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Reviewed, UniProtKB/Swiss-Prot P40965 (MSH4_YEAST)

Last modified July 22, 2008. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    MutS protein homolog 4
Gene names
Name: MSH4
Ordered Locus Names: YFL003C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length878 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in meiotic recombination. Facilitate crossovers between homologs during meiosis.

Subunit structure

Heterooligomer of MSH4 and MSH5.

Miscellaneous

Two distinct classes of crossovers have been demonstrated in budding yeast. Class I is MSH4/MSH5 dependent and exhibits crossover interference. Class II is MUS81/MMS4 dependent and exhibits no interference.

Sequence similarities

Belongs to the DNA mismatch repair mutS family.

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Ontologies

Keywords

   Biological processMeiosis
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processmeiotic recombination

Inferred from mutant phenotype. Source: SGD

   Cellular componentnuclear chromosome

Inferred from direct assay. Source: SGD

   Molecular functionDNA binding

Inferred from physical interaction. Source: SGD

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

CST9Q060321EBI-11371,EBI-30044
MSH5Q121752EBI-11371,EBI-11377

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 878878MutS protein homolog 4

Regions

Nucleotide binding634 – 6418ATP Potential

Experimental info

Sequence conflict811 – 8122MD → IH Ref.2 Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P40965-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 0FA1C1F29CC3D8CA

FASTA87899,221
        10         20         30         40         50         60 
MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL KKKTLQIAET 

        70         80         90        100        110        120 
TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP SIDCVLCCIY EVPRDISTRI 

       130        140        150        160        170        180 
GLCIINCNTG QMYLSDFMDS QIYIRVVHKL QIYQPTEILI PSSSLAPTVS KLATMIKFNV 

       190        200        210        220        230        240 
AETVKIEEGS RKCFNSQDGL AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI 

       250        260        270        280        290        300 
ISKSSRNLNA FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM 

       310        320        330        340        350        360 
GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD KLFSRLLCIN 

       370        380        390        400        410        420 
HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI SETKKIFNND AIMEIEKLIN 

       430        440        450        460        470        480 
SCINEDCVWA SSAIQLLNQR SYAVKSDSNG LLDVSRQIYK EVKEEFFREV EDLTAKNKIN 

       490        500        510        520        530        540 
LDHNYDSARG FYLRIKRQEF TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME 

       550        560        570        580        590        600 
EILLLSEETV DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI 

       610        620        630        640        650        660 
RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI CIMAQMGSGI 

       670        680        690        700        710        720 
PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL DDINTETLLI LDELGRGSSI 

       730        740        750        760        770        780 
ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ 

       790        800        810        820        830        840 
LTQKSVAIEN SGIRVVKKIF NPDIIAEAYN MDSLLKIAKA RTENEDSNGV VDQKTINQMK 

       850        860        870 
RIHNLVAILK ECAGNEKEPL TLGKLKEINS DFIENFEE

« Hide

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References

« Hide 'large scale' references
[1]"Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction."
Ross-Macdonald P., Roeder G.S.
Cell 79:1069-1080(1994) [PubMed: 8001134] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI."
Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T., Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T., Murakami Y.
Yeast 12:77-84(1996) [PubMed: 8789262] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]"Conserved properties between functionally distinct MutS homologs in yeast."
Pochart P., Woltering D., Hollingsworth N.M.
J. Biol. Chem. 272:30345-30349(1997) [PubMed: 9374523] [Abstract]
Cited for: INTERACTION WITH MSH5.

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Cross-references

Sequence databases

U13999 Genomic DNA. Translation: AAA65234.1.
D50617 Genomic DNA. Translation: BAA09235.1.
PIRA55201.

3D structure databases

HSSPHSSP built from PDB template 1EWR based on UniProtKB Q56215.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2426N.
IntActP40965.

Genome annotation databases

EnsemblYFL003C. Saccharomyces cerevisiae. [Contig view]
GenomeReviewsGene locus YFL003C in contig D50617_GR.
KEGGsce:YFL003C.

Organism-specific databases

CYGDYFL003c.
SGDS000001891. MSH4.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP40965.

Gene expression databases

ArrayExpressP40965.
GermOnlineYFL003C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007860. DNA_mismatch_repair_MutS_connt.
IPR007696. DNA_mismatch_repair_MutS_core.
[Graphical view]
PANTHERPTHR11361. MutS_C. 1 hit.
PfamPF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
ProDomPD001263. MutS_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
PROSITEPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
BLOCKSSearch...

Other Resources

LinkHubP40965.
ProtoNetSearch...

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Entry information

Entry nameMSH4_YEAST
AccessionPrimary (citable) accession number: P40965
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents