Reviewed, UniProtKB/Swiss-Prot P40204 (RUXG_YEAST)

Names and origin

Protein names

Recommended name:
    Small nuclear ribonucleoprotein G
      Short name(s)=snRNP-G, Sm protein G, Sm-G, SmG

Gene names

Name:	SMX2
Synonyms:	SNP2
Ordered Locus Names:	YFL017W-A
ORF Names:	YFL017Bw

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	77 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site.
Subunit structure	Component of the Sm core complex, present in spliceosomal snRNP U1, U2, U4/U6 and U5. The core complex contains SMB1, SMD1, SMD2, SMD3, SME1, SMX3 and SMX2 (Sm proteins B, D1, D2, D3, E, F and G, respectively), and is probably a heptameric ring structure. SMX2 specifically interacts with SME1.
Subcellular location	Nucleus.
Miscellaneous	Present with 1400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the snRNP Sm proteins family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from physical interaction. Source: SGD
Cellular component	U4/U6 x U5 tri-snRNP complex Inferred from direct assay. Source: SGD snRNP U1 Ref.7 Inferred from direct assay. Source: SGD snRNP U5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 77	77	Small nuclear ribonucleoprotein G

Sequences

	Sequence		Length	Mass (Da)
	P40204-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 5C6208B8C5A9AB33	FASTA	77	8,482
	10 20 30 40 50 60 MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ 70 TVIRGNSIIS LEALDAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the LPD1 gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae: comparison between eukaryotic and prokaryotic sequences for related enzymes and identification of potential upstream control sites." Ross J., Reid G.A., Dawes I.W. J. Gen. Microbiol. 134:1131-1139(1988) [PubMed: 3058861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Kornfeld G.D. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Interactions within the yeast Sm core complex: from proteins to amino acids." Camasses A., Bragado-Nilsson E., Martin R., Seraphin B., Bordonne R. Mol. Cell. Biol. 18:1956-1966(1998) [PubMed: 9528767] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SME1.
[4]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Barrell B.G., Churcher C., Rajandream M.A. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry." Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M. Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997) [PubMed: 9012791] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[8]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed: 11804584] [Abstract] Cited for: CHARACTERIZATION OF THE SPLICEOSOME.
[9]	"Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin." Salgado-Garrido J., Bragado-Nilsson E., Kandels-Lewis S., Seraphin B. EMBO J. 18:3451-3462(1999) [PubMed: 10369684] [Abstract] Cited for: RNA-BINDING.
[10]	"Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain." Walke S., Bragado-Nilsson E., Seraphin B., Nagai K. J. Mol. Biol. 308:49-58(2001) [PubMed: 11302706] [Abstract] Cited for: SUBUNIT.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20880 Genomic DNA. No translation available. L31794 Genomic DNA. Translation: AAB63977.1. D50617 Genomic DNA. No translation available. Z46255 Genomic DNA. Translation: CAA86353.1. AY558463 Genomic DNA. Translation: AAS56789.1.
PIR	S48322.
RefSeq	NP_116636.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6683N.
IntAct	P40204.
Genome annotation databases
Ensembl	YFL017W-A. Saccharomyces cerevisiae. [Contig view]
GeneID	850528.
GenomeReviews	Gene locus YFL017W-A in contig D50617_GR.
KEGG	sce:YFL017W-A.
NMPDR	fig|4932.3.peg.2264.
Organism-specific databases
CYGD	YFL017w-a.
SGD	S000002965. SMX2.
Phylogenomic databases
HOGENOM	P40204.
Gene expression databases
ArrayExpress	P40204.
GermOnline	YFL017W-A. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006649. Euk_arc_LSM_core. IPR001163. LSM_snRNP_core. [Graphical view]
Pfam	PF01423. LSM. 1 hit. [Graphical view]
ProDom	PD020287. snRNP. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00651. Sm. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	P40204.
ProtoNet	Search...

Entry information

Entry name

RUXG_YEAST

Accession

Primary (citable) accession number: P40204

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	July 22, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)