Reviewed, UniProtKB/Swiss-Prot P31994 (FCG2B_HUMAN)

Names and origin

Protein names

Recommended name:
    Low affinity immunoglobulin gamma Fc region receptor II-b
      Short name=IgG Fc receptor II-b
Alternative name(s):
    Fc-gamma RII-b
    Fc-gamma-RIIb
      Short name=FcRII-b
    CDw32
    CD_antigen=CD32

Gene names

Name:	FCGR2B
Synonyms:	CD32, FCG2, IGFR2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Subunit structure	Isoform IIB1 interacts with measles virus N protein. N protein is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. Interacts with INPP5D/SHIP1.
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
Domain	Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Involvement in disease	A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
Sequence similarities	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Caution	Has sometimes been attributed to correspond to FcR-IIC.

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Chromosomal rearrangement Polymorphism
Disease	Proto-oncogene
Domain	Immunoglobulin domain Repeat Signal Transmembrane
Ligand	IgG-binding protein
Molecular function	Receptor
PTM	Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	immune response Ref.4 Traceable author statement. Source: ProtInc signal transduction Ref.4 Traceable author statement. Source: ProtInc
Cellular component	plasma membrane Non-traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IGHG1	P01857	2	EBI-724784,EBI-356114

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform IIB1 (identifier: P31994-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform IIB2 (identifier: P31994-2)

The sequence of this isoform differs from the canonical sequence as follows:
     254-272: Missing.

Isoform IIB3 (identifier: P31994-3)

The sequence of this isoform differs from the canonical sequence as follows:
     39-45: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 42

42

Potential

Chain

43 – 310

268

Low affinity immunoglobulin gamma Fc region receptor II-b

Regions

Topological domain

43 – 217

175

Extracellular Potential

Transmembrane

218 – 240

23

Potential

Topological domain

241 – 310

70

Cytoplasmic Potential

Domain

48 – 127

80

Ig-like C2-type 1

Domain

131 – 213

83

Ig-like C2-type 2

Motif

290 – 295

6

ITIM motif

Amino acid modifications

Glycosylation

106

1

N-linked (GlcNAc...) Potential

Glycosylation

180

1

N-linked (GlcNAc...) Potential

Glycosylation

187

1

N-linked (GlcNAc...) Potential

Disulfide bond

71 ↔ 113

Disulfide bond

152 ↔ 196

Natural variations

Alternative sequence

39 – 45

7

Missing in isoform IIB3.

Alternative sequence

254 – 272

19

Missing in isoform IIB2.

Natural variant

205

1

Y → F: dbSNP rs17416919.

Natural variant

232

1

I → T

Natural variant

258

1

Y → D

Experimental info

Sequence conflict

46

1

Missing in AAD00637, AAD00638, AAD00639 and AAD00644. Ref.1

Sequence conflict

178

1

D → I in CAA35644 and CAA35645. Ref.2

Sequence conflict

230

1

T → I in CAA35644 and CAA35645. Ref.2

Sequence conflict

242

1

V → G in CAA35644 and CAA35645. Ref.2

Sequence conflict

275

1

P → S Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

310

Helix Strand Turn

Details...

