Reviewed, UniProtKB/Swiss-Prot P34905 (MTB1_BREBE)

Names and origin

Protein names

Recommended name:
    Modification methylase BbvI
      Short name=M.BbvI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase BbvI

Gene names

Name:

bbvIM

Organism

Brevibacillus brevis (Bacillus brevis)

Taxonomic identifier

1393 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Brevibacillus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GCWGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the BbvI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 374	374	Modification methylase BbvI
Sites
	Active site	92	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34905-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 538127C7B761DC01	FASTA	374	42,350
	10 20 30 40 50 60 MKFRKGELFC GPGGLALGAK EAKYMHPETG EVFEFEHAWA NDIDEWACET FRTNICPDRP 70 80 90 100 110 120 DSVVCGDVRE LDIKSLGEKF GEIDAFTFGF PCNDYSIVGE HKGMEGNYGP LYSYGVKILN 130 140 150 160 170 180 EYNPLVFIAE NVGGLQSANE GKAFLGILND LASAGKYGYK LVPHLYKFEE YGVPQRRHRI 190 200 210 220 230 240 IIVGIRKDQD VAFRVPEPTH KEKYRTASEA LADIPEDALN HEFTRHKKKV VEMLNHIAPG 250 260 270 280 290 300 GNAWSESIPE ELRLNVKKVR MSQIYRRLHP DQPSYTVTGS GGGGTHGYHW EEPRALTNRE 310 320 330 340 350 360 RARLQTFPDD YEFIGKKEMV RKQIGMAVPP DGAKIILEAV LKTFARIEYP SINSKWDFES 370 VSAEQVIEEV QEIM

References

[1]	Barsomian J.M., Wilson G.G. Submitted (FEB-1994) to UniProtKB Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTB1_BREBE

Accession

Primary (citable) accession number: P34905

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	September 2, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)