Reviewed, UniProtKB/Swiss-Prot P36428 (SYA_ARATH)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase, mitochondrial
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	ALATS
Synonyms:	ALARS
Ordered Locus Names:	At1g50200
ORF Names:	F14I3.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1003 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion. Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: P36428-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic (identifier: P36428-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 49	49	Mitochondrion
	Chain	50 – 1003	954	Alanyl-tRNA synthetase, mitochondrial
Natural variations
	Alternative sequence	1 – 48	48	Missing in isoform Cytoplasmic.
Experimental info
	Sequence conflict	194	1	G → R in CAA80380 and CAA80381. Ref.1
	Sequence conflict	731	1	V → A in CAA80380 and CAA80381. Ref.1
	Sequence conflict	910	1	R → Q in CAA80380 and CAA80381. Ref.1
	Sequence conflict	985	1	S → F in CAA73145. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Mitochondrial [UniParc]. Last modified June 20, 2002. Version 3. Checksum: 0A85DA940C5BB308	FASTA	1,003	110,489
	10 20 30 40 50 60 MRLVKAASLL ISSTKPPSRV FYSSHLRRPF FSHFRFSSSS STSSSVAVMP GSEPSETQWP 70 80 90 100 110 120 AKRVRDTYVD FFRGKGHKFW PSSPVVPHND PTLLFANAGM NQYKPIFLGT ADPNTELSKL 130 140 150 160 170 180 SRACNTQKCI RAGGKHNDLD DVGKDTYHHT FFEMLGNWSF GDYFKKEAIE WAWELLTKVY 190 200 210 220 230 240 GLPTDRIYAT YFGGDEKAGL QPDNEARDIW LKVLPSGRVL PFGCKDNFWE MGDTGPCGPC 250 260 270 280 290 300 TEIHYDRIGN RDAASLVNND DPTCLEIWNL VFIQFNRESD GSLKPLPAKH VDTGMGFERL 310 320 330 340 350 360 TSVLQNKMSN YDTDVFMPIF DDIQKATGAR PYSGKVGPED VDRVDMAYRV VADHIRTLSF 370 380 390 400 410 420 AIADGSRPGN EGREYVLRRI LRRAVRYGKE ILKAEEGFFN GLVSSVIRVM GDVFTELKEH 430 440 450 460 470 480 EKKITDIIKE EEASFCKTLA KGIEKFRKAG QAVQGNTLSG DDAFILWDTY GFPLDLTQLM 490 500 510 520 530 540 AEERGLLVDV DGFNKAMEEA RERSRSAQNK QAGGAIVMDA DATSTLHKAG VSATDDSFKY 550 560 570 580 590 600 IWFQDHESEL KAIYTGSTFL ESSAASDNVG LVLGSTSFYA EQGGQIFDTG LIEGSFGTFN 610 620 630 640 650 660 VCNVQIFGGF VLHIGYLSKE TGEVSVGDKV ICKVDYERRK LIAPNHTCTH MLNYALKEVL 670 680 690 700 710 720 GDHIDQKGSI VLPEKLRFDF SHGKPVDPED LRRIESIVNK QIKDELDVFS KEAVLSEAKR 730 740 750 760 770 780 IKGLRAVFGE VYPDPVRVVS IGRKVEDLLA DPENNEWSLL SSEFCGGTHI TNTREAKAFA 790 800 810 820 830 840 LLSEEGIAKG IRRVTAVTTE CAFDALNAAS LLEREVEDAS RAEGSALEKK VSALKSRVDA 850 860 870 880 890 900 AIIPAAKKAD IRTKIASLQN EVRKAQKKIA EQNLKKSVKL ATEAAESAAS DGKTFCIIQL 910 920 930 940 950 960 DVGLDAAAVR EAVSKVMEKK GMSIMVFSTD ESTNKAVVCA GVPEKSDQFK PLDVTEWLTT 970 980 990 1000 ALGPLKGRCG KGKGGLASGQ GTDASQVQAA LDMASSFASM KLN
	Isoform Cytoplasmic [UniParc]. Checksum: 78F291B2EFA2662B		955	105,172
