Class E vacuolar protein-sorting machinery protein HSE1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P38753 (HSE1_YEAST)

Last modified November 25, 2008. Version 71. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Class E vacuolar protein-sorting machinery protein HSE1

Gene names

Name:	HSE1
Ordered Locus Names:	YHL002W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.
Subunit structure	Component of the ESCRT-0 complex composed of HSE1 and VPS27. Interacts with the ESCRT-I subunit VPS23, the UBP7 deubiquitinase and the E3 ligase RSP5.
Subcellular location	Endosome membrane; Peripheral membrane protein; Cytoplasmic side.
Miscellaneous	Present with 358 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the STAM family. Contains 1 SH3 domain. Contains 1 UIM (ubiquitin-interacting motif) repeat. Contains 1 VHS domain.

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Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Membrane
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	late endosome to vacuole transport Ref.2 Inferred from mutant phenotype. Source: SGD protein targeting to vacuole Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	ESCRT-0 complex Ref.2 Inferred from physical interaction. Source: SGD endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.2 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
VPS27	P40343	2	EBI-1382,EBI-20380

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 452

452

Class E vacuolar protein-sorting machinery protein HSE1

PRO_0000202885

Regions

Domain

15 – 145

131

VHS

Repeat

162 – 181

UIM

Domain

217 – 276

SH3

Amino acid modifications

Modified residue

162

Phosphoserine

Modified residue

365

Phosphoserine

Secondary structure

452

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P38753-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 026267836BBADF69
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FASTA

452

51,161

        10         20         30         40         50         60 
MSSSAIKIRN ALLKATDPKL RSDNWQYILD VCDLVKEDPE DNGQEVMSLI EKRLEQQDAN 

        70         80         90        100        110        120 
VILRTLSLTV SLAENCGSRL RQEISSKNFT SLLYALIESH SVHITLKKAV TDVVKQLSDS 

       130        140        150        160        170        180 
FKDDPSLRAM GDLYDKIKRK APYLVQPNVP EKHNMSTQAD NSDDEELQKA LKMSLFEYEK 

       190        200        210        220        230        240 
QKKLQEQEKE SAEVLPQQQQ QHQQQNQAPA HKIPAQTVVR RVRALYDLTT NEPDELSFRK 

       250        260        270        280        290        300 
GDVITVLEQV YRDWWKGALR GNMGIFPLNY VTPIVEPSKE EIEKEKNKEA IVFSQKTTID 

       310        320        330        340        350        360 
QLHNSLNAAS KTGNSNEVLQ DPHIGDMYGS VTPLRPQVTR MLGKYAKEKE DMLSLRQVLA 

       370        380        390        400        410        420 
NAERSYNQLM DRAANAHISP PVPGPALYAG MTHANNTPVM PPQRQSYQSN EYSPYPSNLP 

       430        440        450 
IQHPTNSANN TPQYGYDLGY SVVSQPPPGY EQ

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The Vps27p-Hse1p complex binds ubiquitin and mediates endosomal protein sorting." Bilodeau P.S., Urbanowski J.L., Winistorfer S.C., Piper R.C. Nat. Cell Biol. 4:534-539(2002) [PubMed: 12055639] [Abstract] Cited for: IDENTIFICATION IN THE ESCRT-0 COMPLEX, FUNCTION OF THE ESCRT-0 COMPLEX, SUBCELLULAR LOCATION.
[3]	"Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome." Bilodeau P.S., Winistorfer S.C., Kearney W.R., Robertson A.D., Piper R.C. J. Cell Biol. 163:237-243(2003) [PubMed: 14581452] [Abstract] Cited for: FUNCTION OF THE ESCRT-0 COMPLEX.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae." Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H. Traffic 5:194-210(2004) [PubMed: 15086794] [Abstract] Cited for: INTERACTION WITH RSP5; VPS23 AND VPS27, FUNCTION OF THE ESCRT-0 COMPLEX.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, MASS SPECTROMETRY.
[8]	"Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies." Ren J., Kee Y., Huibregtse J.M., Piper R.C. Mol. Biol. Cell 18:324-335(2007) [PubMed: 17079730] [Abstract] Cited for: FUNCTION, INTERACTION WITH RSP5 AND UBP7.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162 AND SER-365, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U10555 Genomic DNA. Translation: AAB68427.1.

PIR

S46798.

RefSeq

NP_011861.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PJW	X-ray	3.01	H	288-375	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1742N.

IntAct

P38753.

Genome annotation databases

Ensembl

YHL002W. Saccharomyces cerevisiae. [Contig view]

GeneID

856387.

GenomeReviews

Gene locus YHL002W in contig U00093_GR.

KEGG

sce:YHL002W.

NMPDR

fig|4932.3.peg.3005.

Organism-specific databases

CYGD

YHL002w.

SGD

S000000994. HSE1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P38753.

Gene expression databases

ArrayExpress

P38753.

GermOnline

YHL002W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR008942. ENTH_VHS.
IPR000108. Neu_cyt_fact_2.
IPR001452. SH3.
IPR013315. Spectrin_alpha.
IPR003903. UIM.
IPR002014. VHS.
[Graphical view]

Gene3D

G3DSA:1.25.40.90. ENTH_VHS. 1 hit.

Pfam

PF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]

PRINTS

PR00499. P67PHOX.
PR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.

ProDom

PD000066. SH3. 1 hit.
PD003686. VHS. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]

PROSITE

PS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P38753.

NextBio

981893.

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Entry information

Entry name

HSE1_YEAST

Accession

Primary (citable) accession number: P38753

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents