Reviewed, UniProtKB/Swiss-Prot P38759 (PEP11_YEAST)

Names and origin

Protein names

Recommended name:
    Vacuolar protein sorting-associated protein 29
Alternative name(s):
    Vesicle protein sorting 29
    Carboxypeptidase Y-deficient protein 11

Gene names

Name:	VPS29
Synonyms:	PEP11
Ordered Locus Names:	YHR012W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection.
Subunit structure	Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPS29, VPS26 and VPS35.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the VPS29 family.

Ontologies

Keywords
Biological process	Protein transport Transport
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Ref.3 Inferred from physical interaction. Source: SGD
Cellular component	endosome Ref.3 Inferred from physical interaction. Source: SGD retromer complex, inner shell Ref.3 Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VPS35	P34110	1	EBI-13092,EBI-20415

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Vacuolar protein sorting-associated protein 29		PRO_0000058305
Amino acid modifications
	Modified residue	207	1	Phosphothreonine

Sequences

	Sequence		Length	Mass (Da)
	P38759-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D190DBA2377E0653	FASTA	282	31,013
	10 20 30 40 50 60 MLLLALSDAH IPDRATDLPV KFKKLLSVPD KISQVALLGN STKSYDFLKF VNQISNNITI 70 80 90 100 110 120 VRGEFDNGHL PSTKKDKASD NSRPMEEIPM NSIIRQGALK IGCCSGYTVV PKNDPLSLLA 130 140 150 160 170 180 LARQLDVDIL LWGGTHNVEA YTLEGKFFVN PGSCTGAFNT DWPIVFDVED SDEAVTSEVD 190 200 210 220 230 240 KPTKENQSED DDAKGGSTGK EQPGSYTPKE GTAGERENEN ESNVKPENQF KEDEVDMSDS 250 260 270 280 DINGSNSPSF CLLDIQGNTC TLYIYLYVNG EVKVDKVVYE KE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the PEP11 locus in Saccharomyces cerevisiae." Friedrichsen D.M., Brands A., Hiller M.A., Bachhawat A., Jones E.W. Unpublished observations (JAN-1995) Cited for: IDENTIFICATION.
[3]	"A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast." Seaman M.N., McCaffery J.M., Emr S.D. J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract] Cited for: IDENTIFICATION IN THE RETROMER COMPLEX.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-207, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10400 Genomic DNA. Translation: AAB68947.1.
PIR	S46793.
RefSeq	NP_011876.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4205N.
IntAct	P38759.
Genome annotation databases
Ensembl	YHR012W. Saccharomyces cerevisiae. [Contig view]
GeneID	856403.
GenomeReviews	Gene locus YHR012W in contig U00093_GR.
KEGG	sce:YHR012W.
NMPDR	fig|4932.3.peg.3021.
Organism-specific databases
CYGD	YHR012w.
SGD	S000001054. VPS29.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38759.
Gene expression databases
GermOnline	YHR012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000979. UPF0025. [Graphical view]
TIGRFAMs	TIGR00040. yfcE. 1 hit.
ProtoNet	Search...
Other Resources
LinkHub	P38759.
NextBio	981940.

Entry information

Entry name

PEP11_YEAST

Accession

Primary (citable) accession number: P38759

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)