Reviewed, UniProtKB/Swiss-Prot P36216 (MTC1_PBCVI)

Names and origin

Protein names

Recommended name:
    Modification methylase CviJI
      Short name=M.CviJI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase CviJI

Gene names

Name:

CVIJIM

Organism

Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)

Taxonomic identifier

46019 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus

Virus host

Chlorella [TaxID: 3071]

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence RGCY, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the CviJI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 367	367	Modification methylase CviJI
Sites
	Active site	73	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36216-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 15BD56B0354E2ECC	FASTA	367	41,861
	10 20 30 40 50 60 MSFRTLELFA GIAGISHGLR GISTPVAFVE INEDAQKFLK TKFSDASVFN DVTKFTKSDF 70 80 90 100 110 120 PEDIDMITAG FPCTGFSIAG SRTGFEHKES GLFADVVRIT EEYKPKIVFL ENSHMLSHTY 130 140 150 160 170 180 NLDVVVKKMD EIGYFCKWVT CRASIIGAHH QRHRWFCLAI RKDYEPEEII VSVNATKFDW 190 200 210 220 230 240 ENNEPPCQVD NKSYENSTLV RLAGYSVVPD QIRYAFTGLF TGDFESSWKT TLTPGTIIGT 250 260 270 280 290 300 EHKKMKGTYD KVINGYYEND VYYSFSRKEV HRAPLNISVK PRDIPEKHNG KTLVDREMIK 310 320 330 340 350 360 KYWCTPCASY GTATAGCNVL TDRQSHALPT QVRFSYRGVC GRHLSGIWCA WLMGYDQEYL GYLVQYD

References

[1]	"Cloning and sequencing the cytosine methyltransferase gene M. CviJI from Chlorella virus IL-3A." Shields S.L., Burbank D.E., Grabherr R., van Etten J.L. Virology 176:16-24(1990) [PubMed: 2158687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A." Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M. Nucleic Acids Res. 24:2463-2469(1996) [PubMed: 8692682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 357-367.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M27265 Genomic DNA. Translation: AAA88826.1. U09001 Genomic DNA. Translation: AAC55063.1.
PIR	A46355.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3353. M.CviJI.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProDom	P36216. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTC1_PBCVI

Accession

Primary (citable) accession number: P36216

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	September 2, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)