Reviewed, UniProtKB/Swiss-Prot P33304 (AFR1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein AFR1

Gene names

Name:	AFR1
Ordered Locus Names:	YDR085C
ORF Names:	D4471

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation.
Induction	By pheromone (alpha-factor).
Sequence similarities	To yeast YER158C.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell morphogenesis during conjugation Ref.1 Inferred from mutant phenotype. Source: SGD pheromone-dependent signal transduction during conjugation with cellular fusion Inferred from genetic interaction. Source: SGD regulation of G-protein coupled receptor protein signaling pathway Inferred from mutant phenotype. Source: SGD
Cellular component	mating projection base Inferred from direct assay. Source: SGD
Molecular function	receptor signaling protein activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 620	620	Protein AFR1
Amino acid modifications
	Modified residue	22	1	Phosphoserine
	Modified residue	472	1	Phosphoserine
	Modified residue	526	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P33304-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: A9ABFBC9A26E62E5	FASTA	620	71,364
	10 20 30 40 50 60 MEGSYLSAQE NQPIPERLIP RSNSTSNLFA LSSTFSKLNV RNDADYNYSN PNKKRHIYSG 70 80 90 100 110 120 EIDCRSVTAA RKFPVRSCSM TAAQQRKRTA LFTVRERNSY HEGFNNDQDY VSQYQKPQYT 130 140 150 160 170 180 FGVYKELTPY QLQRSKMKRS FQFPNGEIYK PKLDGKCTHS LKKPELNSRD SSLFKFSEKK 190 200 210 220 230 240 GRNLSKDFVG PHNGTSVIHI PPNDTGYGVN SLELNTSVPS TIKSSVSSTS PISAVNTLTS 250 260 270 280 290 300 LPESQTDDDD GYENKTVTIS YCFENTVNEK HGSHIEKLDL STKEKTKPTT NSGLFDRKKK 310 320 330 340 350 360 TILGTEKYRC IKSQSKLKLG SVLKKLWRTS GNSNTKHGKK DTKRRRIPID DMVTHSDGNS 370 380 390 400 410 420 EAENDIELMD ANLDGIEFDD DETLMDTDSI FDDLLSKEND KYDLRRRQLE IRQKLHETSH 430 440 450 460 470 480 NDDGKVSFRD TEKHNVNEGL IDKTIIEEFS KLGEYIIDTR NQPPPRSSKR PSLDDNESAR 490 500 510 520 530 540 YFYNISTDLR QSLSGPISLP MHVGNDMVNR LRNDWEYIRF EDRRNSLPDS SFDKVETPPK 550 560 570 580 590 600 PIKKDVRFAK EVCLASTWSS NAYERANPEF IMNRHRLLWM MKVHPSMNSA MNEIKLELNS 610 620 YKKNEMVVHE NSKCFTHYLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation." Konopka J.B. Mol. Cell. Biol. 13:6876-6888(1993) [PubMed: 8413281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, MASS SPECTROMETRY.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472 AND SER-526, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46796 Genomic DNA. Translation: CAA86807.1. L21702 Unassigned DNA. Translation: AAC37365.1. X82086 Genomic DNA. Translation: CAA57614.1. Z74381 Genomic DNA. Translation: CAA98905.1. AY723775 Genomic DNA. Translation: AAU09692.1.
PIR	S48772.
RefSeq	NP_010370.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:824N.
IntAct	P33304.
Genome annotation databases
Ensembl	YDR085C. Saccharomyces cerevisiae. [Contig view]
GeneID	851658.
GenomeReviews	Gene locus YDR085C in contig Z71256_GR.
KEGG	sce:YDR085C.
NMPDR	fig|4932.3.peg.1116.
Organism-specific databases
CYGD	YDR085c.
SGD	S000002492. AFR1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P33304.
Gene expression databases
ArrayExpress	P33304.
GermOnline	YDR085C. Saccharomyces cerevisiae.
Family and domain databases
ProDom	P33304. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P33304.
ProtoNet	Search...

Entry information

Entry name

AFR1_YEAST

Accession

Primary (citable) accession number: P33304

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	July 22, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)