Beta strand

51 – 56

6

Beta strand

59 – 62

4

Beta strand

66 – 72

7

Beta strand

83 – 86

4

Beta strand

96 – 102

7

Helix

105 – 107

3

Beta strand

109 – 114

6

Beta strand

124 – 129

6

Beta strand

131 – 136

6

Beta strand

140 – 142

3

Beta strand

148 – 154

7

Helix

155 – 157

3

Beta strand

161 – 167

7

Beta strand

170 – 177

8

Beta strand

180 – 183

4

Helix

188 – 190

3

Beta strand

192 – 200

9

Beta strand

203 – 206

4

Beta strand

210 – 214

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform IIB1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 2186F8538FF01F36	FASTA	310	34,044
	10 20 30 40 50 60 MGILSFLPVL ATESDWADCK SPQPWGHMLL WTAVLFLAPV AGTPAAPPKA VLKLEPQWIN 70 80 90 100 110 120 VLQEDSVTLT CRGTHSPESD SIQWFHNGNL IPTHTQPSYR FKANNNDSGE YTCQTGQTSL 130 140 150 160 170 180 SDPVHLTVLS EWLVLQTPHL EFQEGETIVL RCHSWKDKPL VKVTFFQNGK SKKFSRSDPN 190 200 210 220 230 240 FSIPQANHSH SGDYHCTGNI GYTLYSSKPV TITVQAPSSS PMGIIVAVVT GIAVAAIVAA 250 260 270 280 290 300 VVALIYCRKK RISALPGYPE CREMGETLPE KPANPTNPDE ADKVGAENTI TYSLLMHPDA 310 LEEPDDQNRI
	Isoform IIB2 [UniParc]. Checksum: 3B76609541B30962		291	31,944
	10 20 30 40 50 60 MGILSFLPVL ATESDWADCK SPQPWGHMLL WTAVLFLAPV AGTPAAPPKA VLKLEPQWIN 70 80 90 100 110 120 VLQEDSVTLT CRGTHSPESD SIQWFHNGNL IPTHTQPSYR FKANNNDSGE YTCQTGQTSL 130 140 150 160 170 180 SDPVHLTVLS EWLVLQTPHL EFQEGETIVL RCHSWKDKPL VKVTFFQNGK SKKFSRSDPN 190 200 210 220 230 240 FSIPQANHSH SGDYHCTGNI GYTLYSSKPV TITVQAPSSS PMGIIVAVVT GIAVAAIVAA 250 260 270 280 290 VVALIYCRKK RISANPTNPD EADKVGAENT ITYSLLMHPD ALEEPDDQNR I
	Isoform IIB3 [UniParc]. Checksum: B84833348A175687		303	33,450
	10 20 30 40 50 60 MGILSFLPVL ATESDWADCK SPQPWGHMLL WTAVLFLAAP PKAVLKLEPQ WINVLQEDSV 70 80 90 100 110 120 TLTCRGTHSP ESDSIQWFHN GNLIPTHTQP SYRFKANNND SGEYTCQTGQ TSLSDPVHLT 130 140 150 160 170 180 VLSEWLVLQT PHLEFQEGET IVLRCHSWKD KPLVKVTFFQ NGKSKKFSRS DPNFSIPQAN 190 200 210 220 230 240 HSHSGDYHCT GNIGYTLYSS KPVTITVQAP SSSPMGIIVA VVTGIAVAAI VAAVVALIYC 250 260 270 280 290 300 RKKRISALPG YPECREMGET LPEKPANPTN PDEADKVGAE NTITYSLLMH PDALEEPDDQ NRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fc-gamma-RIIb nucleotide sequences in SLE and non-SLE humans in vivo derived lymphocytes." Ng S., Sinclair N.R.S., Anderson C., Bell D.A., Cairns E. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IIB1 AND IIB2). Tissue: Lymphocyte.
[2]	"Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium." Stuart S.G., Simister N.E., Clarkson S.B., Kacinski B.M., Shapiro M., Mellman I. EMBO J. 8:3657-3666(1989) [PubMed: 2531080] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB2). Tissue: Placenta.
[3]	"Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes." Brooks D.G., Qiu W.Q., Luster A.D., Ravetch J.V. J. Exp. Med. 170:1369-1385(1989) [PubMed: 2529342] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IIB1; IIB2 AND IIB3), VARIANT PHE-205.
[4]	"Distribution, inducibility and biological function of the cloned and expressed human beta Fc receptor II." Engelhardt W., Geerds C., Frey J. Eur. J. Immunol. 20:1367-1377(1990) [PubMed: 2142460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB2). Tissue: Placenta.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IIB2).
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IIB1). Tissue: Skin.
[8]	"Fc gamma receptor gene polymorphisms in Japanese patients with systemic lupus erythematosus: contribution of FCGR2B to genetic susceptibility." Kyogoku C., Dijstelbloem H.M., Tsuchiya N., Hatta Y., Kato H., Yamaguchi A., Fukazawa T., Jansen M.D., Hashimoto H., van de Winkel J.G.J., Kallenberg C.G.M., Tokunaga K. Arthritis Rheum. 46:1242-1254(2002) [PubMed: 12115230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 132-253, VARIANT THR-232.
[9]	"Measles virus nucleoprotein induces cell-proliferation arrest and apoptosis through NTAIL-NR and NCORE-FcgammaRIIB1 interactions, respectively." Laine D., Bourhis J.-M., Longhi S., Flacher M., Cassard L., Canard B., Sautes-Fridman C., Rabourdin-Combe C., Valentin H. J. Gen. Virol. 86:1771-1784(2005) [PubMed: 15914856] [Abstract] Cited for: INTERACTION WITH MEASLES VIRUS N PROTEIN.
[10]	"Crystal structure of the soluble form of the human fcgamma-receptor IIb: a new member of the immunoglobulin superfamily at 1.7 A resolution." Sondermann P., Huber R., Jacob U. EMBO J. 18:1095-1103(1999) [PubMed: 10064577] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF 46-217, DISULFIDE BONDS.
[11]	"Interaction of a human Fc gamma RIIb1 (CD32) isoform with murine and human IgG subclasses." Warmerdam P.A., van den Herik-Oudijk I.E., Parren P.W., Westerdaal N.A., van de Winkel J.G., Capel P.J. Int. Immunol. 5:239-247(1993) [PubMed: 8466861] [Abstract] Cited for: VARIANT ASP-258.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

U87560 mRNA. Translation: AAD00627.1. U87561 mRNA. Translation: AAD00628.1. U87562 mRNA. Translation: AAD00629.1. U87563 mRNA. Translation: AAD00630.1. U87564 mRNA. Translation: AAD00631.1. U87565 mRNA. Translation: AAD00632.1. U87566 mRNA. Translation: AAD00633.1. U87567 mRNA. Translation: AAD00634.1. U87568 mRNA. Translation: AAD00635.1. U87569 mRNA. Translation: AAD00636.1. U87570 mRNA. Translation: AAD00637.1. U87571 mRNA. Translation: AAD00638.1. U87572 mRNA. Translation: AAD00639.1. U87573 mRNA. Translation: AAD00640.1. U87574 mRNA. Translation: AAD00641.1. U87575 mRNA. Translation: AAD00642.1. U87576 mRNA. Translation: AAD00643.1.