Reviewed, UniProtKB/Swiss-Prot P41185 (RPOC_BUCAP)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit beta'
      Short name(s)=RNAP subunit beta'
    EC=2.7.7.6
Alternative name(s):
    Transcriptase subunit beta'
    RNA polymerase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	BUsg_034

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	1413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1413	1413	DNA-directed RNA polymerase subunit beta'

Sequences

	Sequence		Length	Mass (Da)
	P41185-1 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 1D34A4DFD5971527	FASTA	1,413	157,681
	10 20 30 40 50 60 MKDLLKFLKA QTKNEDFDAI KISLASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR 70 80 90 100 110 120 IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVTQSKVRRE RMGHIELSSP TAHIWFLKSL 130 140 150 160 170 180 PSRIGLLLDM PLRDIERVLY FESYVVIETG MTNLEKRQIL TEEQYLDALE EFGDEFYATM 190 200 210 220 230 240 GAEAIQSLLK DINLVKECEN LRIELNETNS ETKRKKLTKR IKLLESFIQS NNKPEWMILT 250 260 270 280 290 300 VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLAAPDI IVRNEKRMLQ 310 320 330 340 350 360 EAIDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS GRSVITVGPY 370 380 390 400 410 420 LRLHQCGLPK KMALELFKPF IYGKLEVRGL ATTIKAAKKM VEREEAIVWD ILDEVIREHP 430 440 450 460 470 480 VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA 490 500 510 520 530 540 RALMMSTNNI LSPANGEPII VPSQDVVLGL YYMTREKING KGEGMILNGS NEAEKVYRLE 550 560 570 580 590 600 IAELHSLVKV RITEYKKNKD QSFIPKTKII NTTIGRAILW MIVPKGLPFS IVNQTLGKKD 610 620 630 640 650 660 ISKMLNTCYR ILGLKPTVAF ADQIMYTGFA YAARSGASVG IDDMVIPVKK LNIIHEAEIE 670 680 690 700 710 720 VAEIQEQFQS GLVTAGERYN KVIDIWAAAN ERVAKAMMEN LSTESVFNKK GEKQKQISFN 730 740 750 760 770 780 SIFMMADSGA RGSAAQIRQL AGMRGLMAKP DGSIIETPIT ANFREGLNVL QYFISTHGAR 790 800 810 820 830 840 KGLADTALKT ANSGYLTRRL VDVAQDLVVT QNDCGTHEGI LMTPLIEGGD VKEPLRERVL 850 860 870 880 890 900 GRVTAEKILI PNTENILIER NTLLNEQWCD LLEKNSIDNV KVRSVVNCET DFGVCAYCYG 910 920 930 940 950 960 RDLARGNLVN KGEAIGVIAA QSIGEPGTQL TMRTFHIGGA ASRAATESSI QIKNKGIINL 970 980 990 1000 1010 1020 NNAKSVTNSS GKIVITSRNV ELNIIDNFRR TKETYKVPYG AIMAKGHGEQ VNSGETVAKW 1030 1040 1050 1060 1070 1080 DPHTIPVITE VSGFVRFVDM IDGQSITRQA DELTGLSSIV VLDTAERMTI GKDLRPSLKI 1090 1100 1110 1120 1130 1140 VDRDGNDVLI SGTEMPAQYF LPGKAIVQLD DRVQISSGDT LARVPQESGG TKDITGGLPR 1150 1160 1170 1180 1190 1200 VADLFEARRP KELAILAEIS GIISFGKETK GKRRLIITPV DGSDAYEEMI PKWRQLNVFE 1210 1220 1230 1240 1250 1260 GERVERGDVI SDGPESPHDI LRLRGVQAVT KYIVNEVQEV YRLQGVKIND KHIEVIIRQM 1270 1280 1290 1300 1310 1320 LRKATVIKSG NSEFLDGEQV EFSRIKISNR ILNKQSKIPA TFSRDLLGIT KASLATESFI 1330 1340 1350 1360 1370 1380 SAASFQETTR VLTESAVAGK KDELRGLKEN VIVGRLIPAG TGYAYHKERL NRRHTVNTNQ 1390 1400 1410 IKPNNSSSQI SAEEASASLS ELLNSTLIQH DHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Sequence analysis of an aphid endosymbiont DNA fragment containing rpoB (beta-subunit of RNA polymerase) and portions of rplL and rpoC." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 25:283-290(1992) [PubMed: 1369199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-209.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67605.1. Z11913 Genomic DNA. Translation: CAA77971.1.
PIR	S32681.
RefSeq	NP_660394.1.
3D structure databases
HSSP	HSSP built from PDB template 1HQM based on UniProtKB Q9KWU6.
SMR	P41185. Positions 944-1128.
ModBase	Search...
Genome annotation databases
GeneID	1005850.
GenomeReviews	Gene locus BUsg_034 in contig AE013218_GR.
KEGG	bas:BUsg034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P41185.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG034-MON.
Family and domain databases
HAMAP	MF_01322. [Tree]
InterPro	IPR000722. RNA_pol_A. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. IPR012754. RpoC_beta_prime. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. rpoC_TIGR. 1 hit.
ProDom	P41185. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

RPOC_BUCAP

Accession

Primary (citable) accession number: P41185

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	August 30, 2002
Last modified:	July 22, 2008
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)