Reviewed, UniProtKB/Swiss-Prot P40782 (CYPR1_CYNCA)

Names and origin

Protein names

Recommended name:
    Cyprosin
    EC=3.4.23.-

Gene names

Name:

CYPRO1

Organism

Cynara cardunculus (Cardoon)

Taxonomic identifier

4265 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Carduoideae › Cardueae › Cynara

Protein attributes

Sequence length	473 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Tissue specificity	Mostly present in the violet parts of styles and corollas of mature flowers.
Developmental stage	Expressed in early stages of floral development and switched off at maturation of the flower.
Sequence similarities	Belongs to the peptidase A1 family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Propeptide	‹1 – 33	›33	Activation peptide Potential
	Chain	34 – 473	440	Cyprosin
Regions
	Domain	281 – 384	104	Saposin B-type
Sites
	Active site	69	1	By similarity
	Active site	256	1	By similarity
Amino acid modifications
	Glycosylation	364	1	N-linked (GlcNAc...) Potential
	Glycosylation	411	1	N-linked (GlcNAc...) Potential
	Disulfide bond	82 ↔ 88		Potential
	Disulfide bond	247 ↔ 251		Potential
	Disulfide bond	392 ↔ 429		Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P40782-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 65F3232EBD06CB56	FASTA	473	51,564
	10 20 30 40 50 60 LKKRKVNILN HPGEHAGSND ANARRKYGVR GNFRDSDGEL IALKNYMDAQ YFGEIGIGTP 70 80 90 100 110 120 PQKFTVIFDT GSSNLWVPSS KCYFSVACLF HSKYRSTDST TYKKNGKSAA IQYGTGSISG 130 140 150 160 170 180 FFSQDSVKLG DLLVKEQDFI EATKEPGITF LAAKFDGILG LGFQEISVGD AVPVWYTMLN 190 200 210 220 230 240 QGLVQEPVFS FWLNRNADEQ EGGELVFGGV DPNHFKGEHT YVPVTQKGYW QFEMGDVLIG 250 260 270 280 290 300 DKTTGFCASG CAAIADSGTS LLAGTTTIVT QINQAIGAAG VMSQQCKSLV DQYGKSMIEM 310 320 330 340 350 360 LLSEEQPEKI CSQMKLCSFD GSHDTSMIIE SVVDKSKGKS SGLPMRCVPC ARWVVWMQNQ 370 380 390 400 410 420 IRQNETEENI INYVDKLCER LPSPMGESAV DCSSLSSMPN IAFTVGGKTF NLSPEQYVLK 430 440 450 460 470 VGEGATAQCI SGFTAMDVAP PHGPLWILGD VFMGQYHTVF DYGNLRVGFA EAA

References

[1]

"Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin." Cordeiro M.C., Xue Z.-T., Pietrzak M., Pais M.S., Brodelius P.E. Plant Mol. Biol. 24:733-741(1994) [PubMed: 8193298] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 178-186. Tissue: Flower bud.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69193 mRNA. Translation: CAA48939.1. Sequence problems.
PIR	S47096. T12049.
3D structure databases
HSSP	HSSP built from PDB template 1QDM based on UniProtKB P42210.
ModBase	Search...
Protein family/group databases
MEROPS	A01.020.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR007856. SapB_1. IPR008138. SapB_2. IPR008373. Saposin. IPR011001. Saposin_like. IPR008139. SaposinB. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits. G3DSA:1.10.225.10. Saposin_like. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. PF05184. SapB_1. 1 hit. PF03489. SapB_2. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN. PR01797. SAPOSIN.
ProDom	PD001732. SapB_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00741. SapB. 1 hit. [Graphical view]
PROSITE	PS00141. ASP_PROTEASE. 2 hits. PS50015. SAP_B. 2 hits. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CYPR1_CYNCA

Accession

Primary (citable) accession number: P40782

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)