Reviewed, UniProtKB/Swiss-Prot P41500 (ACYP1_BOVIN)

Names and origin

Protein names

Recommended name:
    Acylphosphatase-1
    EC=3.6.1.7
Alternative name(s):
    Acylphosphate phosphohydrolase 1
    Acylphosphatase, organ-common type isozyme
    Acylphosphatase, erythrocyte/testis isozyme

Gene names

Name:	ACYP1
Synonyms:	ACYPE

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An acylphosphate + H(2)O = a carboxylate + phosphate.
Tissue specificity	Organ-common type isozyme is found in many different tissues.
Sequence similarities	Belongs to the acylphosphatase family. Contains 1 acylphosphatase-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative initiation
Molecular function	Hydrolase
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	acylphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Notes: It is uncertain whether isoform ACY1 is produced by alternative initiation or another biological event.

Isoform ACY2 (identifier: P41500-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform ACY1 (identifier: P41500-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Notes: Initiator methionine is removed. Contains an N-acetylalanine at position 2.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed; in isoform ACY2

Chain

2 – 101

100

Acylphosphatase-1

PRO_0000158534

Regions

Domain

11 – 101

91

Acylphosphatase-like

Sites

Active site

26

1

Potential

Active site

44

1

Potential

Amino acid modifications

Modified residue

2

1

N-acetylserine; in isoform ACY2

Natural variations

Alternative sequence

1 – 2

2

Missing in isoform ACY1.

VSP_026023

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

101

Helix Strand Turn

Details...

Beta strand

9 – 19

11

Beta strand

21 – 24

4

Helix

25 – 35

11

Beta strand

39 – 44

6

Beta strand

50 – 57

8

Helix

58 – 70

13

Beta strand

77 – 88

12

Beta strand

95 – 100

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform ACY2 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 92DC05E7BF0D86B7	FASTA	101	11,432
	10 20 30 40 50 60 MSMAEGDTLI SVDYEIFGKV QGVFFRKYTQ AEGKKLGLVG WVQNTDQGTV QGQLQGPASK 70 80 90 100 VRHMQEWLET KGSPKSHIDR ASFHNEKVIV KLDYTDFQIV K
	Isoform ACY1 [UniParc]. Checksum: 73DBBF3C27569C06		99	11,214
	10 20 30 40 50 60 MAEGDTLISV DYEIFGKVQG VFFRKYTQAE GKKLGLVGWV QNTDQGTVQG QLQGPASKVR 70 80 90 HMQEWLETKG SPKSHIDRAS FHNEKVIVKL DYTDFQIVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ACY2). Strain: Hereford. Tissue: Fetal pons.
[2]	"Purification, kinetic properties and primary structure of bovine erythrocyte acylphosphatase." Pazzagli L., Ikram U.K., Liguri G., Taddei N., Gentilini A., Cecchi C., Manao G., Cappugi G. Ital. J. Biochem. 42:233-245(1993) [PubMed: 8262761] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-101 (ISOFORM ACY2), ACETYLATION AT SER-2. Tissue: Erythrocyte.
[3]	"Bovine testis acylphosphatase: purification and amino acid sequence." Pazzagli L., Cappugi G., Camici G., Manao G., Ramponi G. J. Protein Chem. 12:593-601(1993) [PubMed: 8142002] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-101 (ISOFORM ACY2), PROTEIN SEQUENCE OF 4-101 (ISOFORM ACY1), ACETYLATION OF N-TERMINUS. Tissue: Testis.
[4]	"Crystal structure of common type acylphosphatase from bovine testis." Thunnissen M.M.G.M., Taddei N., Liguri G., Ramponi G., Nordlund P. Structure 5:69-79(1997) [PubMed: 9016712] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 4-101 (ISOFORM ACY1). Tissue: Testis.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BC114901 mRNA. Translation: AAI14902.1.

PIR

A61449.

RefSeq

NP_001039447.1.

UniGene

Bt.33942

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ACY	X-ray	1.80	A	4-101	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000013992. Bos taurus. [Contig view]

GeneID

507844.

KEGG

bta:507844.

Phylogenomic databases

HOVERGEN

P41500.

Family and domain databases

InterPro

IPR001792. Acylphosphatase.
[Graphical view]

PANTHER

PTHR10029. Acylphosphatase. 1 hit.

Pfam

PF00708. Acylphosphatase. 1 hit.
[Graphical view]

PRINTS

PR00112. ACYLPHPHTASE.

ProDom

PD001884. Acylphosphatase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00150. ACYLPHOSPHATASE_1. 1 hit.
PS00151. ACYLPHOSPHATASE_2. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P41500.

Entry information

Entry name

ACYP1_BOVIN

Accession

Primary (citable) accession number: P41500
Secondary accession number(s): Q1RMH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)