Reviewed, UniProtKB/Swiss-Prot P39533 (ACON2_YEAST)

Names and origin

Protein names

Recommended name:
    Probable aconitate hydratase 2
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	ACO2
Ordered Locus Names:	YJL200C
ORF Names:	J0327

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	789 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Miscellaneous	Present with 4670 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro aconitate hydratase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 789	789	Probable aconitate hydratase 2		PRO_0000076649
Sites
	Metal binding	385	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	448	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	451	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39533-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: FABA4FE482D3F993	FASTA	789	86,583
	10 20 30 40 50 60 MLSSANRFYI KRHLATHANM FPSVSKNFQT KVPPYAKLLT NLDKIKQITN NAPLTLAEKI 70 80 90 100 110 120 LYSHLCDPEE SITSSDLSTI RGNKYLKLNP DRVAMQDASA QMALLQFMTT GLNQTSVPAS 130 140 150 160 170 180 IHCDHLIVGK DGETKDLPSS IATNQEVFDF LESCAKRYGI QFWGPGSGII HQIVLENFSA 190 200 210 220 230 240 PGLMMLGTDS HTPNAGGLGA IAIGVGGADA VDALTGTPWE LKAPKILGVK LTGKLNGWST 250 260 270 280 290 300 PKDVITKLAG LLTVRGGTGY IVEYFGEGVS TLSCTGMATI CNMGAEIGAT TSTFPYQEAH 310 320 330 340 350 360 KRYLQATNRA EVAEAADVAL NKFNFLRADK DAQYDKVIEI DLSAIEPHVN GPFTPDLSTP 370 380 390 400 410 420 ISQYAEKSLK ENWPQKVSAG LIGSCTNSSY QDMSRVVDLV KQASKAGLKP RIPFFVTPGS 430 440 450 460 470 480 EQIRATLERD GIIDIFQENG AKVLANACGP CIGQWNREDV SKTSKETNTI FTSFNRNFRA 490 500 510 520 530 540 RNDGNRNTMN FLTSPEIVTA MSYSGDAQFN PLTDSIKLPN GKDFKFQPPK GDELPKRGFE 550 560 570 580 590 600 HGRDKFYPEM DPKPDSNVEI KVDPNSDRLQ LLEPFKPWNG KELKTNVLLK VEGKCTTDHI 610 620 630 640 650 660 SAAGVWLKYK GHLENISYNT LIGAQNKETG EVNKAYDLDG TEYDIPGLMM KWKSDGRPWT 670 680 690 700 710 720 VIAEHNYGEG SAREHAALSP RFLGGEILLV KSFARIHETN LKKQGVLPLT FANESDYDKI 730 740 750 760 770 780 SSGDVLETLN LVDMIAKDGN NGGEIDVKIT KPNGESFTIK AKHTMSKDQI DFFKAGSAIN YIGNIRRNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77688 Genomic DNA. Translation: CAA54757.1. Z49475 Genomic DNA. Translation: CAA89495.1.
PIR	S46631.
RefSeq	NP_012335.1.
3D structure databases
HSSP	HSSP built from PDB template 7ACN based on UniProtKB P16276.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1376N.
IntAct	P39533.
Proteomic databases
PeptideAtlas	P39533.
Genome annotation databases
Ensembl	YJL200C. Saccharomyces cerevisiae. [Contig view]
GeneID	853230.
GenomeReviews	Gene locus YJL200C in contig Y13136_GR.
KEGG	sce:YJL200C.
NMPDR	fig|4932.3.peg.3298.
Organism-specific databases
CYGD	YJL200c.
SGD	S000003736. ACO2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P39533.
Gene expression databases
ArrayExpress	P39533.
GermOnline	YJL200C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR006248. Aconitase_mito-like. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF5. Aconitase_mito. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01340. aconitase_mito. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P39533.
NextBio	973443.

Entry information

Entry name

ACON2_YEAST

Accession

Primary (citable) accession number: P39533

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)