Reviewed, UniProtKB/Swiss-Prot P38911 (FKBP3_YEAST)

Names and origin

Protein names

Recommended name:
    FK506-binding nuclear protein
    EC=5.2.1.8
Alternative name(s):
    Peptidyl-prolyl cis-trans isomerase
      Short name=PPIase
    Proline rotamase
    Nucleolar proline isomerase
    FKBP-70

Gene names

Name:	FPR3
Synonyms:	NPI46
Ordered Locus Names:	YML074C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. FK506- and rapamycin-binding protein. Specifically binds nuclear localization sequences. May be involved in the assembly or folding of ribosomal proteins.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation	Inhibited by both FK506 and rapamycin.
Subcellular location	Nucleus › nucleolus.
Post-translational modification	Phosphorylated at tyrosine and dephosphorylated by the phosphotyrosine-specific phosphoprotein phosphatase PTP1.
Miscellaneous	Present with 9490 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the FKBP-type PPIase family. FKBP3/4 subfamily. Contains 1 PPIase FKBP-type domain.

Ontologies

Keywords
Cellular component	Nucleus
Molecular function	Isomerase Rotamase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	meiotic recombination checkpoint Inferred from direct assay. Source: SGD
Cellular component	nucleolus Ref.1 Ref.2 Inferred from direct assay. Source: SGD
Molecular function	peptidyl-prolyl cis-trans isomerase activity Ref.1 Ref.2 Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 411	411	FK506-binding nuclear protein
Regions
	Domain	324 – 411	88	PPIase FKBP-type
	Motif	256 – 271	16	Nuclear localization signal Potential
	Compositional bias	60 – 87	28	Asp/Glu-rich (highly acidic)
	Compositional bias	88 – 99	12	Lys-rich (highly basic)
	Compositional bias	101 – 119	19	Asp/Glu-rich (highly acidic)
	Compositional bias	173 – 248	76	Asp/Glu-rich (highly acidic)
	Compositional bias	250 – 298	49	Lys-rich (highly basic)
Amino acid modifications
	Modified residue	80	1	Phosphoserine
	Modified residue	81	1	Phosphoserine
	Modified residue	184	1	Phosphotyrosine; by CK2
	Modified residue	186	1	Phosphoserine; by CK2
Experimental info
	Sequence conflict	122	1	L → P in AAB04165. Ref.1
	Sequence conflict	240	1	E → EEE Ref.1
	Sequence conflict	335	1	L → F in AAB04165. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38911-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: A01D24DE0078FE11	FASTA	411	46,553
	10 20 30 40 50 60 MSDLLPLATY SLNVEPYTPV PAIDVTMPIT VRITMAALNP EAIDEENKPS TLRIIKRNPD 70 80 90 100 110 120 FEDDDFLGGD FDEDEIDEES SEEEEEEKTQ KKKKSKGKKA ESESEDDEED DDEDDEFQES 130 140 150 160 170 180 VLLTLSPEAQ YQQSLDLTIT PEEEVQFIVT GSYAISLSGN YVKHPFDTPM GVEGEDEDED 190 200 210 220 230 240 ADIYDSEDYD LTPDEDEIIG DDMDDLDDEE EEEVRIEEVQ EEDEEDNDGE EEQEEEEEEE 250 260 270 280 290 300 QKEEVKPEPK KSKKEKKRKH EEKEEEKKAK KVKKVEFKKD LEEGPTKPKS KKEQDKHKPK 310 320 330 340 350 360 SKVLEGGIVI EDRTIGDGPQ AKRGARVGMR YIGKLKNGKV FDKNTSGKPF AFKLGRGEVI 370 380 390 400 410 KGWDIGVAGM SVGGERRIII PAPYAYGKQA LPGIPANSEL TFDVKLVSMK N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel FK506- and rapamycin-binding protein (FPR3 gene product) in the yeast Saccharomyces cerevisiae is a proline rotamase localized to the nucleolus." Benton B.M., Zang J.-H., Thorner J. J. Cell Biol. 127:623-639(1994) [PubMed: 7525596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YNN 214.
[2]	"Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus." Shan X., Xue Z., Melese T. J. Cell Biol. 126:853-862(1994) [PubMed: 8051210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Purification of FKBP-70, a novel immunophilin from Saccharomyces cerevisiae, and cloning of its structural gene, FPR3." Manning-Krieg U.C., Henriquez R., Cammas F., Graff P., Gaveriaux S., Movva N.R. FEBS Lett. 352:98-103(1994) [PubMed: 7925954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Casein kinase II catalyzes tyrosine phosphorylation of the yeast nucleolar immunophilin Fpr3." Wilson L.K., Dhillon N., Thorner J., Martin G.S. J. Biol. Chem. 272:12961-12967(1997) [PubMed: 9148902] [Abstract] Cited for: PHOSPHORYLATION AT TYR-184 AND SER-186.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-81, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-81, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L34569 Genomic DNA. Translation: AAB04165.1. X79379 Genomic DNA. Translation: CAA55924.1. S73876 Genomic DNA. Translation: AAB31995.1. Z46373 Genomic DNA. Translation: CAA86504.1. AY693136 Genomic DNA. Translation: AAT93155.1.
PIR	S48647.
RefSeq	NP_013637.1.
3D structure databases
HSSP	HSSP built from PDB template 1KT1 based on UniProtKB Q9XSH5.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6578N.
IntAct	P38911.
Proteomic databases
PeptideAtlas	P38911.
Genome annotation databases
Ensembl	YML074C. Saccharomyces cerevisiae. [Contig view]
GeneID	854901.
GenomeReviews	Gene locus YML074C in contig Z71257_GR.
KEGG	sce:YML074C.
NMPDR	fig|4932.3.peg.4674.
Organism-specific databases
CYGD	YML074c.
SGD	S000004539. FPR3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38911.
Gene expression databases
ArrayExpress	P38911.
GermOnline	YML074C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001179. PPIase_FKBP. [Graphical view]
PANTHER	PTHR10516. PPIase_FKBP. 1 hit.
Pfam	PF00254. FKBP_C. 1 hit. [Graphical view]
PROSITE	PS50059. FKBP_PPIASE. 1 hit. [Graphical view]
ProDom	P38911. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P38911.
ProtoNet	Search...

Entry information

Entry name

FKBP3_YEAST

Accession

Primary (citable) accession number: P38911

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)