Reviewed, UniProtKB/Swiss-Prot P38645 (BGLB_MICBI)

Names and origin

Protein names

Recommended name:
    Thermostable beta-glucosidase B
    EC=3.2.1.21
Alternative name(s):
    Gentiobiase
    Cellobiase
    Beta-D-glucoside glucohydrolase
    Amygdalase

Gene names

Name:

bglB

Organism

Microbispora bispora

Taxonomic identifier

2006 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Thermobispora

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
Subcellular location	Cytoplasm.
Miscellaneous	Shows greater activity against cellobiose than against aryl-beta-D-glucosides.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Biophysicochemical properties	Temperature dependence: Thermostable, retains about 70% of its activity at 60 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 473	473	Thermostable beta-glucosidase B
Sites
	Active site	196	1	Proton donor Potential
	Active site	378	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38645-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 8F2188F1C93007CA	FASTA	473	52,228
	10 20 30 40 50 60 MTESAMTSRA GRGRGADLVA AVVQGHAAAS DAAGDLSFPD GFIWGAATAA YQIEGAWRED 70 80 90 100 110 120 GRGLWDVFSH TPGKVASGHT GDIACDHYHR YADDVRLMAG LGDRVYRFSV AWPRIVPDGS 130 140 150 160 170 180 GPVNPAGLDF YDRLVDELLG HGITPYPTLY HWDLPQTLED RGGWAARDTA YRFAEYALAV 190 200 210 220 230 240 HRRLGDRVRC WITLNEPWVA AFLATHRGAP GAADVPRFRA VHHLLLGHGL GLRLRSAGAG 250 260 270 280 290 300 QLGLTLSLSP VIEARPGVRG GGRRVDALAN RQFLDPALRG RYPEEVLKIM AGHARLGHPG 310 320 330 340 350 360 RDLETIHQPV DLLGVNYYSH VRLAAEGEPA NRLPGSEGIR FERPTAVTAW PGDRPDGLRT 370 380 390 400 410 420 LLLRLSRDYP GVGLIITENG AAFDDRADGD RVHDPERIRY LTATLRAVHD AIMAGADLRG 430 440 450 460 470 YFVWSVLDNF EWAYGYHKRG IVYVDYTTMR RIPRESALWY RDVVRRNGLR NGE

References

[1]

"Cloning, characterization, and nucleotide sequence of a gene encoding Microbispora bispora BglB, a thermostable beta-glucosidase expressed in Escherichia coli." Wright R.M., Yablonsky M.D., Shalita Z.P., Goyal A.K., Eveleigh D.E. Appl. Environ. Microbiol. 58:3455-3465(1992) [PubMed: 1482172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRRL 15568.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97265 Genomic DNA. Translation: AAA25311.1.
PIR	A48949.
3D structure databases
HSSP	HSSP built from PDB template 1GNX based on UniProtKB Q59976.
ModBase	Search...
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR017736. Glyco_hydro_1_beta-glucosidase. IPR013781. Glyco_hydro_sub_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProDom	P38645. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

BGLB_MICBI

Accession

Primary (citable) accession number: P38645

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 2, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)