Reviewed, UniProtKB/Swiss-Prot P35342 (AA3R_SHEEP)

Names and origin

Protein names

Recommended name:
    Adenosine A3 receptor

Gene names

Name:

ADORA3

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Most abundant in lung, spleen and pineal gland. Moderate expression in brain, kidney and testis.
Post-translational modification	Phosphorylation on Thr-315 and Ser-316 may be crucial for rapid desensitization. Phosphorylation on Thr-315 may be necessary for phosphorylation on Ser-316 to occur By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 317	317	Adenosine A3 receptor
Regions
	Topological domain	1 – 14	14	Extracellular Potential
	Transmembrane	15 – 37	23	1 Potential
	Topological domain	38 – 48	11	Cytoplasmic Potential
	Transmembrane	49 – 72	24	2 Potential
	Topological domain	73 – 84	12	Extracellular Potential
	Transmembrane	85 – 106	22	3 Potential
	Topological domain	107 – 126	20	Cytoplasmic Potential
	Transmembrane	127 – 148	22	4 Potential
	Topological domain	149 – 176	28	Extracellular Potential
	Transmembrane	177 – 197	21	5 Potential
	Topological domain	198 – 230	33	Cytoplasmic Potential
	Transmembrane	231 – 254	24	6 Potential
	Topological domain	255 – 260	6	Extracellular Potential
	Transmembrane	261 – 283	23	7 Potential
	Topological domain	284 – 317	34	Cytoplasmic Potential
Amino acid modifications
	Modified residue	315	1	Phosphothreonine By similarity
	Modified residue	316	1	Phosphoserine By similarity
	Lipidation	302	1	S-palmitoyl cysteine Potential
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Disulfide bond	83 ↔ 165		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35342-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: CE362B80F9B8BE56	FASTA	317	36,141
	10 20 30 40 50 60 MPVNSTAVSW TSVTYITVEI LIGLCAIVGN VLVIWVVKLN PSLQTTTFYF IVSLALADIA 70 80 90 100 110 120 VGVLVMPLAI VISLGVTIHF YSCLFMTCLM LIFTHASIMS LLAIAVDRYL RVKLTVRYRR 130 140 150 160 170 180 VTTQRRIWLA LGLCWLVSFL VGLTPMFGWN MKLSSADENL TFLPCRFRSV MRMDYMVYFS 190 200 210 220 230 240 FFLWILVPLV VMCAIYFDIF YIIRNRLSQS FSGSRETGAF YGREFKTAKS LLLVLFLFAL 250 260 270 280 290 300 CWLPLSIINC ILYFDGQVPQ TVLYLGILLS HANSMMNPIV YAYKIKKFKE TYLLILKACV 310 MCQPSKSMDP STEQTSE

References

[1]

"Molecular cloning and functional expression of a sheep A3 adenosine receptor with widespread tissue distribution." Linden J., Taylor H.E., Robeva A.S., Tucker A.L., Stehle J.H., Rivkees S.A., Fink J.S., Reppert S.M. Mol. Pharmacol. 44:524-532(1993) [PubMed: 8396714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary pars tuberalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S65334 mRNA. Translation: AAB28171.1.
RefSeq	NP_001009775.1.
UniGene	Oar.514
3D structure databases
HSSP	HSSP built from PDB template 1MMH based on UniProtKB P29274.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
GeneID	443330.
Phylogenomic databases
HOVERGEN	P35342.
Family and domain databases
InterPro	IPR000466. Adeno_A3_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00555. ADENOSINEA3R. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P35342. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AA3R_SHEEP

Accession

Primary (citable) accession number: P35342

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	September 2, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)