Reviewed, UniProtKB/Swiss-Prot P36048 (SN114_YEAST)

Names and origin

Protein names

Recommended name:
    114 kDa U5 small nuclear ribonucleoprotein component
Alternative name(s):
    GIN10 protein

Gene names

Name:	SNU114
Synonyms:	GIN10
Ordered Locus Names:	YKL173W
ORF Names:	YKL637

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1008 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
Subcellular location	Nucleus.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	assembly of spliceosomal tri-snRNP Inferred from mutant phenotype. Source: SGD generation of catalytic spliceosome for first transesterification step Inferred from genetic interaction. Source: SGD spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) Inferred from mutant phenotype. Source: SGD
Cellular component	U4/U6 x U5 tri-snRNP complex Inferred from direct assay. Source: SGD snRNP U5 Inferred from direct assay. Source: SGD
Molecular function	GTPase activity Ref.4 Inferred from mutant phenotype. Source: SGD U5 snRNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1008	1008	114 kDa U5 small nuclear ribonucleoprotein component		PRO_0000091565
Regions
	Nucleotide binding	140 – 147	8	GTP By similarity
	Nucleotide binding	214 – 218	5	GTP By similarity
	Nucleotide binding	268 – 271	4	GTP By similarity
Amino acid modifications
	Modified residue	85	1	Phosphoserine
	Modified residue	88	1	Phosphothreonine

Sequences

	Sequence		Length	Mass (Da)
	P36048-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 5CE30CEFF154D013	FASTA	1,008	114,041
	10 20 30 40 50 60 MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK 70 80 90 100 110 120 ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL QEHTIFTQLK KNIPKTRYNR 130 140 150 160 170 180 DYMLSMANIP ERIINVGVIG PLHSGKTSLM DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN 190 200 210 220 230 240 LKQEIDRGLS IKLNGSTLLC TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID 250 260 270 280 290 300 VVEGVTFVVE QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK 310 320 330 340 350 360 GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG SVYYHKGNFR 370 380 390 400 410 420 TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL RSNFRVNLSQ EALQYDPQPF 430 440 450 460 470 480 LKHVLQLIFR QQTGLVDAIT RCYQPFELFD NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG 490 500 510 520 530 540 AEWSLVRIYS GLLKRGDTVR ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE 550 560 570 580 590 600 EIGLLGGRYV YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE 610 620 630 640 650 660 AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL YMDCLLYDLR 670 680 690 700 710 720 ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE ENLPGLSISV AAEPMDSKMI 730 740 750 760 770 780 QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI LRTEYGWDSL ASRNVWSFYN GNVLINDTLP 790 800 810 820 830 840 DEISPELLSK YKEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP 850 860 870 880 890 900 LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL 910 920 930 940 950 960 EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF IPKLKPAPIN 970 980 990 1000 SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ LRENGLVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Akada R., Yamamoto J., Yamashita I. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 823-846. Strain: ATCC 26786 / X2180-1A.
[4]	"An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2." Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R. EMBO J. 16:4092-4106(1997) [PubMed: 9233818] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND THR-88, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z26878 Genomic DNA. Translation: CAA81514.1. Z28173 Genomic DNA. Translation: CAA82015.1. D28149 Genomic DNA. Translation: BAA05682.1.
PIR	S38003.
RefSeq	NP_012748.1.
3D structure databases
HSSP	HSSP built from PDB template 1N0U based on UniProtKB P32324.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:759N.
IntAct	P36048.
Proteomic databases
PeptideAtlas	P36048.
Genome annotation databases
Ensembl	YKL173W. Saccharomyces cerevisiae. [Contig view]
GeneID	853681.
GenomeReviews	Gene locus YKL173W in contig Y13137_GR.
KEGG	sce:YKL173W.
NMPDR	fig|4932.3.peg.3726.
Organism-specific databases
CYGD	YKL173w.
SGD	S000001656. SNU114.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P36048.
Gene expression databases
ArrayExpress	P36048.
GermOnline	YKL173W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000795. ProtSyn_GTP_bd. IPR014721. Ribosomal_S5_D2-type_fold. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
PROSITE	PS00301. EFACTOR_GTP. False negative. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
LinkHub	P36048.
NextBio	974643.

Entry information

Entry name

SN114_YEAST

Accession

Primary (citable) accession number: P36048

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 4, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)