD LWLDAPRSCR MEPPDGPPPP 130 140 150 160 170 180 QPQPQQQQQQ PPPPPPQPPQ PQPQATSCSF AQNIKEESSY CLYDAADKCP KGSAAADLAP 190 200 210 220 230 240 FPRGPPPDGC ALGASSGVPV PGYFRLSQAY GTAKGFGSGG GGTQQLASPF PAQPPGRGFD 250 260 270 280 290 300 PPPALASGST EAAGKERVLD STPPPTLVCT GGGGSQGDEE AHASSSAAEE LSPAPSENSK 310 320 330 340 350 360 ASPEKDSLGS SKGENAANWL TAKSGRKKRC PYTKHQTLEL EKEFLFNMYL TRERRLEISR 370 380 390 SVHLTDRQVK IWFQNRRMKL KKMNRENRIR ELTANFNFS
	Isoform 2 [UniParc]. Checksum: 1F40E359CAFD821F		94	11,425
	10 20 30 40 50 60 MCQGSSKGEN AANWLTAKSG RKKRCPYTKH QTLELEKEFL FNMYLTRERR LEISRSVHLT 70 80 90 DRQVKIWFQN RRMKLKKMNR ENRIRELTAN FNFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The expression pattern of the murine Hoxa-10 gene and the sequence recognition of its homeodomain reveal specific properties of Abdominal B-like genes." Benson G.V., Nguyen T.-H.E., Maas R.L. Mol. Cell. Biol. 15:1591-1601(1995) [PubMed: 7862151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Limb.
[3]	"Identification of 10 murine homeobox genes." Singh G., Kaur S., Stock J.L., Jenkins N.A., Gilbert D.J., Copeland N.G., Potter S.S. Proc. Natl. Acad. Sci. U.S.A. 88:10706-10710(1991) [PubMed: 1683707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 325-384.
[4]	"Detection of homeobox genes in development and evolution." Murtha M.T., Leckman J.F., Ruddle F.H. Proc. Natl. Acad. Sci. U.S.A. 88:10711-10715(1991) [PubMed: 1720547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 346-370. Strain: C57BL/6. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L08757 mRNA. Translation: AAA67125.1. BC050839 mRNA. Translation: AAH50839.1. M81659 Genomic DNA. Translation: AAA63312.1.
PIR	I49754.
UniGene	Mm.5
3D structure databases
HSSP	HSSP built from PDB template 9ANT based on UniProtKB P02833.
SMR	P31310. Positions 316-386.
ModBase	Search...
Protein-protein interaction databases
IntAct	P31310.
PTM databases
PhosphoSite	P31310.
Genome annotation databases
Ensembl	ENSMUSG00000000938. Mus musculus. [Contig view]
NMPDR	fig|10090.3.peg.14401.
Organism-specific databases
MGI	MGI:96171. Hoxa10.
Phylogenomic databases
HOGENOM	P31310.
HOVERGEN	P31310.
Gene expression databases
ArrayExpress	P31310.
CleanEx	MM_HOXA10.
GermOnline	ENSMUSG00000000938. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
ProDom	PD000010. Homeobox. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

HXA10_MOUSE

Accession

Primary (citable) accession number: P31310
Secondary accession number(s): Q80Y22

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	September 5, 2006
Last modified:	September 2, 2008
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)