Reviewed, UniProtKB/Swiss-Prot P35383 (P2RY2_MOUSE)

Names and origin

Protein names

Recommended name:
    P2Y purinoceptor 2
      Short name=P2Y2
Alternative name(s):
    P2U purinoceptor 1
      Short name=P2U1
    ATP receptor
    Purinergic receptor

Gene names

Name:	P2ry2
Synonyms:	P2ru1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Spleen, testis, kidney, liver, lung, heart and brain.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 373	373	P2Y purinoceptor 2
Regions
	Topological domain	1 – 32	32	Extracellular Potential
	Transmembrane	33 – 59	27	1 Potential
	Topological domain	60 – 70	11	Cytoplasmic Potential
	Transmembrane	71 – 93	23	2 Potential
	Topological domain	94 – 110	17	Extracellular Potential
	Transmembrane	111 – 129	19	3 Potential
	Topological domain	130 – 152	23	Cytoplasmic Potential
	Transmembrane	153 – 172	20	4 Potential
	Topological domain	173 – 194	22	Extracellular Potential
	Transmembrane	195 – 220	26	5 Potential
	Topological domain	221 – 246	26	Cytoplasmic Potential
	Transmembrane	247 – 269	23	6 Potential
	Topological domain	270 – 287	18	Extracellular Potential
	Transmembrane	288 – 309	22	7 Potential
	Topological domain	310 – 373	64	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Disulfide bond	106 ↔ 183		By similarity
Experimental info
	Mutagenesis	107	1	K → I: No effect on receptor activation
	Mutagenesis	110	1	R → L: No effect on receptor activation
	Mutagenesis	262	1	H → L: Decrease in receptor activation
	Mutagenesis	265	1	R → L: Decrease in receptor activation
	Mutagenesis	289	1	K → I: No effect on receptor activation
	Mutagenesis	292	1	R → L: Decrease in receptor activation
	Sequence conflict	17	1	E → D in AAB50735. Ref.2
	Sequence conflict	120	1	S → R in AAB50735. Ref.2
	Sequence conflict	125	1	T → N in AAB50735. Ref.2
	Sequence conflict	196	1	V → M in AAB50735. Ref.2
	Sequence conflict	263	1	V → L in BAB23746 and BAB30719. Ref.3
	Sequence conflict	355	1	D → N in AAB50735. Ref.2
	Sequence conflict	369 – 371	3	KDI → PYV in AAB50735. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35383-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 590BBE502E41B3AE	FASTA	373	42,175
	10 20 30 40 50 60 MAADLEPWNS TINGTWEGDE LGYKCRFNED FKYVLLPVSY GVVCVLGLCL NVVALYIFLC 70 80 90 100 110 120 RLKTWNASTT YMFHLAVSDS LYAASLPLLV YYYARGDHWP FSTVLCKLVR FLFYTNLYCS 130 140 150 160 170 180 ILFLTCISVH RCLGVLRPLH SLRWGRARYA RRVAAVVWVL VLACQAPVLY FVTTSVRGTR 190 200 210 220 230 240 ITCHDTSARE LFSHFVAYSS VMLGLLFAVP FSVILVCYVL MARRLLKPAY GTTGGLPRAK 250 260 270 280 290 300 RKSVRTIALV LAVFALCFLP FHVTRTLYYS FRSLDLSCHT LNAINMAYKI TRPLASANSC 310 320 330 340 350 360 LDPVLYFLAG QRLVRFARDA KPPTEPTPSP QARRKLGLHR PNRTVRKDLS VSSDDSRRTE 370 STPAGSETKD IRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of an ATP receptor from mouse neuroblastoma cells." Lustig K.D., Shiau A.K., Brake A.J., Julius D. Proc. Natl. Acad. Sci. U.S.A. 90:5113-5117(1993) [PubMed: 7685114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells." Enomoto K., Furuya K., Moore R.C., Yamagishi S., Oka T., Maeno T. Biol. Signals 5:9-21(1996) [PubMed: 8739319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head and Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[5]	"Site-directed mutagenesis of P2U purinoceptors. Positively charged amino acids in transmembrane helices 6 and 7 affect agonist potency and specificity." Erb L., Garrad R.C., Wang Y., Quinn T., Turner J.T., Weisman G.A. J. Biol. Chem. 270:4185-4188(1995) [PubMed: 7876172] [Abstract] Cited for: MUTAGENESIS OF LYS-107; ARG-110; HIS-262; ARG-265; LYS-289 AND ARG-292.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L14751 mRNA. Translation: AAA39871.1. S83099 mRNA. Translation: AAB50735.1. AK005013 mRNA. Translation: BAB23746.1. AK017378 mRNA. Translation: BAB30719.1. BC006613 mRNA. Translation: AAH06613.1.
PIR	A47556.
RefSeq	NP_032799.2.
UniGene	Mm.3000
3D structure databases
HSSP	HSSP built from PDB template 1DDD based on UniProtKB P34996.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35383.
Genome annotation databases
Ensembl	ENSMUSG00000032860. Mus musculus. [Contig view]
GeneID	18442.
KEGG	mmu:18442.
Organism-specific databases
MGI	MGI:105107. P2ry2.
Phylogenomic databases
HOGENOM	P35383.
HOVERGEN	P35383.
Gene expression databases
ArrayExpress	P35383.
CleanEx	MM_P2RY2.
GermOnline	ENSMUSG00000032860. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR002286. P2_purnocptor. IPR000356. P2U_purnocptor. [Graphical view]
PANTHER	PTHR19264:SF155. P2U_purnocptor. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00594. P2Y2PRNOCPTR. PR01157. P2YPURNOCPTR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P35383. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

P2RY2_MOUSE

Accession

Primary (citable) accession number: P35383
Secondary accession number(s): O09031, Q9CPZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	September 2, 2008
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)