Reviewed, UniProtKB/Swiss-Prot P39986 (ATC6_YEAST)

Names and origin

Protein names

Recommended name:
    Probable cation-transporting ATPase 1
    EC=3.6.3.-

Gene names

Name:	SPF1
Ordered Locus Names:	YEL031W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + H(2)O = ADP + phosphate.
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1870 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type V subfamily.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cation transport Inferred from electronic annotation. Source: InterPro cellular calcium ion homeostasis Inferred from mutant phenotype. Source: SGD protein amino acid glycosylation Inferred from mutant phenotype. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1215	1215	Probable cation-transporting ATPase 1		PRO_0000046349
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 43	22	Potential
	Topological domain	44 – 49	6	Extracellular Potential
	Transmembrane	50 – 72	23	Potential
	Topological domain	73 – 191	119	Cytoplasmic Potential
	Transmembrane	192 – 214	23	Potential
	Topological domain	215 – 217	3	Extracellular Potential
	Transmembrane	218 – 236	19	Potential
	Topological domain	237 – 399	163	Cytoplasmic Potential
	Transmembrane	400 – 419	20	Potential
	Topological domain	420 – 432	13	Extracellular Potential
	Transmembrane	433 – 454	22	Potential
	Topological domain	455 – 993	539	Cytoplasmic Potential
	Transmembrane	994 – 1013	20	Potential
	Topological domain	1014 – 1020	7	Extracellular Potential
	Transmembrane	1021 – 1037	17	Potential
	Topological domain	1038 – 1055	18	Cytoplasmic Potential
	Transmembrane	1056 – 1079	24	Potential
	Topological domain	1080 – 1099	20	Extracellular Potential
	Transmembrane	1100 – 1122	23	Potential
	Topological domain	1123 – 1133	11	Cytoplasmic Potential
	Transmembrane	1134 – 1153	20	Potential
	Topological domain	1154 – 1170	17	Extracellular Potential
	Transmembrane	1171 – 1193	23	Potential
	Topological domain	1194 – 1215	22	Cytoplasmic Potential
Sites
	Active site	487	1	4-aspartylphosphate intermediate By similarity
	Metal binding	816	1	Magnesium By similarity
	Metal binding	820	1	Magnesium By similarity
Amino acid modifications
	Modified residue	324	1	Phosphoserine
	Modified residue	936	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P39986-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7A9960D34B91B5AE	FASTA	1,215	135,269
	10 20 30 40 50 60 MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY IKGPEWTFVY 70 80 90 100 110 120 LGTLVSLNIL VMLMPAWNVK IKAKFNYSTT KNVNEATHIL IYTTPNNGSD GIVEIQRVTE 130 140 150 160 170 180 AGSLQTFFQF QKKRFLWHEN EQVFSSPKFL VDESPKIGDF QKCKGHSGDL THLKRLYGEN 190 200 210 220 230 240 SFDIPIPTFM ELFKEHAVAP LFVFQVFCVA LWLLDEFWYY SLFNLFMIIS MEAAAVFQRL 250 260 270 280 290 300 TALKEFRTMG IKPYTINVFR NKKWVALQTN ELLPMDLVSI TRTAEESAIP CDLILLDGSA 310 320 330 340 350 360 IVNEAMLSGE STPLLKESIK LRPSEDNLQL DGVDKIAVLH GGTKALQVTP PEHKSDIPPP 370 380 390 400 410 420 PDGGALAIVT KTGFETSQGS LVRVMIYSAE RVSVDNKEAL MFILFLLIFA VIASWYVWVE 430 440 450 460 470 480 GTKMGRIQSK LILDCILIIT SVVPPELPME LTMAVNSSLA ALAKFYVYCT EPFRIPFAGR 490 500 510 520 530 540 IDVCCFDKTG TLTGEDLVFE GLAGISADSE NIRHLYSAAE APESTILVIG AAHALVKLED 550 560 570 580 590 600 GDIVGDPMEK ATLKAVGWAV ERKNSNYREG TGKLDIIRRF QFSSALKRSA SIASHNDALF 610 620 630 640 650 660 AAVKGAPETI RERLSDIPKN YDEIYKSFTR SGSRVLALAS KSLPKMSQSK IDDLNRDDVE 670 680 690 700 710 720 SELTFNGFLI FHCPLKDDAI ETIKMLNESS HRSIMITGDN PLTAVHVAKE VGIVFGETLI 730 740 750 760 770 780 LDRAGKSDDN QLLFRDVEET VSIPFDPSKD TFDHSKLFDR YDIAVTGYAL NALEGHSQLR 790 800 810 820 830 840 DLLRHTWVYA RVSPSQKEFL LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG 850 860 870 880 890 900 LKKLGEQRRL EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNPH YLKALESKGT 910 920 930 940 950 960 VITPEIRKAV EEANSKPVEV IKPNGLSEKK PADLASLLLN SAGDAQGDEA PALKLGDASC 970 980 990 1000 1010 1020 AAPFTSKLAN VSAVTNIIRQ GRCALVNTIQ MYKILALNCL ISAYSLSIIY MAGVKFGDGQ 1030 1040 1050 1060 1070 1080 ATVSGLLLSV CFLSISRGKP LEKLSKQRPQ SGIFNVYIMG SILSQFAVHI ATLVYITTEI 1090 1100 1110 1120 1130 1140 YKLEPREPQV DLEKEFAPSL LNTGIFIIQL VQQVSTFAVN YQGEPFRENI RSNKGMYYGL 1150 1160 1170 1180 1190 1200 LGVTGLALAS ATEFLPELNE AMKFVPMTDD FKIKLTLTLL LDFFGSWGVE HFFKFFFMDD 1210 KPSDISVQQV KIASK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT." Suzuki C., Shimma Y. Mol. Microbiol. 32:813-823(1999) [PubMed: 10361284] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-936, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-936, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18530 Genomic DNA. Translation: AAB64508.1.
PIR	S50428.
RefSeq	NP_010883.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6637N.
Proteomic databases
PeptideAtlas	P39986.
Genome annotation databases
Ensembl	YEL031W. Saccharomyces cerevisiae. [Contig view]
GeneID	856681.
GenomeReviews	Gene locus YEL031W in contig U00092_GR.
KEGG	sce:YEL031W.
NMPDR	fig|4932.3.peg.1936.
Organism-specific databases
CYGD	YEL031w.
SGD	S000000757. SPF1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P39986.
Gene expression databases
ArrayExpress	P39986.
GermOnline	YEL031W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001757. ATPase_P. IPR005834. Dehalogen-like_hydro. IPR008250. E1-E2_ATPase_reg. IPR006544. P-ATPase-V. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01494. ATPase_P-type. 3 hits. TIGR01657. P-ATPase-V. 1 hit.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P39986.
NextBio	982710.

Entry information

Entry name

ATC6_YEAST

Accession

Primary (citable) accession number: P39986

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)