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Reviewed, UniProtKB/Swiss-Prot P38987 (TEM1_YEAST)

Last modified July 22, 2008. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protein TEM1
Gene names
Name: TEM1
Ordered Locus Names: YML064C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway.

Subunit structure

Interacts with CDC15 and AMN1. AMN1 and CDC15 compete for association with TEM1.

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Miro domain.

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Ontologies

Keywords

   Biological processCell cycle
Cell division
Mitosis
   LigandGTP-binding
Nucleotide-binding
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processregulation of exit from mitosis

Traceable author statement. Source: SGD

   Cellular componentspindle pole body

Traceable author statement. Source: SGD

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 245245Protein TEM1

Regions

Domain21 – 135115Miro
Nucleotide binding27 – 348GTP By similarity
Nucleotide binding75 – 795GTP By similarity
Nucleotide binding132 – 1354GTP By similarity

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Sequences

Sequence LengthMass (Da)Tools
P38987-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: F650C8946A03707C

FASTA24527,296
        10         20         30         40         50         60 
MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT QTLGVNFLKR 

        70         80         90        100        110        120 
KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF DLTRPETLSS IKEWYRQAYG 

       130        140        150        160        170        180 
LNDSAIPILV GTKYDLLIDL DPEYQEQISR TSMKYAQVMN APLIFCSTAK SINIQKIFKI 

       190        200        210        220        230        240 
ALAKIFNLTL TIPEINEIGD PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS 


PGVNT

« Hide

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References

« Hide 'large scale' references
[1]"The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase."
Shirayama M., Matsui Y., Toh-e A.
Mol. Cell. Biol. 14:7476-7482(1994) [PubMed: 7935462] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
Cell 112:697-709(2003) [PubMed: 12628189] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AMN1 AND CDC15.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

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Cross-references

Sequence databases

D38172 Genomic DNA. Translation: BAA07371.1.
Z38114 Genomic DNA. Translation: CAA86257.1.
AY557980 Genomic DNA. Translation: AAS56306.1.
PIRS48334.
RefSeqNP_013647.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1691N.
IntActP38987.

Proteomic databases

PeptideAtlasP38987.

Genome annotation databases

EnsemblYML064C. Saccharomyces cerevisiae. [Contig view]
GeneID854938.
GenomeReviewsGene locus YML064C in contig Z71257_GR.
KEGGsce:YML064C.
NMPDRfig|4932.3.peg.4684.

Organism-specific databases

CYGDYML064c.
SGDS000004529. TEM1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP38987.

Gene expression databases

ArrayExpressP38987.
GermOnlineYML064C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013684. Miro-like.
IPR001806. Ras_trnsfrmng.
IPR005225. Small_GTP_bd.
IPR017231. Small_GTPase_Tem1.
[Graphical view]
PfamPF08477. Miro. 1 hit.
[Graphical view]
PIRSFPIRSF037527. Small_GTPase_Tem1. 1 hit.
PRINTSPR00449. RASTRNSFRMNG.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
ProDomP38987.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

LinkHubP38987.
ProtoNetSearch...

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Entry information

Entry nameTEM1_YEAST
AccessionPrimary (citable) accession number: P38987
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents