Reviewed, UniProtKB/Swiss-Prot P32912 (VAM7_YEAST)

Names and origin

Protein names

Recommended name:
    Vacuolar morphogenesis protein 7

Gene names

Name:	VAM7
Ordered Locus Names:	YGL212W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.
Subunit structure	Possibly multimeric. Associates with VAM3.
Subcellular location	Vacuole.
Miscellaneous	Present with 2360 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 PX (phox homology) domain. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Coiled coil
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Traceable author statement. Source: SGD vacuole fusion, non-autophagic Inferred from direct assay. Source: SGD vesicle fusion Traceable author statement. Source: SGD
Cellular component	fungal-type vacuole membrane Inferred from direct assay. Source: SGD
Molecular function	SNAP receptor activity Traceable author statement. Source: SGD phosphatidylinositol 3-phosphate binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BSP1	Q06604	1	EBI-20232,EBI-37047
HSV2	P50079	1	EBI-20232,EBI-23505
YIF1	P53845	1	EBI-20232,EBI-28230
YIP1	P53039	1	EBI-20232,EBI-25295

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 316

316

Vacuolar morphogenesis protein 7

Regions

Domain

1 – 124

124

PX

Domain

250 – 312

63

t-SNARE coiled-coil homology

Coiled coil

168 – 186

19

Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

316

Helix Strand Turn

Details...

Beta strand

16 – 22

7

Beta strand

27 – 32

6

Beta strand

38 – 40

3

Helix

42 – 56

15

Helix

82 – 89

8

Helix

92 – 100

9

Helix

107 – 110

4

Helix

112 – 117

6

Sequences

	Sequence		Length	Mass (Da)
	P32912-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 2F992AEC0ACBC8FE	FASTA	316	36,711
	10 20 30 40 50 60 MAANSVGKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT RLERDVGSTI 70 80 90 100 110 120 PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY NDRFDSRWRD TKIAQDFLQL 130 140 150 160 170 180 SKPNVSQEKS QQHLETADEV GWDEMIRDIK LDLDKESDGT PSVRGALRAR TKLHKLRERL 190 200 210 220 230 240 EQDVQKKSLP STEVTRRAAL LRSLLKECDD IGTANIAQDR GRLLGVATSD NSSTTEVQGR 250 260 270 280 290 300 TNNDLQQGQM QMVRDQEQEL VALHRIIQAQ RGLALEMNEE LQTQNELLTA LEDDVDNTGR 310 RLQIANKKAR HFNNSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. II. VAM7, a gene for regulating morphogenic assembly of the vacuoles." Wada Y., Anraku Y. J. Biol. Chem. 267:18671-18675(1992) [PubMed: 1526999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YWH019-7A.
[2]	"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes." Feuermann M., Simeonava L., Souciet J.-L., Potier S. Yeast 13:475-477(1997) [PubMed: 9153757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames." Kail M., Juettner E., Vaux D. Yeast 12:799-807(1996) [PubMed: 8813766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking." Sato T.K., Darsow T., Emr S.D. Mol. Cell. Biol. 18:5308-5319(1998) [PubMed: 9710615] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D11379 Genomic DNA. Translation: BAA01977.1.
U33754 Genomic DNA. Translation: AAC49494.1.
Z72734 Genomic DNA. Translation: CAA96928.1.

PIR

S31263.

RefSeq

NP_011303.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KMD	NMR	-	A	8-124	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1722N.

IntAct

P32912.

Genome annotation databases

Ensembl

YGL212W. Saccharomyces cerevisiae. [Contig view]

GeneID

852660.

GenomeReviews

Gene locus YGL212W in contig Y13135_GR.

KEGG

sce:YGL212W.

NMPDR

fig|4932.3.peg.2406.

Organism-specific databases

CYGD

YGL212w.

SGD

S000003180. VAM7.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P32912.

Gene expression databases

GermOnline

YGL212W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001683. PX.
IPR000727. T_SNARE.
[Graphical view]

Gene3D

G3DSA:3.30.1520.10. PX. 1 hit.

Pfam

PF00787. PX. 1 hit.
PF05739. SNARE. 1 hit.
[Graphical view]

SMART

SM00312. PX. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]

PROSITE

PS50195. PX. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProDom

P32912.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

P32912.

ProtoNet

Search...

Entry information

Entry name

VAM7_YEAST

Accession

Primary (citable) accession number: P32912

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	September 2, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)