Reviewed, UniProtKB/Swiss-Prot P36108 (DID4_YEAST)

Names and origin

Protein names

Recommended name:
    DOA4-independent degradation protein 4
Alternative name(s):
    Vacuolar protein-sorting-associated protein 2
    Vacuolar protein-targeting protein 14

Gene names

Name:	DID4
Synonyms:	CHM2, GRD7, REN1, VPL2, VPS2, VPT14
Ordered Locus Names:	YKL002W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB).
Subunit structure	A component of the endosomal sorting required for transport complex III (ESCRT-III), which consists of DID4, SNF7, VPS20 and VPS24.
Subcellular location	Cytoplasm. Endosome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the SNF7 family.
Sequence caution	The sequence CAA81834.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Membrane
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	late endosome to vacuole transport Ref.4 Inferred from physical interaction. Source: SGD protein retention in Golgi apparatus Inferred from mutant phenotype. Source: SGD ubiquitin-dependent protein catabolic process via the multivesicular body pathway Inferred by curator. Source: SGD
Cellular component	ESCRT III complex Ref.4 Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG17	Q06410	1	EBI-26574,EBI-30856
JSN1	P47135	1	EBI-26574,EBI-9422
MOB2	P43563	1	EBI-26574,EBI-11125
NOT2	P06100	1	EBI-26574,EBI-12153
STD1	Q02794	1	EBI-26574,EBI-18344

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 232

232

DOA4-independent degradation protein 4

PRO_0000211477

Regions

Coiled coil

14 – 97

84

Potential

Amino acid modifications

Modified residue

107

1

Phosphoserine

Modified residue

211

1

Phosphoserine

Secondary structure

1

.

.

.

.

.

.

.

.

232

Helix Strand Turn

Details...

Helix

188 – 191

4

Helix

197 – 200

4

Beta strand

201 – 204

4

Helix

219 – 229

11

Sequences

	Sequence		Length	Mass (Da)
	P36108-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 200D07DB4C45DCBC	FASTA	232	26,291
	10 20 30 40 50 60 MSLFEWVFGK NVTPQERLKK NQRALERTQR ELEREKRKLE LQDKKLVSEI KKSAKNGQVA 70 80 90 100 110 120 AAKVQAKDLV RTRNYIQKFD NMKAQLQAIS LRIQAVRSSD QMTRSMSEAT GLLAGMNRTM 130 140 150 160 170 180 NLPQLQRISM EFEKQSDLMG QRQEFMDEAI DNVMGDEVDE DEEADEIVNK VLDEIGVDLN 190 200 210 220 230 SQLQSTPQNL VSNAPIAETA MGIPEPIGAG SEFHGNPDDD LQARLNTLKK QT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast." Davis C.A., Grate L., Spingola M., Ares M. Jr. Nucleic Acids Res. 28:1700-1706(2000) [PubMed: 10734188] [Abstract] Cited for: IDENTIFICATION OF INTRON.
[3]	"CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins." Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M. J. Cell Sci. 114:2395-2404(2001) [PubMed: 11559748] [Abstract] Cited for: FUNCTION.
[4]	"Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting." Babst M., Katzmann D.J., Estepa-Sabal E.J., Meerloo T., Emr S.D. Dev. Cell 3:271-282(2002) [PubMed: 12194857] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH VPS24, SUBCELLULAR LOCATION.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107 AND SER-211, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z28002 Genomic DNA. Translation: CAA81834.1. Sequence problems.

PIR

S37812.

RefSeq

NP_012924.2.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2V6X	X-ray	1.98	B	183-232	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1916N.

IntAct

P36108.

Genome annotation databases

Ensembl

YKL002W. Saccharomyces cerevisiae. [Contig view]

GeneID

853868.

GenomeReviews

Gene locus YKL002W in contig Y13137_GR.

KEGG

sce:YKL002W.

NMPDR

fig|4932.3.peg.3910.

Organism-specific databases

CYGD

YKL002w.

SGD

S000001485. DID4.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P36108.

Gene expression databases

GermOnline

YKL002W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005024. Snf7.
[Graphical view]

Pfam

PF03357. Snf7. 1 hit.
[Graphical view]

BLOCKS

Search...

ProtoNet

Search...

Other Resources

LinkHub

P36108.

NextBio

975133.

Entry information

Entry name

DID4_YEAST

Accession

Primary (citable) accession number: P36108

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 17, 2006
Last modified:	November 4, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)