	10 20 30 40 50 60 MPGSEPSETQ WPAKRVRDTY VDFFRGKGHK FWPSSPVVPH NDPTLLFANA GMNQYKPIFL 70 80 90 100 110 120 GTADPNTELS KLSRACNTQK CIRAGGKHND LDDVGKDTYH HTFFEMLGNW SFGDYFKKEA 130 140 150 160 170 180 IEWAWELLTK VYGLPTDRIY ATYFGGDEKA GLQPDNEARD IWLKVLPSGR VLPFGCKDNF 190 200 210 220 230 240 WEMGDTGPCG PCTEIHYDRI GNRDAASLVN NDDPTCLEIW NLVFIQFNRE SDGSLKPLPA 250 260 270 280 290 300 KHVDTGMGFE RLTSVLQNKM SNYDTDVFMP IFDDIQKATG ARPYSGKVGP EDVDRVDMAY 310 320 330 340 350 360 RVVADHIRTL SFAIADGSRP GNEGREYVLR RILRRAVRYG KEILKAEEGF FNGLVSSVIR 370 380 390 400 410 420 VMGDVFTELK EHEKKITDII KEEEASFCKT LAKGIEKFRK AGQAVQGNTL SGDDAFILWD 430 440 450 460 470 480 TYGFPLDLTQ LMAEERGLLV DVDGFNKAME EARERSRSAQ NKQAGGAIVM DADATSTLHK 490 500 510 520 530 540 AGVSATDDSF KYIWFQDHES ELKAIYTGST FLESSAASDN VGLVLGSTSF YAEQGGQIFD 550 560 570 580 590 600 TGLIEGSFGT FNVCNVQIFG GFVLHIGYLS KETGEVSVGD KVICKVDYER RKLIAPNHTC 610 620 630 640 650 660 THMLNYALKE VLGDHIDQKG SIVLPEKLRF DFSHGKPVDP EDLRRIESIV NKQIKDELDV 670 680 690 700 710 720 FSKEAVLSEA KRIKGLRAVF GEVYPDPVRV VSIGRKVEDL LADPENNEWS LLSSEFCGGT 730 740 750 760 770 780 HITNTREAKA FALLSEEGIA KGIRRVTAVT TECAFDALNA ASLLEREVED ASRAEGSALE 790 800 810 820 830 840 KKVSALKSRV DAAIIPAAKK ADIRTKIASL QNEVRKAQKK IAEQNLKKSV KLATEAAESA 850 860 870 880 890 900 ASDGKTFCII QLDVGLDAAA VREAVSKVME KKGMSIMVFS TDESTNKAVV CAGVPEKSDQ 910 920 930 940 950 FKPLDVTEWL TTALGPLKGR CGKGKGGLAS GQGTDASQVQ AALDMASSFA SMKLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The same Arabidopsis gene encodes both cytosolic and mitochondrial alanyl-tRNA synthetases." Mireau H., Lancelin D., Small I. Plant Cell 8:1027-1039(1996) [PubMed: 8672889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CYTOPLASMIC AND MITOCHONDRIAL). Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CYTOPLASMIC). Strain: cv. Columbia.
[4]	"An Arabidopsis embryonic lethal mutant with reduced expression of alanyl-tRNA synthetase gene." Ge S.J., Yao X.L., Yang Z.X., Zhu Z.P. Cell Res. 8:119-134(1998) [PubMed: 9669027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 753-1003. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22673 mRNA. Translation: CAA80380.1. Z22673 mRNA. Translation: CAA80381.1. AC007980 Genomic DNA. Translation: AAD50044.1. Different initiation. BT002526 mRNA. Translation: AAO00886.1. BT008807 mRNA. Translation: AAP68246.1. Y12555 mRNA. Translation: CAA73145.1.
PIR	D96538.
RefSeq	NP_175439.2.
UniGene	At.1836
3D structure databases
ModBase	Search...
Proteomic databases
ProMEX	P36428.
Genome annotation databases
GeneID	841442.
GenomeReviews	Gene locus AT1G50200 in contig CT485782_GR.
KEGG	ath:AT1G50200.
Organism-specific databases
GeneFarm	2386.
TAIR	At1g50200.
Gene expression databases
ArrayExpress	P36428.
GermOnline	AT1G50200. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	P36428. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_ARATH

Accession

Primary (citable) accession number: P36428
Secondary accession number(s): O04159, Q8GUG2, Q9SX40

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 20, 2002
Last modified:	July 22, 2008
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)