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P38684

- TORR_ECOLI

UniProt

P38684 - TORR_ECOLI

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Protein
TorCAD operon transcriptional regulatory protein TorR
Gene
torR, b0995, JW0980
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Phosphorylated TorR activates the transcription of the torCAD operon by binding to four decameric boxes located in the torCAD promoter. Box1, 2 and 4 contain the DNA sequence 5'-CTGTTCATAT-3' and box3 contains the DNA sequence 5'-CCGTTCATCC-3'. Phosphorylated as well as unphosphorylated TorR negatively regulates its own expression by binding to box1 and 2.

GO - Molecular functioni

  1. DNA bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. phosphorelay response regulator activityInferred from direct assayi PubMed 8083154 Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. intracellular signal transductionInferred from direct assayi PubMed 8083154 Source: GOC
  2. regulation of transcription, DNA-templatedInferred from direct assayi PubMed 8083154 Source: EcoCyc
  3. transcription, DNA-templatedInferred from electronic annotationi Source: UniProtKB-KW
  1. phosphorelay response regulator activityInferred from direct assayi PubMed 8083154 Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:TORR-MONOMER.
ECOL316407:JW0980-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
TorCAD operon transcriptional regulatory protein TorR
Gene namesi
Name:torR
Ordered Locus Names:b0995, JW0980
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12615. torR.

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasmInferred from electronic annotationi Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Mutagenesisi53 – 531D → A: Loss of phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 230230TorCAD operon transcriptional regulatory protein TorR
PRO_0000081255

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Modified residuei53 – 5314-aspartylphosphate Inferred

Post-translational modificationi

Phosphorylated and dephosphorylated by TorS.

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriP38684.

Interactioni

Subunit structurei

Interacts with TorI. TorI binds to the effector domain of TorR. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torCAD promoter.1 Publication

Protein-protein interaction databases

DIPiDIP-11016N.
IntActiP38684. 4 interactions.
MINTiMINT-1283676.
STRINGi511145.b0995.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Beta strandi4 – 85
Helixi12 – 2413
Beta strandi28 – 347
Helixi35 – 4410
Beta strandi48 – 547
Beta strandi57 – 593
Helixi61 – 699
Beta strandi75 – 828
Helixi85 – 9410
Beta strandi97 – 1037
Helixi106 – 12015

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZGZX-ray1.80A/B/C/D1-122[»]
ProteinModelPortaliP38684.
SMRiP38684. Positions 2-225.

Miscellaneous databases

EvolutionaryTraceiP38684.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini1 – 117117Response regulatory

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
KOiK07772.
OMAiFERQHID.
OrthoDBiEOG6G4VQG.
PhylomeDBiP38684.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38684-1 [UniParc]FASTA

« Hide

MPHHIVIVED EPVTQARLQS YFTQEGYTVS VTASGAGLRE IMQNQSVDLI    50
LLDINLPDEN GLMLTRALRE RSTVGIILVT GRSDRIDRIV GLEMGADDYV 100
TKPLELRELV VRVKNLLWRI DLARQAQPHT QDNCYRFAGY CLNVSRHTLE 150
RDGEPIKLTR AEYEMLVAFV TNPGEILSRE RLLRMLSARR VENPDLRTVD 200
VLIRRLRHKL SADLLVTQHG EGYFLAADVC 230
Length:230
Mass (Da):26,233
Last modified:November 1, 1997 - v2
Checksum:iD1936404503FBA09
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflicti134 – 1341C → L in CAA63922. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94231 Genomic DNA. Translation: CAA63922.1.
U00096 Genomic DNA. Translation: AAC74080.1.
AP009048 Genomic DNA. Translation: BAA36137.1.
PIRiA64841.
RefSeqiNP_415515.1. NC_000913.3.
YP_489268.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74080; AAC74080; b0995.
BAA36137; BAA36137; BAA36137.
GeneIDi12932494.
946182.
KEGGiecj:Y75_p0968.
eco:b0995.
PATRICi32117215. VBIEscCol129921_1031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94231 Genomic DNA. Translation: CAA63922.1 .
U00096 Genomic DNA. Translation: AAC74080.1 .
AP009048 Genomic DNA. Translation: BAA36137.1 .
PIRi A64841.
RefSeqi NP_415515.1. NC_000913.3.
YP_489268.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZGZ X-ray 1.80 A/B/C/D 1-122 [» ]
ProteinModelPortali P38684.
SMRi P38684. Positions 2-225.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-11016N.
IntActi P38684. 4 interactions.
MINTi MINT-1283676.
STRINGi 511145.b0995.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74080 ; AAC74080 ; b0995 .
BAA36137 ; BAA36137 ; BAA36137 .
GeneIDi 12932494.
946182.
KEGGi ecj:Y75_p0968.
eco:b0995.
PATRICi 32117215. VBIEscCol129921_1031.

Organism-specific databases

EchoBASEi EB2499.
EcoGenei EG12615. torR.

Phylogenomic databases

eggNOGi COG0745.
HOGENOMi HOG000034819.
KOi K07772.
OMAi FERQHID.
OrthoDBi EOG6G4VQG.
PhylomeDBi P38684.

Enzyme and pathway databases

BioCyci EcoCyc:TORR-MONOMER.
ECOL316407:JW0980-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38684.
PROi P38684.

Gene expression databases

Genevestigatori P38684.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view ]
SMARTi SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52172. SSF52172. 1 hit.
PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes."
    Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C.
    J. Bacteriol. 176:5601-5606(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Erratum
    Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C.
    J. Bacteriol. 177:275-275(1995) [PubMed] [Europe PMC] [Abstract]
  3. "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
    Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.
    , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
    DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Binding of the TorR regulator to cis-acting direct repeats activates tor operon expression."
    Simon G., Jourlin C., Ansaldi M., Pascal M.-C., Chippaux M., Mejean V.
    Mol. Microbiol. 17:971-980(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  7. "Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli."
    Jourlin C., Ansaldi M., Mejean V.
    J. Mol. Biol. 267:770-777(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-53.
  8. "The TorR high-affinity binding site plays a key role in both torR autoregulation and torCAD operon expression in Escherichia coli."
    Ansaldi M., Simon G., Lepelletier M., Mejean V.
    J. Bacteriol. 182:961-966(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  9. "TorI, a response regulator inhibitor of phage origin in Escherichia coli."
    Ansaldi M., Theraulaz L., Mejean V.
    Proc. Natl. Acad. Sci. U.S.A. 101:9423-9428(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TORI.

Entry nameiTORR_ECOLI
AccessioniPrimary (citable) accession number: P38684
Secondary accession number(s): P77344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

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