Reviewed, UniProtKB/Swiss-Prot P31974 (MTA1_CELCE)

Names and origin

Protein names

Recommended name:
    Modification methylase AluI
      Short name=M.AluI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase AluI

Gene names

Name:

aluIM

Organism

Cellulosimicrobium cellulans (Arthrobacter luteus)

Taxonomic identifier

1710 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Promicromonosporaceae › Cellulosimicrobium

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence AGCT, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the AluI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 521	521	Modification methylase AluI
Sites
	Active site	84	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31974-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: ACBBB2C4E5326BFB	FASTA	521	58,648
	10 20 30 40 50 60 MSKANAKYSF VDLFAGIGGF HAALAATGGV CEYAVEIDRE AAAVYERNWN KPALGDITDD 70 80 90 100 110 120 ANDEGVTLRG YDGPIDVLTG GFPCQPFSKS GAQHGMAETR GTLFWNIARI IEEREPTVLI 130 140 150 160 170 180 LENVRNLVGP RHRHEWLTII ETLRFFGYEV SGAPAIFSPH LLPAWMGGTP QVRERVFITA 190 200 210 220 230 240 TLVPERMRDE RIPRTETGEI DAEAIGPKPV ATMNDRFPIK KGGTELFHPG DRKSGWNLLT 250 260 270 280 290 300 SGIIREGDPE PSNVDLRLTE TETLWIDAWD DLESTIRRAT GRPLEGFPYW ADSWTDFREL 310 320 330 340 350 360 SRLVVIRGFQ APEREVVGDR KRYVARTDMP EGFVPASVTR PAIDETLPAW KQSHLRRNYD 370 380 390 400 410 420 FFERHFAEVV AWAYRWGVYT DLFPASRRKL EWQAQDAPRL WDTVMHFRPS GIRAKRPTYL 430 440 450 460 470 480 PALVAITQTS IVGPLERRLS PRETARLQGL PEWFDFGEQR AAATYKQMGN GVNVGVVRHI 490 500 510 520 LREHVRRDRA LLKLTPAGQR IINAVLADEP DATVGALGAA E

References

[1]

"The M.AluI DNA-(cytosine C5)-methyltransferase has an unusually large, partially dispensable, variable region." Zhang B., Tao T., Wilson G.G., Blumenthal R.M. Nucleic Acids Res. 21:905-911(1993) [PubMed: 8451189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 21606 / DSM 20424 / 73-14.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11841 Genomic DNA. Translation: CAA77866.1.
PIR	S35614.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProDom	P31974. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTA1_CELCE

Accession

Primary (citable) accession number: P31974

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 2, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)