Reviewed, UniProtKB/Swiss-Prot P32234 (128UP_DROME)

Names and origin

Protein names

Recommended name:
    GTP-binding protein 128up

Gene names

Name:	128up
Synonyms:	GTP-bp
ORF Names:	CG8340

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Deformed (Dfd) is required to activate 1.28up in maxillary segment cells.
Tissue specificity	Expressed in posterior-lateral epidermis of the maxillary lobe.
Developmental stage	Expressed in embryos and adults.
Sequence similarities	Belongs to the GTP1/OBG family.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Ref.1 Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VGZ4	1	EBI-163407,EBI-111903
nos	P25724	1	EBI-163407,EBI-106556
Sr-CI	Q9V3H7	1	EBI-163407,EBI-125222

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 368	368	GTP-binding protein 128up
Regions
	Nucleotide binding	71 – 78	8	GTP By similarity
	Nucleotide binding	117 – 121	5	GTP By similarity
	Nucleotide binding	248 – 251	4	GTP By similarity
Experimental info
	Sequence conflict	2	1	S → I in CAA50701. Ref.1
	Sequence conflict	34 – 35	2	KL → NV in CAA50701. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32234-1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 5B38B09D0C0A92F2	FASTA	368	41,132
	10 20 30 40 50 60 MSTILEKISA IESEMARTQK NKATSAHLGL LKAKLAKLRR ELISPKGGGG GTGEAGFEVA 70 80 90 100 110 120 KTGDARVGFV GFPSVGKSTL LSNLAGVYSE VAAYEFTTLT TVPGCIKYKG AKIQLLDLPG 130 140 150 160 170 180 IIEGAKDGKG RGRQVIAVAR TCNLIFMVLD CLKPLGHKKL LEHELEGFGI RLNKKPPNIY 190 200 210 220 230 240 YKRKDKGGIN LNSMVPQSEL DTDLVKTILS EYKIHNADIT LRYDATSDDL IDVIEGNRIY 250 260 270 280 290 300 IPCIYLLNKI DQISIEELDV IYKIPHCVPI SAHHHWNFDD LLELMWEYLR LQRIYTKPKG 310 320 330 340 350 360 QLPDYNSPVV LHNERTSIED FCNKLHRSIA KEFKYALVWG SSVKHQPQKV GIEHVLNDED VVQIVKKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene upstream of DmRP128 codes for a novel GTP-binding protein of Drosophila melanogaster." Sommer K.A., Petersen G., Bautz E.K.F. Mol. Gen. Genet. 242:391-398(1994) [PubMed: 8121394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Identification and characterization of a gene activated by the deformed homeoprotein." Mahaffey J.W., Jones D.F., Hickel J.A., Griswold C.M. Development 118:203-214(1993) [PubMed: 8104141] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71866 Genomic DNA. Translation: CAA50701.1. AE013599 Genomic DNA. Translation: AAF58591.1. AY069810 mRNA. Translation: AAL39955.1.
PIR	S42582.
RefSeq	NP_536733.1.
UniGene	Dm.7739
3D structure databases
HSSP	HSSP built from PDB template 1LNZ based on UniProtKB P20964.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32234.
Genome annotation databases
Ensembl	CG8340. Drosophila melanogaster. [Contig view]
GeneID	36288.
KEGG	dme:Dmel_CG8340.
NMPDR	fig|7227.3.peg.4742.
Organism-specific databases
FlyBase	FBgn0010339. 128up.
Phylogenomic databases
HOGENOM	P32234.
Gene expression databases
ArrayExpress	P32234.
GermOnline	CG8340. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006074. GTP1-OBG_CS. IPR006073. GTP1_OBG. IPR002917. MMR_HSR1_GTP_bd. IPR005225. Small_GTP_bd. IPR004095. TGS. [Graphical view]
Pfam	PF01926. MMR_HSR1. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
PRINTS	PR00326. GTP1OBG.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS00905. GTP1_OBG. 1 hit. [Graphical view]
ProDom	P32234. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

128UP_DROME

Accession

Primary (citable) accession number: P32234
Secondary accession number(s): Q9V648

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	March 29, 2005
Last modified:	July 22, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project